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4 nitrophenyl sulfate

Manufactured by Merck Group
Sourced in United States

4-nitrophenyl sulfate is a laboratory chemical compound used as a substrate in various biochemical and enzymatic assays. It is a colorless, crystalline solid that is soluble in water and organic solvents. The compound is commonly used to detect and quantify the activity of specific enzymes, such as sulfatases, that can cleave the sulfate group from the 4-nitrophenyl moiety, releasing a colored 4-nitrophenol product that can be measured spectrophotometrically.

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2 protocols using 4 nitrophenyl sulfate

1

Analytical Techniques for Bacterial Metabolites

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Gastrodin (>98%) was purchased from Tokyo Chemical Industry. 4-HBA (≥98%), erythromycin, oxytetracycline hydrochloride, cefadroxil, 4-nitrophenyl β-d-glucuronide, 4-nitrophenyl sulfate, and 4-nitrophenyl β-d-glucopyranoside were purchased from Sigma-Aldrich. The anaerobe gas-generating system was purchased from Becton, Dickinson and Company. HPLC-grade methanol was purchased from J. T. Bakers (Central Valley, PA, USA). All other chemicals used were of reagent grades and were used as received.
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2

Quantitative Analysis of Antidiabetic Compounds

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Voglibose (>98.0%) was purchased from Tokyo Chemical Industries (Tokyo, Japan). Erythromycin, oxytetracycline hydrochloride, cefadroxil, 4-nitrophenyl β-D-glucopyranoside, 4-nitrophenyl β-D-glucuronide, 4-nitrophenyl sulfate, soluble starch, ammonium acetate, streptozotocin, and telmisartan, the internal standard (IS), were purchased from Sigma-Aldrich (St. Louis, MO, USA). High-performance liquid chromatography (HPLC)-grade acetonitrile was purchased from J. T. Bakers (Central Valley, PA, USA). An anaerobe gas generating system was purchased from Becton, Dickinson and Company (Sparks, MD, USA). A blood glucose monitoring system was purchased from Bayer Contour TS (Mishawaka, IN, USA). Potassium phosphate monobasic and potassium phosphate dibasic were purchased from Duksan (Seoul, Korea). All other chemicals were of analytical grades and used as received.
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