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2 protocols using recombinant mouse csf1 416 ml

1

Bleomycin-Induced Lung Fibrosis Model

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Endotoxin-free RPMI 1640 (15–040) and DPBS (21–031) were purchased from Corning Cellgro. Endotoxin-free (certified less than 0.06 EU/ml) fetal bovine serum (FBS, S11150) was obtained from Atlanta Biologicals. Recombinant mouse CSF1 (416-ML) was purchased from R&D Systems. Anti-mouse CD45 antibody (clone 30-F11, 103112), Alexa488 anti-mouse F4/80 (clone BM8, 123120), PerCP/Cy5.5 anti-mouse CXCR4 (clone L276F12, 146510), and APC anti-mouse CD115 (CSF-1R/M-CSF-R) (clone AFS98, 135510) were purchased from Biolegend. Anti-rabbit αSMA (ab5694) and anti-mouse Collagen I (clone COL-1, ab6308) were obtained from Abcam. LC3B (D11) XP Rabbit mAb (3868) and Alexa Fluor® 488 conjugate secondary antibody was purchased from Cell Signaling Technology. FITC anti-mouse α-SMA antibody (clone 1A4, F3777) was purchased from Sigma-Aldrich. Cytofix/Cytoperm solution (554714) was purchased from BD Biosciences. Primers and SYBR Green Master Mix (4385610) for quantitative real-time polymerase chain reaction (qRT-PCR) were purchased from Thermofisher Scientific unless indicated otherwise. Bleomycin was obtained from Sicor Pharmaceuticals (Irvine, CA).
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2

Bleomycin-Induced Lung Fibrosis Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
Endotoxin-free RPMI 1640 (15–040) and DPBS (21–031) were purchased from Corning Cellgro. Endotoxin-free (certified less than 0.06 EU/ml) fetal bovine serum (FBS, S11150) was obtained from Atlanta Biologicals. Recombinant mouse CSF1 (416-ML) was purchased from R&D Systems. Anti-mouse CD45 antibody (clone 30-F11, 103112), Alexa488 anti-mouse F4/80 (clone BM8, 123120), PerCP/Cy5.5 anti-mouse CXCR4 (clone L276F12, 146510), and APC anti-mouse CD115 (CSF-1R/M-CSF-R) (clone AFS98, 135510) were purchased from Biolegend. Anti-rabbit αSMA (ab5694) and anti-mouse Collagen I (clone COL-1, ab6308) were obtained from Abcam. LC3B (D11) XP Rabbit mAb (3868) and Alexa Fluor® 488 conjugate secondary antibody was purchased from Cell Signaling Technology. FITC anti-mouse α-SMA antibody (clone 1A4, F3777) was purchased from Sigma-Aldrich. Cytofix/Cytoperm solution (554714) was purchased from BD Biosciences. Primers and SYBR Green Master Mix (4385610) for quantitative real-time polymerase chain reaction (qRT-PCR) were purchased from Thermofisher Scientific unless indicated otherwise. Bleomycin was obtained from Sicor Pharmaceuticals (Irvine, CA).
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