HPaSteCs were obtained from ScienCell Research Laboratories (Carlsbad, CA, USA). The pancreatic cancer cell lines, PANC-1, Capan-2, and AsPC-1, were acquired from the Korean Cell Line Bank (KCLB, Seoul, Korea). HPaSteC and PANC-1 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Welgene, Daegu, Korea) supplemented with 10% fetal bovine serum (FBS; Welgene) and 1% antibiotic-antimycotic solution (Welgene). Capan-2 and AsPC-1 cells were maintained in RPMI-1640 medium (Welgene) supplemented with 10% FBS (Welgene) and 1% antibiotic-antimycotic solution (Welgene). The human colorectal adenocarcinoma cell lines, HT-29 and SW620, were obtained from the KCLB. Both HT-29 and SW620 cells were cultured in RPMI 1640 medium (Welgene) supplemented with 10% FBS (Welgene) and 1% antibiotic-antimycotic solution (Welgene).
Hpastec
Manufactured by ScienCell
Sourced in United States
The HPaSteC is a specialized laboratory equipment designed for the culture and expansion of human primary astrocytes. It provides a controlled environment for the cultivation and maintenance of these cells, which are essential for various research and clinical applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Welgene (1 mentions)
Aspc 1, by Welgene (1 mentions)
Sw620, by Welgene (1 mentions)
Panc 1, by Welgene (1 mentions)
Capan 2, by Korean Cell Line Bank (1 mentions)
Aspc 1, by Korean Cell Line Bank (1 mentions)
Panc 1, by Korean Cell Line Bank (1 mentions)
Antibiotic antimycotic solution, by Welgene (1 mentions)
Rpmi 1640 medium, by Welgene (1 mentions)
Dulbecco s modified eagle s medium dmem, by Welgene (1 mentions)
Fetal bovine serum (fbs), by ScienCell (1 mentions)
Bxpc 3 cells, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Horse serum, by American Type Culture Collection (1 mentions)
Stellate cell growth supplement, by ScienCell (1 mentions)
Penicillin streptomycin, by ScienCell (1 mentions)
Stellate cell medium, by ScienCell (1 mentions)
Bxpc 3, by National Collection of Authenticated Cell Cultures (1 mentions)
Mia paca 2, by American Type Culture Collection (1 mentions)
5 protocols using hpastec
1
Cell Culture Protocols for Pancreatic and Colorectal Cancer
2
Isolation and Culture of Pancreatic Cell Lines
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HPaSteCs were purchased from ScienCell Research Laboratories (San Diego, CA, USA) and cultured in stellate cell medium (ScienCell Research Laboratories) supplemented with 2% fetal bovine serum (ScienCell Research Laboratories), 1% stellate cell growth supplement (ScienCell Research Laboratories), and 1% penicillin/streptomycin (ScienCell Research Laboratories) in a humidified atmosphere at 37 °C with 5% CO2.
MIA PaCa-2 and PANC-1 cells were acquired from American Tissue Cell Culture (ATCC; Manassas, VA, USA), and OCUP-A2 is a PC cell line established by our group from a patient with malignant pancreatic neoplasm and liver metastasis [78 (link)]. These cell lines were grown in Dulbecco’s Modified Eagle’s Medium (Gibco, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (Gibco) and 2.5% horse serum (ATCC; for MIA PaCa-2 cells only). BxPC-3 cells were acquired from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan) and cultured in RPMI 1640 medium with 10% heat-inactivated FBS (Gibco) in a humidified atmosphere at 37 °C with 5% CO2. Details of cell-based assays are provided in theSupplemental Materials .
MIA PaCa-2 and PANC-1 cells were acquired from American Tissue Cell Culture (ATCC; Manassas, VA, USA), and OCUP-A2 is a PC cell line established by our group from a patient with malignant pancreatic neoplasm and liver metastasis [78 (link)]. These cell lines were grown in Dulbecco’s Modified Eagle’s Medium (Gibco, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (Gibco) and 2.5% horse serum (ATCC; for MIA PaCa-2 cells only). BxPC-3 cells were acquired from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan) and cultured in RPMI 1640 medium with 10% heat-inactivated FBS (Gibco) in a humidified atmosphere at 37 °C with 5% CO2. Details of cell-based assays are provided in the
3
Culturing and Xenograft of Pancreatic Cells
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The human pancreatic stellate cell line HPaSteC was obtained from ScienCell Research Laboratories and cultured in DMEM, while the human pancreatic cancer cell line BxPC-3 was provided by the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) in RPMI 1640 medium. All culture medium contained 10% FBS and 1% penicillin–streptomycin. Both cell lines were cultured at 37 °C in a 95% humidified atmosphere containing 5% CO2.
Male BALB/c nude mice (20 ± 2 g), supplied by Shanghai Sippr-BK Laboratory Animal Co., Ltd. (Shanghai, China), were acclimatized at 25 °C and 55% humidity under natural light/dark conditions. All animal experiments were carried out under the guidelines approved by the Institutional Animal Care and Use Committee of Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Male BALB/c nude mice (20 ± 2 g), supplied by Shanghai Sippr-BK Laboratory Animal Co., Ltd. (Shanghai, China), were acclimatized at 25 °C and 55% humidity under natural light/dark conditions. All animal experiments were carried out under the guidelines approved by the Institutional Animal Care and Use Committee of Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
4
Isolation and Cultivation of Primary Human Pancreatic Stellate Cells
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Primary human pancreatic stellate cells (hPSCs) were obtained from tumor tissue sampled from surgical resection specimens with PDAC. Human pancreatic stellate cells were isolated and cultured by the outgrowth method as previously described [23 (link),24 (link)]. Cultures were established and propagated from three different patients (designated hPSC-1, hPSC-2, and hPSC-3). The purity of the hPSCs was assessed by morphology and demonstration of α-SMA and vimentin expression. All experiments were performed using cell cultures between passage 3 and 8. The PDAC cell lines BxPC-3 and MIA PaCa-2 were purchased from ATCC (Manassas, VA, USA). The hPSC and PDAC cell lines were cultured and maintained in DMEM supplemented with 10% FBS, 1% Pen-Strep, and 1% amphotericin B. HPaSteC (Human Pancreatic Stellate Cells, fibroblastic cells isolated from pancreas of a 22-week old, fetal, non-diseased, male donor) were purchased from ScienCell Research Laboratories (San Diego, CA, USA), cultured, and maintained according to the supplier’s protocol.
Immortalized PSC cell lines from human RLT-PSC (referred to as i-hPSC) [18 (link)] and from mice (referred to as i-mPSC C2 and i-mPSC C3) [20 (link)] were kindly provided by Prof. J-M. Löhr, Karolinska Institute, Sweden. The immortalized cells were cultured and maintained in DMEM/F-12 supplemented with 10% FBS, 1% Pen-Strep and 1% amphotericin B.
Immortalized PSC cell lines from human RLT-PSC (referred to as i-hPSC) [18 (link)] and from mice (referred to as i-mPSC C2 and i-mPSC C3) [20 (link)] were kindly provided by Prof. J-M. Löhr, Karolinska Institute, Sweden. The immortalized cells were cultured and maintained in DMEM/F-12 supplemented with 10% FBS, 1% Pen-Strep and 1% amphotericin B.
5
ATRA-Induced Activation of Pancreatic Stellate Cells
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Primary, culture-activated human pancreatic stellate cells (passage 5-8 PSCs, HPaSteC, #3830, ScienCell, CA, USA) were cultured for at least two media changes and until cells reached a confluency between 65% and 75%. Cells were incubated with Dulbecco's Modified Eagle's Medium (DMEM-F12HAM) (Sigma-Aldrich, UK), with 10% Foetal Bovine Serum Heat Inactivated (FBS) (Gibco, UK), 1% penicillin/streptomycin (Sigma-Aldrich, UK), and 1% fungizone Anphotericin B (Gibco, UK). For the all-trans retinoic acid (ATRA) (#R2625, Sigma-Aldrich, UK) treatment condition, cells were exposed to ATRA dissolved in ethanol at a concentration of 1 μM for 10 days with medium changed every 24 h and treatment performed in subdued light. A control PSC group for ATRA treatment was established by adding 1 μl/ml of ethanol to the control media during the 10 days.
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