7.0 tesla superconducting magnet

UV IKON 940 spectrophotometer was used to measure UV spectra. A Bruker Apex III Fourier-transform ion cyclotron resonance (FTICR) mass spectrometer (Bruker Daltonics, Billerica, USA) including an Infinity™ cell and a 7.0 Tesla superconducting magnet (Bruker, Karlsruhe, Germany) was used to perform mass spectrometric studies. A Bruker DRX-600 MHz Ultrashield spectrometer (Bruker BioSpin, Billerica, MA, USA) was utilized to measure NMR spectra. Chromatographic separation of the active compounds was performed on Silica gel 60 (70-230 mesh, Merck, Darmstadt, Germany), Silica gel 100 C₁₈-Reversed phase (0.04–0.063 mm, Merck, Darmstadt, Germany), and Sephadex LH-20 (Pharmacia Fine Chemicals Inc., Uppsala, Sweden). Monitoring of the isolation process was carried out on TLC plates with Silica gel 60 F₂₅₄ (Merck, Darmstadt, Germany).

The high resolution ESI mass spectra of the extracts were obtained from a Bruker Apex III Fourier transform ion cyclotron resonance (FTICR) mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with an Infinity™ cell, a 7.0 Tesla superconducting magnet (Bruker, Karlsruhe, Germany), an RF-only hexapole ion guide and an external APOLLO electrospray ion source (Agilent, off axis spray, voltages: endplate, 3.700 V; capillary, −4.200 V; capillary exit, 100 V; skimmer 1, 15.0 V; skimmer 2, 10.0 V). Nitrogen was used as drying gas at 150 °C. The sample solutions were introduced continuously via a syringe pump with a flow rate of 120 μL·h⁻¹. All data were acquired with 512 k data points and zero filled to 2048 k by averaging 32 scans. The XMASS Software (Bruker, Version 6.1.2) was used for evaluating the data.