Sal003

Manufactured by Bio-Techne

Sourced in United States, Japan

Sal003 is a laboratory instrument designed for cell culture experiments. It provides precise temperature and environmental control to support the growth and maintenance of cells in vitro.

Automatically generated - may contain errors

Lab products found in correlation

Cycloheximide (chx), by Merck Group (2 mentions) Nicotine hydrogen tartrate, by Merck Group (1 mentions) Cocaine hydrochloride, by Merck Group (1 mentions) Polyinosinic polycytidylic acid, by InvivoGen (1 mentions) Isrib, by Bio-Techne (1 mentions) Brefeldin a, by Bio-Techne (1 mentions) Golgicide a, by Bio-Techne (1 mentions) Tunicamycin, by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) Nocodazole, by Bio-Techne (1 mentions) Guanabenz, by Merck Group (1 mentions) Trcn0000020239, by Merck Group (1 mentions) Sorbitol, by Merck Group (1 mentions) Torin1, by Bio-Techne (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin glutamine (psg), by Thermo Fisher Scientific (1 mentions) Harringtonine, by LKT Laboratories (1 mentions) Sephin1, by Apexbio (1 mentions)

4 protocols using sal003

Pharmacological Modulation of Cocaine Effects

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All drugs of abuse were dissolved in 0.9% saline and injected in a volume of 5 ml/kg. Cocaine hydrochloride, (−)-nicotine hydrogen tartrate, and USP-grade 95% ethanol were obtained from Sigma-Aldrich (St. Louis, MO). Racemic methamphetamine hydrochloride was a kind gift from Dr. Kristen Horner (Mercer University School of Medicine). ISRIB (P. Walter) was dissolved in DMSO and further diluted in PEG-400 (1:1 ratio) as previously described (Di Prisco et al., 2014 (link)). For both electrophysiological and behavioral experiments, ISRIB (2.5 mg/kg) or vehicle (DMSO/PEG-400, 2 ml/kg) was injected 90 min before cocaine or saline injection, respectively. Sal003 (Tocris Biosciences, R&D Systems, Minneapolis, MN) was dissolved in DMSO and further diluted in 0.9% saline. Sal003 (20 μM) or vehicle (0.4% DMSO in saline) was infused bilaterally into the VTA as summarized in the 'Cannulation and Sal003 infusion' section.

Huang W., Placzek A.N., Viana Di Prisco G., Khatiwada S., Sidrauski C., Krnjević K., Walter P., Dani J.A, & Costa-Mattioli M. (2016). Translational control by eIF2α phosphorylation regulates vulnerability to the synaptic and behavioral effects of cocaine. eLife, 5, e12052.

+ Open protocol

+ Expand

Modulating Integrated Stress Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tunicamycin (TUN; #12819S; Cell Signaling Technology, Danvers MA, USA) and SAL003 (SAL; #3657; Tocris Bioscience, Bristol UK) were used as generalized ISR activators. Halofuginone (HF; Selleck Chemicals, Houston, TX, USA), sodium arsenite (ARS; MilliporeSigma, St. Louis, MO, USA), and polyinosinic:polycytidylic acid (poly I:C; InvivoGen, San Diego, CA, USA) were applied as GCN2, HRI, PKR, and PERK agonists to induce specific arms of ISR, respectively. Trans-integrated stress response inhibitor (trans-ISRIB; Tocris Bioscience) was investigated as an ISR inhibitor.

Chu H.S., Peterson C., Chamling X., Berlinicke C., Zack D., Jun A.S, & Foster J. (2022). Integrated Stress Response Regulation of Corneal Epithelial Cell Motility and Cytokine Production. Investigative Ophthalmology & Visual Science, 63(8), 1.

+ Open protocol

+ Expand

Modulating SNAT2, GORASP2 and GADD34 in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Propagation of lentiviral particles expressing shRNA against human SNAT2, GORASP2 and human and mouse GADD34 (TRCN0000020239, TRCN0000278406, TRCN0000003041 and TRCN0000353349, respectively; Sigma-Aldrich) or empty vector (pLKO.1) was performed in HEK293T cells. The osmolarity of media was increased by addition of NaCl for corneal epithelial cells or sorbitol for MEFs. The following reagents were purchased from commercial vendors: Sal003, nocodazole, ISRIB, brefeldin A and golgicide A (Tocris); cycloheximide, MG132, guanabenz and tunicamycin (Sigma-Aldrich). Sephin 1 was a generous gift from Anne Bertolotti (University of Cambridge, UK).

Krokowski D., Guan B.J., Wu J., Zheng Y., Pattabiraman P.P., Jobava R., Gao X.H., Di X.J., Snider M.D., Mu T.W., Liu S., Storrie B., Pearlman E., Blumental-Perry A, & Hatzoglou M. (2017). GADD34 function in protein trafficking promotes adaptation to hyperosmotic stress in human corneal cells. Cell reports, 21(10), 2895-2910.

+ Open protocol

+ Expand

Induction of Cellular Stress Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

The MEFs used were described previously (Scheuner et al., 2001 (link)). The cells were grown in high glucose Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, 11960044) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, 26140079), 1X Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific, 10378016) at 37°C and 5% CO₂. For all experiments, cells were subcultured 24 hours prior to the experiment so that cells were ~80% confluent at the time of the experiment. GADD34^−/− MEFs were a gift from D. Ron (Novoa et al., 2003 (link)). Hyperosmotic stress was induced with Sorbitol (Sigma-Aldrich, S1876). Other chemicals used in this study include Cycloheximide (CHX) (100 μg/mL) (Sigma-Aldrich C7698), Harringtonine (2 μg/mL) (LKT Laboratories, H0169), Sephin1 (Apexbio, A8708), Sal 003 (1 μM) (Tocris Bioscience, 3657), Torin 1 (250 nM) (Tocris Bioscience, 4247), Hippuristanol (1 μM) (a generous gift from Dr. Junichi Tanaka, University of the Ryukyus, Japan) (Bordeleau et al., 2006 (link)) and CCCP (10 μM) (Sigma-Aldrich, C2759).

Jobava R., Mao Y., Guan B.J., Hu D., Krokowski D., Chen C.W., Shu X.E., Chukwurah E., Wu J., Gao Z., Zagore L.L., Merrick W.C., Trifunovic A., Hsieh A.C., Valadkhan S., Zhang Y., Qi X., Jankowsky E., Topisirovic I., Licatalosi D.D., Qian S.B, & Hatzoglou M. (2021). Adaptive translational pausing is a hallmark of the cellular response to severe environmental stress. Molecular cell, 81(20), 4191-4208.e8.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!