Methyllycaconitine mla

Manufactured by Abcam

Sourced in United Kingdom

Methyllycaconitine (MLA) is a natural product compound that functions as a potent and selective antagonist of the alpha7 nicotinic acetylcholine receptor. It is commonly used in scientific research to study the role of the alpha7 nicotinic receptor in various biological processes.

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Lab products found in correlation

Pnu 120596, by Selleck Chemicals (1 mentions) Sb366791, by Merck Group (1 mentions) Mouse syt1, by Synaptic Systems (1 mentions) Neurotrace, by Thermo Fisher Scientific (1 mentions) Capsaicin, by Merck Group (1 mentions) Scopolamine hydrobromide, by Merck Group (1 mentions) Bicinchoninic acid assay kit, by Thermo Fisher Scientific (1 mentions) Nicotine hydrogen tartrate, by Merck Group (1 mentions) Cell lysis buffer, by Cell Signaling Technology (1 mentions) Sodium chloride (nacl), by Baxter (1 mentions) Phenylmethanesulfonyl fluoride pmsf, by Santa Cruz Biotechnology (1 mentions) Precast polyacrylamide gel, by Thermo Fisher Scientific (1 mentions) Polyvinylidene fluoride pvdf membrane, by Thermo Fisher Scientific (1 mentions) Ethylene diamine tetraacetic acid (edta), by Boston BioProducts (1 mentions) Ethylene glycol tetraacetic acid (egta), by Boston BioProducts (1 mentions) Tween 20, by Merck Group (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Tween 80, by Merck Group (1 mentions) Sodium orthovanadate, by Merck Group (1 mentions)

5 protocols using methyllycaconitine mla

Neurochemical Reagents for Cell Studies

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PNU-120596 was purchased from Selleck Chemicals (Houston, TX, USA). Methyllycaconitine (MLA) was purchased from Abcam Biochemicals (Cambridge, MA, USA). KN92/KN93 were purchased from Cayman Chemical (Ann Arbor, MI, USA). Other chemicals were purchased from ThermoFischer Scientific (Waltham, MA, USA).

Sun F., Johnson S.R., Jin K, & Uteshev V.V. (2016). Boosting Endogenous Resistance of Brain to Ischemia. Molecular neurobiology, 54(3), 2045-2059.

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Immunofluorescent Labeling of Neuronal Markers

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Primary antibodies used were: chick and rabbit EGFP (1:4,000 dilution; Abcam), mouse GAD65 (1:1,000), guinea pig (1:800 for uptake) and mouse syt1 (1:150 for uptake), rabbit alpha tubulin (1:5,000), mouse syb2 (1:1,000 dilution; Synaptic Systems), chick and mouse MAP2 (1:4,000 dilution; Millipore), sheep NGF, mouse reelin, guinea pig TRPV1 C-T (1:1,000, Millipore), mouse TRPV1 N-T (1:500; Neuromab), rabbit TRPV2, TRPV3, TRPV4 (1:500; Alomone), rabbit mGluR7 (1:500; Upstate), mouse PV25 (1:2,000 dilution; Swant), rabbit Somatostatin-14 (1:2,000 dilution; Bachem), rat Somatostatin-14 (1:1,000 dilution; Thermo Fisher), rabbit VIP (1:500 dilution; Abcam), mouse BDNF (1:500; Developmental Studies Hybridoma Bank; Yves Barde monoclonal #9). Secondary antibodies used were: guinea pig, mouse and rabbit HRP (1:5,000 dilution; BioRad) and chick, rabbit, guinea pig, mouse, rat and sheep Alexa 405, 488, 546 and 647 (1:2,000 dilution; Abcam/Invitrogen). Neurotrace was from Thermo Fisher.
Capsaicin and SB-366791 were purchased from Sigma-Aldrich. Methyllycaconitine (MLA) was from Abcam and dihydro-beta-erythroidine (DHBE), mecamylamine (MEC) picrotoxin, TTX, APV and CNQX were from Tocris. Recombinant Human TrkA Fc and TrkB Fc Chimera Proteins were from R & D Systems. All other reagents were from Carl Roth.

Hurtado-Zavala J.I., Ramachandran B., Ahmed S., Halder R., Bolleyer C., Awasthi A., Stahlberg M.A., Wagener R.J., Anderson K., Drenan R.M., Lester H.A., Miwa J.M., Staiger J.F., Fischer A, & Dean C. (2017). TRPV1 regulates excitatory innervation of OLM neurons in the hippocampus. Nature Communications, 8, 15878.

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Nicotinic Receptor Signaling Pathway Protocol

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(-)-Nicotine hydrogen tartrate, scopolamine hydrobromide, (2.4)-dimethoxybenzylidene anabaseine dihydrochloride (DMXBA, also called GTS-21), sodium orthovanadate, protease inhibitor cocktail, Tween 20, Tween 80, were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium chloride (NaCl) was purchased from Baxter (Lublin, Poland). Methyllycaconitine (MLA), and antibodies for the α7 nAChR (#ab23832) and β-actin were obtained from Abcam (Cambridge, UK). Antibodies detecting phospho-ERK1/2 (#4376, Thr202/Tyr204) and total ERK1/2 (#4695) were purchased from Cell Signaling Technology (Danvers, MA, USA). EDTA and EGTA were purchased from Boston BioProducts (Ashland, MA, USA). Phenylmethanesulfonyl fluoride (PMSF) was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Phosphatase inhibitor cocktail sets I and II were purchased from Calbiochem (San Diego, CA, USA). The bicinchoninic acid assay kit was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Polyvinylidene fluoride (PVDF) membranes and 4-12% pre cast polyacrylamide gels were purchased from Invitrogen (Carlsbad, CA, USA). Horseradish peroxidase-conjugated secondary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and the Cell Lysis Buffer was obtained from Cell Signaling Technology (MA, USA). PAM-2 was synthesized as described in Arias et al [8] .

Targowska-Duda K.M., Wnorowski A., Budzynska B., Jozwiak K., Biala G, & Arias H.R. (2016). The positive allosteric modulator of α7 nicotinic acetylcholine receptors, 3-furan-2-yl-N-p-tolyl-acrylamide, enhances memory processes and stimulates ERK1/2 phosphorylation in mice. Behavioural brain research, 302.

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Investigating α7nAChR Modulation in Neurotherapy

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PHA 568487 (PHA, an α7nAChR agonist) was purchased from Santa Cruz Biotechnology (sc-204186; Dallas, TX, United States). Methyllycaconitine (MLA, an α7nAChR antagonist) was purchased from Abcam (ab146684; Cambridge, United Kingdom). PHA and MLA were dissolved in 0.9% sterile saline and administered intraperitoneally (i.p.). K-252a (an antagonist of the TrkB receptor) was purchased from Sigma-Aldrich (K2015; St. Louis, MO, United States). The control group received the same volume of saline.

Wei P., Lyu W., Xu L., Feng H., Zhou H, & Li J. (2022). α7 Nicotinic Acetylcholine Receptor May Be a Pharmacological Target for Perioperative Neurocognitive Disorders. Frontiers in Pharmacology, 13, 907713.

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Pharmacological Modulation of Neuronal Receptors

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Methyllycaconitine (MLA), donepezil, TTX and 6,7-dinitroquinoxaline-2,3-dione (DNQX) were purchased from Abcam. PNU-120596 and PNU-282987 were provided by Pfizer Inc. USA. All other drugs and compounds were purchased from Sigma Aldrich.

Udakis M., Wright V.L., Wonnacott S, & Bailey C.P. (2016). Integration of inhibitory and excitatory effects of α7 nicotinic acetylcholine receptor activation in the prelimbic cortex regulates network activity and plasticity. Neuropharmacology, 105, 618-629.

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