DMEM (Dulbecco’s modified eagle medium) powder was procured from Gibco by Life Technology (Thermo Fisher Scientific, Waltham, MA, USA). Sodium bicarbonate (NaHCO3), calcium chloride (CaCl2.2H2O), and alpha-ketoglutaric acid disodium salt hydrate salts were purchased from Sigma-Aldrich (St Louis, MO, USA). Anti-cancer drug AZ628, DMSO (Dimethyl sulphoxide) and thiazolyl blue tetrazolium bromide (MTT) were obtained from Sigma–Aldrich (St Louis, MO, USA). DMEM-high glucose liquid media, FBS (Fetal Bovine Serum), TrypLE Express and penicillin-streptomycin were acquired from Sigma–Aldrich (St Louis, MO, USA). Acetonitrile (ACN) was from Fischer Scientific (Loughborough, UK). Bradford 1× dye reagent was purchased from Bio-rad Laboratories (Hercules, CA, USA).
Calcium chloride cacl2.2h2o
Manufactured by Merck Group
Sourced in United States
Calcium chloride (CaCl2.2H2O) is a laboratory chemical compound. It is the dihydrate form of calcium chloride, consisting of calcium, chlorine, and water molecules. Calcium chloride is commonly used in various laboratory applications due to its chemical properties.
Automatically generated - may contain errors
Lab products found in correlation
Bradford 1 dye reagent, by Bio-Rad (1 mentions)
Sodium bicarbonate nahco3, by Merck Group (1 mentions)
Az628, by Merck Group (1 mentions)
Acetonitrile acn, by Thermo Fisher Scientific (1 mentions)
Dmso dimethylsulphoxide, by Merck Group (1 mentions)
Thiazolyl blue tetrazolium bromide mtt, by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Paraquat pq, by Merck Group (1 mentions)
2 4 dichlorophenoxyacetic acid, by Merck Group (1 mentions)
Absolute ethanol, by Merck Group (1 mentions)
Ludox hs 40, by Merck Group (1 mentions)
Polyoxyethylene sorbitan monooleate, by Merck Group (1 mentions)
Span 80, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
3 protocols using calcium chloride cacl2.2h2o
1
Cell Viability Assay with AZ628 Drug
2
Characterization of Herbicide Contaminants
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Metsulfuron methyl, MM (CAS: 74223-64-6, 99.3% purity, pKa: 3.3), 2,4-dichlorophenoxyacetic acid, 2,4-D (CAS: 94-75-7, 99.3% purity, pKa: 2.98) and paraquat, PQ (CAS: 75365-73-0, 98.0% purity) were purchased from Riedel-de Haën, Sigma-Aldrich. The 10 mM stock solutions at pH 6.0 ± 0.2 were prepared in 0.01 M NaCl. pH 6.0 ± 0.2 was adjust for all solutions using small volumes of HCl and/or NaOH solutions (1 M). pH measurements were performed by using a Cole Parmer Model 59003-25 pH meter with an Alpha® model PY-41 electrode.
The molecular structures of all contaminants are shown in Table S1 of the ESI† . Considering the pKa values of 2,4-D and MM their anionic species are predominant at working pH; on the other hand, BAC-12, BAC-14 and PQ are quaternary ammonium salts, therefore they only form cations in aqueous solution.44 Calcium chloride CaCl2·2H2O (CAS: 10035-04-8, 99.0% purity) was supplied by Sigma-Aldrich Company. All other chemicals were analytical grade.
The molecular structures of all contaminants are shown in Table S1 of the ESI
3
Synthesis of Magnetic Silica Nanoparticles
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The oil phase was composed of sunflower oil (Sainsburys) and different concentrations of sorbitan monooleate (Span 80, Sigma-Aldrich). The aqueous phase was composed of deionized water, ludox HS40 (40 wt% silica suspension in H2O, diameter of 23 nm, Sigma-Aldrich), iron oxide (Fe3O4, dry powder, diameter of 50-100 nm, 97%, Alfa Aesar) and polyoxyethylene sorbitan monooleate (Tween 80, Merck). The pH was between 9 and 10. In this range, both silica and iron oxide nanoparticles are negatively charged, exhibiting a zeta potential of magnitude greater than -30 mV. Sodium chloride (NaCl, Sigma-Aldrich) and calcium chloride (CaCl2.2H2O, Sigma-Aldrich) were used to induce a colloidal instability.
Absolute ethanol (98% v/v, Sigma-Aldrich) and polyoxyethylenesorbitan monolaurate (Tween 20, Aqueous organics) were used during the cleaning and redispersion stages. All the chemicals were employed as received without additional purification.
Absolute ethanol (98% v/v, Sigma-Aldrich) and polyoxyethylenesorbitan monolaurate (Tween 20, Aqueous organics) were used during the cleaning and redispersion stages. All the chemicals were employed as received without additional purification.
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