Plats1 thr 1079

Manufactured by Cell Signaling Technology

PLATS1 (Thr 1079) is a lab equipment product offered by Cell Signaling Technology. It is a phospho-specific antibody that detects endogenous levels of PLATS1 when phosphorylated at threonine 1079.

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Lab products found in correlation

P yap ser127, by Cell Signaling Technology (2 mentions) β actin ab8227, by Abcam (1 mentions) P lats1 ser909, by Cell Signaling Technology (1 mentions) Gemcitabine, by Eli Lilly (1 mentions) Anti α tubulin antibody, by Cell Signaling Technology (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions) Srebp1, by BD (1 mentions) Srebp2, by BD (1 mentions) Lats1, by Cell Signaling Technology (1 mentions) Sc 293183, by Santa Cruz Biotechnology (1 mentions) P taz ser89, by Cell Signaling Technology (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Gapdh, by Abcam (1 mentions)

4 protocols using plats1 thr 1079

Investigating AMPK and YAP Signaling

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Anti-AMPKα (no. 2532), pAMPKα (Thr 172) (no. 2535), YAP (no. 4912), pYAP (Ser 127) (no. 4911), LATS1 (no. 3477), LATS2 (no. 5888), pLATS1 (Ser 909) (no. 9157) and pLATS1 (Thr 1079) (no. 8654) were obtained from Cell Signaling. Anti-AMOTL1 (ab171976), HMGCR (ab174830) and β-actin (ab8227) were obtained from Abcam. Anti-pYAP(Ser61) and p-YAP(Ser94) were obtained from ABclonal (Wunhan, China). Nuciferine (≥98% purity) was purchased from Nature Standard Technical Service co., Ltd. (Shanghai, China). Nuciferine was dissolved in Dimethyl Sulfoxide (DMSO) then made into the storage solution with the concentration of 0.1 M. Working dilutions for nuciferine were prepared in culture medium, and DMSO was used as control. Gemcitabine was purchased from Eli Lilly (Indianapolis, IN, USA) and dissolved in sterile phosphate buffered solution (PBS) before use. MTT was obtained from Fluka Chemical Corp. (Ronkonkoma, NY, USA) and was dissolved in 0.01 M PBS.

Zhou L., Wang Q., Zhang H., Li Y., Xie S, & Xu M. (2019). YAP Inhibition by Nuciferine via AMPK-Mediated Downregulation of HMGCR Sensitizes Pancreatic Cancer Cells to Gemcitabine. Biomolecules, 9(10), 620.

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Western Blot Analysis of Apoptosis Regulators

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Western blot was performed according to a standard method, as described previously (38 (link)). Antibodies against BAX (#5023), BAD (#9239), BCL2L1 (#2764), BCL2 (#2872), p-MST1 (Thr183)/2(Thr180) (#3681), MST1 (#14946), p-LATS1(Thr1079) (#8654), LATS1 (#9153), p-YAP(Ser127) (#13008), YAP (#14074), NF2 (#6995), and RASSF1 (#86026) were purchased from Cell Signaling Technology, and TAZ from Abcam (ab224239), RASSF5 from Sigma (N5912). The membranes were stripped and reprobed with an anti–α-tubulin antibody (Cell Signaling Technology) as the loading control.

Liu X., Fu Q., Bian X., Fu Y., Xin J., Liang N., Li S., Zhao Y., Fang L., Li C., Zhang J., Dionigi G, & Sun H. (2021). Long Non-Coding RNA MAPK8IP1P2 Inhibits Lymphatic Metastasis of Thyroid Cancer by Activating Hippo Signaling via Sponging miR-146b-3p. Frontiers in Oncology, 10, 600927.

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Antibody-based Immunoblot Analysis

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Antibodies used for immunoblots were purchased from the indicated companies: p‐YAP (Ser127) (NO.13008), YAP (NO.14074), Lats1 (NO.3477), pLats1 (Ser909) (NO.9157), and pLats1 (Thr1079) (NO.8654) were from (Cell Signalling Technology). SREBP‐1 (Cat#557036), SREBP‐2 (Cat#557037) were from BD Biosciences.

Shu Z., Gao Y., Zhang G., Zhou Y., Cao J., Wan D., Zhu X, & Xiong W. (2019). A functional interaction between Hippo‐YAP signalling and SREBPs mediates hepatic steatosis in diabetic mice. Journal of Cellular and Molecular Medicine, 23(5), 3616-3628.

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Protein Extraction and Western Blot Analysis

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Protein extraction was carried out in ice-cold RIPA buffer with phosphatase and protease inhibitors. The protein concentrations were measured by the BCA protein assay kit (Beyotime, Haimen, China). PLA2G16 was detected using a polyclonal human PLA2G16 antibody (1 μg/mL, AF6190, R&D Systems, Inc.). Antibodies to TAZ (1:1000, sc-293183) and MST1 (1:1000, sc-515051) were purchased from Santa Cruz. LATS1 (1:100, #3477), p-LATS1 (Thr1079) (1:1000, #8654), p-TAZ (Ser89) (1:1000, #59971), and YAP (1:1000, #14074) were provided by Cell Signaling Technology. Antibodies to E-cadherin (1:500, cat. No. WL00941), N-cadherin (1:500; cat. No.WL01047), MMP-2(1:500; cat. No.WL1579), MMP-9 (1:500; cat. No.WL01580), Snail (1:500; cat. No.WL01863), Vimentin (1:500; cat. No.WL01960) and ZEB1 (1:500; cat. No.WL01657) were purchased from Wanleibio. Other antibodies were obtained from Abcam, including p-MST1 (T183) (1:2000, ab76323), p-YAP (S127) (1:10000, ab76252) and GAPDH (1:10000, ab181603).

Yang L., Zheng L., Xie X., Luo J., Yu J., Zhang L., Meng W., Zhou Y., Chen L., Ouyang D., Zhou H, & Tan Z. (2021). Targeting PLA2G16, a lipid metabolism gene, by Ginsenoside Compound K to suppress the malignant progression of colorectal cancer. Journal of Advanced Research, 36, 265-276.

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