Tcs sp5 2

The morphologies of the nano-/micro-fibrous materials were characterized by SEM (HITACHI SU800), laser scanning confocal microscope (Leica TCS SP5 II) and fluorescent microscope (Nikon Eclipse Ni-U). The crystallinity of the PVA sample was characterized by wide angle X-ray diffraction (WAXD, D/Max 2550 VB PC, Rigaku). UV absorption measurements were performed on a Shimadzu UV 3600. PL spectra were collected on a PTI QM/TM. The quantum yields were measured on a HAMAMASTU fluorescence spectrometer. For demonstrating the fluorescence sensitivity of the AIE/polymer fibers, the samples were placed in a self-made moisture chamber and incubated for 1 h before characterization. The moisture (RH) was adjusted by saturated salt solutions. The RH from 11% to 95% was adjusted by saturated salt solutions of LiCl, MgCl₂, NaBr, NaCl and KNO₃, respectively. Fluorescent and optical images were collected on a Cannon digital camera. A MATLAB program was used to convert the optical images from RGB color space into CIE x and y chromaticity values [50 (link)].

To perform immunostaining, mice were sacrificed and transcardially perfused with ice cold 4% paraformaldehyde (Electron Microscopy Sciences, USA), followed by incubation in 30% sucrose (Sigma-Aldrich, USA). After the brain was incubated in the sucrose solution, 60-μm-thick slices were cut with a cryostat. The slices were imaged using a confocal laser scanning microscope (Leica TCS SP5II, Germany or Nikon A1R, Japan) to determine the GCaMP expression patterns in the cerebellum. The slices were counterstained with DAPI (Thermo Fisher Scientific, USA).