The human astrocytes were purchased from Thermo Fisher Scientific (Waltham, USA) and were cultured in the GIBCO Astrocyte Medium (Thermo Fisher Scientific). The glioma cell lines including SHG44, U251, U87 and SHG139 were purchased from the ATCC (Manassas, USA), and the cells were cultured in the DMEM medium with 10% fetal bovine serum (FBS; Thermo Fisher Scientific). Cells were kept in the incubator supplied with 5% carbon dioxide at 37 °C. Human astrocyte cells were used as a normal control.
Human astrocytes
Manufactured by Thermo Fisher Scientific
Sourced in United States
Human astrocytes are primary cells derived from human brain tissue. They serve as a key component of the central nervous system, providing structural and functional support to neurons.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Thermo Fisher Scientific (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Glutmax, by Thermo Fisher Scientific (2 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (2 mentions)
Tryple select, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Glucose, by Merck Group (2 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Shg 44, by American Type Culture Collection (1 mentions)
Gibco astrocyte medium, by Thermo Fisher Scientific (1 mentions)
Elisa kits for cytokines determination, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Fibroblast growth factor 2 (fgf2), by Thermo Fisher Scientific (1 mentions)
Human astrocyte, by ScienCell (1 mentions)
Dmem high glucose, by Merck Group (1 mentions)
Dmem nutrient mixture f 12 ham, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
8 protocols using human astrocytes
1
Culturing Human Astrocytes and Glioma Cells
2
Cell Viability Assay for Small Molecules
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U87 and U251 cell lines, adult human neural progenitor cells (a generous gift from Drs. Steindler & Tong, Tufts University), and human astrocytes (ThermoFisher) were seeded in a 96-well plate format with a density of 1000 cells per well and incubated for 24 h before treatment. Each well was then supplemented with fresh media containing various concentrations of 28 , ent -28 , TMZ, or vehicle control DMSO. Phase-contrast images at ×10 magnification were acquired using the IncuCyte® Live-Cell Imaging System (Essen Instruments). Each well was imaged four times every 24 h over 5 days and image-based analysis of cell morphology was carried out using the Incucyte Software. The results are representative of four independent experiments.
3
Astrocyte Culture with Gold Nanoparticles
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Gibco Human Astrocytes were obtained from Life Technologies (N7805-100, Warsaw, Poland). The cells were cultured at 37°C in Gibco Astrocyte Medium (A1261301, Dulbecco's Modified Eagle's Medium (DMEM), N-2 Supplement, and One Shot Fetal Bovine Serum (FBS)) in a humidified atmosphere in the presence of 5% CO2. According to the manufacturer's instructions, Geltrex matrix-coated plates (A14132) and 3000 cells per well in a 96-well plate or 5000 cells per well in a 4-well plate were used. Astrocytes were cultured for 24 h, medium was then discarded and fresh medium containing 1.1 × 109, 1.1 × 1010, 1.1 × 1011, and 5.5 × 1011 AuNPs/mL that corresponds to 1.4, 14, 140, and 700 ng/mL [17 (link)] was added and cells were cultured for another 96 h. Every 48 h, the medium supplemented with AuNPs was replaced by a fresh one.
4
Astrocytes Cytokine Secretion Assay
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Human astrocytes were purchased from GIBCO (Life Technologies, Milan, Italy). ELISA kits for cytokines’ determination were from Thermo Fisher Scientific, Rodano, Milan, Italy. All other reagents were obtained from standard commercial sources and were of the highest commercially available grade.
5
Culturing Human Glioblastoma and Astrocytes
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Human GBM cell lines were purchased from Sigma-Aldrich (St. Louis, MO, USA) (U251) or ATCC (Manassas, VA, USA) (U118). U251 cells were cultured in GBM culture medium, which included MEM (Gibco, Gaithersburg, MD, USA), 0.2% penicillin/streptomycin (Gibco), 10% fetal bovine serum (FBS; Gibco), 1 mM sodium pyruvate (Gibco), 1% non-essential amino acids (Gibco), and 1× GlutMAX (Gibco). U118 cells were cultured in medium containing DMEM (Gibco), 10% FBS, and 1% penicillin/streptomycin.
Human astrocytes were purchased from ScienCell (San Diego, CA, USA). Human astrocytes were cultured in human astrocyte medium, which included DMEM/F12 (Gibco), 10% FBS, 3.5 mM glucose (Sigma-Aldrich), and 0.2% penicillin/streptomycin, supplemented with B27 (Gibco), N2 (Gibco), 10 ng/mL fibroblast growth factor 2 (Invitrogen, Carlsbad, CA, USA), and 10 ng/mL epidermal growth factor (Invitrogen).
For subcultures, the cells were trypsinized using 0.25% trypsin (Gibco) or TrypLE Select (Invitrogen), centrifuged for 5 min at 800 rpm, resuspended, and plated in corresponding culture medium with a split ratio of approximately 1:4. The cells were maintained at 37 °C in humidified air with 5% CO2.
Human astrocytes were purchased from ScienCell (San Diego, CA, USA). Human astrocytes were cultured in human astrocyte medium, which included DMEM/F12 (Gibco), 10% FBS, 3.5 mM glucose (Sigma-Aldrich), and 0.2% penicillin/streptomycin, supplemented with B27 (Gibco), N2 (Gibco), 10 ng/mL fibroblast growth factor 2 (Invitrogen, Carlsbad, CA, USA), and 10 ng/mL epidermal growth factor (Invitrogen).
For subcultures, the cells were trypsinized using 0.25% trypsin (Gibco) or TrypLE Select (Invitrogen), centrifuged for 5 min at 800 rpm, resuspended, and plated in corresponding culture medium with a split ratio of approximately 1:4. The cells were maintained at 37 °C in humidified air with 5% CO2.
6
Glioblastoma Cell Lines and Patient Samples
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Human astrocytes were obtained from Gibco (Life Technologies). Human glioblastoma cell lines U251, U343, U87, LN229 and TJ905 were obtained from the Cell Bank of the Chinese Academy of Sciences or ATCC. Cells were cultured in DMEM medium with 10 % fetal bovine serum. All cells were maintained in a humidified incubator at 37°C and 5% CO2.
Tumor tissues with the corresponding paired normal tissues were obtained from glioblastoma patients at the Tianjin Nankai hospital between 2013 and 2015. Informed written consent was obtained from all patients.
Tumor tissues with the corresponding paired normal tissues were obtained from glioblastoma patients at the Tianjin Nankai hospital between 2013 and 2015. Informed written consent was obtained from all patients.
7
Culturing Human Cell Lines for Research
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All tested human types of cells were obtained from commercial sources and incubated under the recommended conditions. Briefly, human astrocytes were obtained from Gibco and grown in medium provided by a supplier. The cells in human neuroblastoma cell line SH-SY5Y (ATCC-CRL-2266) were cultured in Dulbecco's modified Eagle's medium (DMEM)/Nutrient Mixture F-12 Ham (Sigma, D8437) supplemented with 10% (v/v) fetal bovine serum (FBS), penicillin (100 U/ml) and streptomycin sulphate (0.1 mg/ml). Cells in human glioblastoma cell line A172 (ATCC-CRL-1620) and astrocytoma cell line SW1088 (ATCC-HTB-12) were cultured in DMEM-high glucose (Sigma, D6429) supplemented with 10% (v/v) FBS, penicillin (100 U/ml) and streptomycin sulphate (0.1 mg/ml).
The cells were kept in a humidified incubator in an atmosphere enriched to 5% CO 2 at 37°C. The plastic culture wells and dishes were obtained from Sigma.
The cells were kept in a humidified incubator in an atmosphere enriched to 5% CO 2 at 37°C. The plastic culture wells and dishes were obtained from Sigma.
8
Culturing Human Glioblastoma and Astrocyte Cells
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Human GBM cell lines were purchased from Sigma (U251) or ATCC (U118). U251 cells were cultured in GBM culture medium, which included MEM (GIBCO), 0.2% penicillin/streptomycin (GIBCO), 10% FBS (GIBCO), 1mM Sodium Pyruvate (GIBCO), 1% Non Essential Amino Acids (NEAA, GIBCO), and 1 x GlutMAX (GIBCO). U118 cells were cultured in culture medium including DMEM (GIBCO), 10% FBS and 1% penicillin/streptomycin. Human astrocytes were purchased from ScienCell (HA1800, San Diego, USA).
Human astrocytes were cultured in human astrocyte medium, which included DMEM/F12 (GIBCO), 10% FBS, 3.5 mM Glucose (Sigma), and 0.2% penicillin/streptomycin, supplemented with B27 (GIBCO), N2 (GIBCO), 10 ng/ml fibroblast growth factor 2 (FGF2, Invitogen), and 10 ng/ml epidermal growth factor (EFG, Invitrogen).
For subculture, cells were Trypsinized by 0.25% Trypsin (GIBCO) or TrypLE Select (Invitrogen), centrifuged for 5 min at 800 rpm, re-suspended and plated in corresponding culture medium with a split ratio around 1:4. Cells were maintained at 37°C in humidified air with 5% CO 2 .
Human astrocytes were cultured in human astrocyte medium, which included DMEM/F12 (GIBCO), 10% FBS, 3.5 mM Glucose (Sigma), and 0.2% penicillin/streptomycin, supplemented with B27 (GIBCO), N2 (GIBCO), 10 ng/ml fibroblast growth factor 2 (FGF2, Invitogen), and 10 ng/ml epidermal growth factor (EFG, Invitrogen).
For subculture, cells were Trypsinized by 0.25% Trypsin (GIBCO) or TrypLE Select (Invitrogen), centrifuged for 5 min at 800 rpm, re-suspended and plated in corresponding culture medium with a split ratio around 1:4. Cells were maintained at 37°C in humidified air with 5% CO 2 .
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