The colorimetric identification of cholesterol and subsequent analysis of dairy products was performed using a single beam Shimadzu UVmini-1240 UV Visible Spectrophotometer (Shimadzu Corporation, Kyoto, Japan), fitted with 10 × 10 mm cuvette holder, and scans within a wavelength range of 190–1100 nm produced from a Deuterium (D2) lamp and a Tungsten Halogen (WI) lamp. Data acquisition and results analysis were facilitated with Shimadzu UV Data Manager Software installed on a windows computer system. Analytical balance (Kern, Germany/WD140050809), melting point apparatus (Stuart Apparatus SMP10, UK), and a refrigerator (Whirlpool, Model WRT348FMEZ, USA) were among other equipment employed for the study.
Uvmini 1240 uv visible spectrophotometer
Manufactured by Shimadzu
Sourced in Japan
The UVmini-1240 is a UV-Visible Spectrophotometer manufactured by Shimadzu. It is a compact and easy-to-use instrument designed for basic absorption measurements. The UVmini-1240 covers a wavelength range of 190 to 1100 nm and can perform various spectroscopic analyses.
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4 protocols using uvmini 1240 uv visible spectrophotometer
1
Colorimetric Cholesterol Identification in Dairy
2
Nanofiber Drug Encapsulation Efficiency
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Nanofibers with specific weights were dissolved in proper solvent systems (distilled water for PVA, acetic acid (1% v/v) for CS, and DCM/DMF/ACT (4:5:1 v/v) for PCL) and then were examined for ultraviolet (UV) absorbance at a maximum wavelength of 365 nm using a UV mini-1240 UVvisible spectrophotometer (Shimadzu, Japan). The EE% was calculated by Eq. 4, where W M stands for the weight of the measured drug in nanofibers, and W U stands for the weight of the drug used in the preparation of nanofibers. The test was repeated for 3 samples and a mean value was reported.
3
Test Kit Preparation and Fermentation Mixture Analysis
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The test kit preparation follows the general instructions from R-Biopharm and Roche Diagnostics available on the website of R-Biopharm (https://food.r-biopharm.com).
The preliminary preparation of the fermentation mixture follows the procedure described above (NMR sample preparation). Sample solutions with potentially higher concentrations have been diluted with deionized water. UV spectra have been recorded at 340 nm with a Shimadzu UV Visible Spectrophotometer UVmini-1240.
The preliminary preparation of the fermentation mixture follows the procedure described above (NMR sample preparation). Sample solutions with potentially higher concentrations have been diluted with deionized water. UV spectra have been recorded at 340 nm with a Shimadzu UV Visible Spectrophotometer UVmini-1240.
4
Comprehensive Material Characterization Protocols
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Color reader (Minolta CR-10, Minolta, Japan), analytical balances (Mettler Toledo Inc., USA), Shaker water-bath (Memmert WNB14, Germany), Centrifuge (Thermo Scientific SL40R, Thermo Fisher Scientific, Inc.), viscometer (NDJ-1 rotational viscometer, China), Shimadzu UV-Visible Spectrophotometer UVmini-1240 (Shimadzu cooperation, Kyoto, Japan), Oven (Memmert, Germany), Vortex (IKA Vortex 3, ProfilLab24 GmbH, Berlin, Germany), Electrical Stove (Maspion, Indonesia), Shaker (SI-1500 Orbital-Genie Orbital Shaker, Marshall Scientific LLC)., Centrifugal mill (Workshop local made, CM 1 model, Indonesia), Disk mill (FFC 45 model, China), Micro-mill assisted cyclone separator (Workshop local made, MM 1 model, Indonesia).
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