Log In Sign up
The largest database of trusted experimental protocols

Alexa fluor 647 conjugated human fibrinogen

Manufactured by Thermo Fisher Scientific
Visit Supplier

Alexa Fluor 647–conjugated human fibrinogen is a fluorescence-labelled protein used for research purposes. It is produced by conjugating the Alexa Fluor 647 dye to human fibrinogen.

Automatically generated - may contain errors

Lab products found in correlation

Anti human cd61 antibody, by BD (3 mentions) Phe pro arg chloromethylketone ppack, by Prolytix (3 mentions) Corn trypsin inhibitor cti, by Prolytix (3 mentions) Sigmacote, by Merck Group (3 mentions) Alexa fluor 488 conjugated annexin 5, by Thermo Fisher Scientific (2 mentions) H gly pro arg pro oh gprp, by Merck Group (2 mentions) Collagen type 1, by Chrono-Log (2 mentions) Dade innovin prothrombin time reagent, by Siemens (2 mentions) Anti human cd61, by BD (2 mentions) Collagen type 1 chrono par aggregation reagent, by Chrono-Log (2 mentions) D dimer elisa kit, by Abcam (2 mentions) Sigmacote siliconizing reagent, by Merck Group (2 mentions) Sylgard 184 silicone elastomer kit, by Dow (2 mentions) Enzygnost f1 2 monoclonal kit, by Siemens (2 mentions) Dasatinib, by Selleck Chemicals (1 mentions) Gr144053, by Bio-Techne (1 mentions) Lipopolysaccharides (lps), by Novus Biologicals (1 mentions) Imiquimod, by Novus Biologicals (1 mentions) Dade innovin lipidated tissue factor tf, by Siemens (1 mentions) Malp 2, by Novus Biologicals (1 mentions)

5 protocols using alexa fluor 647 conjugated human fibrinogen

1

Platelet Activation Signaling Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents were obtained as follows: anti-human CD61 antibody (BD Biosciences, San Jose, CA. Cat#: 555754), Alexa Fluor 647–conjugated human fibrinogen (Life Technologies, Grand Island, NY. Cat#: F35200), Alexa Fluor 488 phospho-Src (Tyr418) polyclonal antibody (ThermoFisher Scientific, Waltham, MA. Cat#: 44–660A1), Dade Innovin prothrombin time (PT) reagent (Siemens, Malvern, PA. Cat#: B4212–40), collagen (type I; Chrono-Log, Havertown, PA. Cat#: 385), Sigmacote® (Millipore Sigma, Burlington, MA. Cat#: SL2–100ML), H-Gly-Pro-Arg-Pro-OH (GPRP; Millipore Sigma, Burlington, MA. Cat#: 03-34-0001), dasatinib (Selleckchem, Houston, TX. Cat#: S1021), GS-9973 (entospletinib) (Selleckchem, Houston, TX. Cat#: S7523), Alexa Fluor 488-conjugated annexin V (ThermoFisher Scientific, Waltham, MA. Cat#: A13201), GR144053 (Tocris Biosciences, Bristol, UK. Cat#: 1263), Phe-Pro-Arg-chloromethylketone (PPACK, Haematologic Technologies, Essex Junction, VT. Cat#: FPRCK-01) and corn trypsin inhibitor (CTI, Haematologic Technologies, Essex Junction, VT. Cat#: CTI-01).
Zhang Y, & Diamond S.L. (2020). Src family kinases inhibition by dasatinib blocks initial and subsequent platelet deposition on collagen under flow, but lacks efficacy with thrombin generation.. Thrombosis research, 192, 141-151.
+ Open protocol
+ Expand
2

Platelet Activation and Thrombosis Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents were obtained as follows: anti-human CD61 antibody (BD Biosciences, San Jose, CA. Cat#: 555754), Alexa Fluor 647–conjugated human fibrinogen (Life Technologies, Grand Island, NY. Cat#: F35200), Alexa Fluor 488-conjugated annexin V (ThermoFisher Scientific, Waltham, MA. Cat#: A13201), collagen (type I; Chrono-Log, Havertown, PA. Cat#: 385), Dade Innovin lipidated tissue factor (TF, Siemens, Malvern, PA, USA), Sigmacote® (Millipore Sigma, Burlington, MA. Cat#: SL2-100ML), Phe-Pro-Arg-chloromethylketone (PPACK, Haematologic Technologies, Essex Junction, VT. Cat#: FPRCK-01), corn trypsin inhibitor (CTI, Haematologic Technologies, Essex Junction, VT, USA), Pam3CKS4 (NOVUS Biologicals, CO. Cat#: NBP2-25297), MALP-2 (NOVUS Biologicals, CO. Cat#: NBP2-26219), Polyinosinic-polycytidylic acid HMW (NOVUS Biologicals, CO. Cat#: NBP2-25288), LPS (NOVUS Biologicals, CO. Cat#: NBP2-25295), Imiquimod (NOVUS Biologicals, CO. Cat#: NBP2-26228), CpG oligodeoxynucleotides (NOVUS Biologicals, CO. Cat#: NBP2-26232), Vesatolimod (MedChemExpress, NJ. Cat#: HY-15601), GSK2245035 (MedChemExpress, NJ. Cat#: HY-118250), Bay 11-7082 (Millipore Sigma, MO. Cat#: B5556), and IKK inhibitor VII (Millipore Sigma, MO. Cat#: 401486).
Liu Y, & Diamond S.L. (2023). Activation of Most Toll-Like Receptors in Whole Human Blood Attenuates Platelet Deposition on Collagen under Flow. Journal of Immunology Research, 2023, 1884439.
+ Open protocol
+ Expand
3

Evaluating Fibrin Formation and Platelet Function

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following reagents were obtained: Sigmacote (Millipore Sigma, Cat# SL2-100ML), collagen (type I; Chrono-Log, Cat# 385), Dade Innovin prothrombin time reagent (Siemens, Cat# B4212-40), corn trypsin inhibitor (CTI; Haematologic Technologies, Cat# CTI-01), fluorescently labeled fragment derived from α2-AP (Ac-GNQEQVSPLTLLKW-C[IAF], α2-APF, CPC Scientific), antihuman CD61 antibody (BD Biosciences, Cat# 555754), Alexa Fluor 647 Conjugated Human Fibrinogen (Life Technologies, Cat# F35200), H-Gly-Pro-Arg-Pro-OH (GPRP; Millipore Sigma, Cat# 03-34-0001), Phe-Pro-Arg-chloromethylketone (PPACK; Haematologic Technologies, Cat# FPRCK-01), 1,3,4,5-tetramethyl-2-([2-oxopropyl]thio) imidazolium chloride (T101; Zedria GmbH), Recombinant Human Tissue Plasminogen Activator Protein (tPA; Abcam, Cat# ab92637), and rhosin (Rho inhibitor; Millipore Sigma, CAS 1173671-63-0).
Liu Y., Crossen J., Stalker T.J, & Diamond S.L. (2023). Fluorescent peptide for detecting factor XIIIa activity and fibrin in whole blood clots forming under flow. Research and Practice in Thrombosis and Haemostasis, 8(1), 102291.
+ Open protocol
+ Expand
4

Platelet Aggregation and Coagulation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following reagents were obtained: anti-human CD61 (BD Biosciences, San Jose, CA), Alexa Fluor647-conjugated human fibrinogen (Life Technologies, Grand Island, NY), collagen type I Chrono-Par aggregation reagent (Chrono-log, Havertown, PA), CTI (corn trypsin inhibitor; Hematologic Technologies, Essex Junction, VT), Phe-Pro-Arg-chloromethylketone (PPACK; Hematologic Technologies, Essex Junction, VT), Dade Innovin prothrombin time reagent (Siemens, Malvern, PA), Enzygnost F1+2 monoclonal kit (Siemens Healthcare Diagnostic, Tarrytown, NY), D-dimer ELISA Kit (Abcam, Cambridge, MA), human FPA (fibrinopeptide-A), ELISA Kit (MyBioSource, San Diego, CA), EDTA (Sigma, St. Louis, MO), H-Gly-Pro-Arg-Pro-OH (EMD Chemicals, San Diego, CA), fluorescein Phe-Pro-Arg-chloromethylketone (fluorescein-PPACK; Hematologic Technologies, Essex Junction, VT), Sigmacote siliconizing reagent (Sigma, St. Louis, MO), and Sylgard 184 Silicone Elastomer kit (Dow Corning, Auburn, MI). O1A6 FXI antibody was a gift from Dr Andras Gruber (Department of Biomedical Engineering, Oregon Health and Science University). See the online-only Data Supplement for catalog numbers.
Zhu S., Chen J, & Diamond S.L. (2018). Establishing the Transient Mass Balance of Thrombosis: From Tissue Factor to Thrombin to Fibrin Under Venous Flow. Arteriosclerosis, Thrombosis, and Vascular Biology, 38(7), 1528-1536.
+ Open protocol
+ Expand
5

Platelet and Coagulation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following reagents were obtained: Anti-human CD61 (BD Biosciences, San Jose, CA), Alexa Fluor®647 conjugated human fibrinogen (Life Technologies, Grand Island, NY), Collagen Type I Chrono-Par™ aggregation reagent (Chrono-log, Havertown, PA), corn trypsin inhibitor (CTI, Haematologic Technologies, Essex Junction, VT), Phe-Pro-Arg-chloromethylketone (PPACK; Hematologic Technologies, Essex Junction, VT), Dade® Innovin® PT reagent (Siemens, Malvern, PA), Enzygnost® F1+2 monoclonal kit (Siemens Healthcare Diagnostic, Tarrytown, NY), D-dimer ELISA kit (Abcam, Cambridge, MA), Human Fibrinopeptide A (FPA) ELISA Kit (MyBioSource, San Diego, CA), ethylenediaminetetraacetic acid (EDTA, Sigma, St. Louis, MO), H-Gly-Pro-Arg-Pro-OH (GPRP, EMD Chemicals, San Diego, CA), Fluorescein Phe-Pro-Arg-chloromethylketone (fluorescein-PPACK; Hematologic Technologies, Essex Junction, VT), Sigmacote® siliconizing reagent (Sigma, St. Louis, MO), and Sylgard® 184 Silicone Elastomer kit (Dow Corning, Auburn, MI). O1A6 FXI antibody was a gift from Dr. Andras Gruber (Department of Biomedical Engineering, Oregon Health and Science University). See Supplement for catalog numbers.
Zhu S., Chen J, & Diamond S.L. (2018). Establishing the Transient Mass Balance of Thrombosis: From Tissue Factor to Thrombin to Fibrin Under Venous Flow. Arteriosclerosis, Thrombosis, and Vascular Biology, 38(7), 1528-1536.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!