Interleukin 6 (il 6)

Manufactured by RnD Systems

Sourced in United States

IL-6 is a cytokine that plays a crucial role in the immune system. It is involved in the regulation of various cellular processes, including inflammation, immune response, and hematopoiesis. This lab equipment product provides researchers with a reliable tool to study the functional aspects of IL-6 in their research applications.

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Lab products found in correlation

Lenalidomide, by Selleck Chemicals (1 mentions) Attune acoustic focusing cytometer, by Thermo Fisher Scientific (1 mentions) Celltracker red cmptx dye, by Thermo Fisher Scientific (1 mentions) Attune software, by Thermo Fisher Scientific (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Stemline 2 media, by Merck Group (1 mentions) G csf, by RnD Systems (1 mentions) Interleukin 3 (il 3), by RnD Systems (1 mentions) Hibit lytic reagent, by Promega (1 mentions) Gm csf, by RnD Systems (1 mentions) Celltiter glo reagent, by Promega (1 mentions) Il 1β, by RnD Systems (1 mentions) Il 18, by MBL Life Science (1 mentions)

5 protocols using interleukin 6 (il 6)

Investigating IL-6 Effects on Cancer Cells

Cited 1 time

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SCC-25/Detroit 562 cells were treated with 7 ml EMC-CM per 50 ml cell culture flask for 72 h. The medium was changed daily. To assess the effects of IL-6 SCC-25/ Detroit 562 cells were cultivated in albumin-containing medium supplemented with IL-6 at 50 ng/ml (RnD Systems, Biomedica, Vienna, Austria). The concentration of IL-6 was based on pre-experiments and on a publication of Sullivan and colleagues [29 (link)]. Exposure conditions were the same as in the first experimental group, i.e., IL-6 supplemented medium was used over a period of 72 h. At the end of the stimulation period, cells were used for immunohistochemistry, protein isolation and MTT assays respectively.

Steinbichler T.B., Savic D., Dejaco D., Romani A., Kofler B., Skvortsova I.I., Riechelmann H, & Dudas J. (2019). Pleiotropic Effects of Epithelial Mesenchymal Crosstalk on Head and Neck Cancer: EMT and beyond. Cancer Microenvironment, 12(2-3), 67-76.

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Evaluating Combination Therapies on Myeloma Cells

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Myeloma cell lines (5×10⁴ per well) were incubated with CPI203 (kindly provided by Constellation Pharmaceuticals, Cambridge, MA, USA) and/or lenalidomide (Selleck Chemicals LLC, Houston, TX, USA) plus dexamethasone (Merck, S.L., Darmstadt, Germany) at indicated doses in triplicates. MTT [3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide] assay (Sigma-Aldrich, St Louis, MO, USA) was used to evaluate the effect of the drugs on cell proliferation.
Primary cells were labeled with CellTracker™ Red CMPTX dye (Thermo Fisher) following the manufacturer’s protocol and co-cultured with the mesenchymal stromal cell line stromaNKtert in the presence of 10 ng/mL IL-6 (RnD Systems, Minneapolis, MN, USA). Cell proliferation was analyzed in an Attune acoustic focusing cytometer using Attune software (Thermo Fisher).

Díaz T., Rodríguez V., Lozano E., Mena M.P., Calderón M., Rosiñol L., Martínez A., Tovar N., Pérez-Galán P., Bladé J., Roué G, & de Larrea C.F. (2017). The BET bromodomain inhibitor CPI203 improves lenalidomide and dexamethasone activity in in vitro and in vivo models of multiple myeloma by blockade of Ikaros and MYC signaling. Haematologica, 102(10), 1776-1784.

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Dose-dependent cytokine treatment of hTSCs

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hTSCs were seeded at 40,000 cells per well in a 24-well plate and incubated overnight at 37 °C in 8% oxygen. Cells were treated (triplicate, n = 5) for 24 h with 0, 0.1, 1 and 10 ng/mL doses of TNFα (Life Technologies, Carlsbad, USA), IL-6 (RnD systems, Minnesota, USA), or vehicle control (sterilised water or dPBS respectively). Following treatment, cell lysates were collected for RNA extraction and qRT-PCR.

Baird L., Cannon P., Kandel M., Nguyen T.V., Nguyen A., Wong G., Murphy C., Brownfoot F.C., Kadife E., Hannan N.J., Tong S., Bartho L.A, & Kaitu’u-Lino T.J. (2023). Paternal Expressed Gene 10 (PEG10) is decreased in early-onset preeclampsia. Reproductive Biology and Endocrinology : RB&E, 21, 65.

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High-Throughput Cytokine-Driven BMDC Culture

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Per well, 6000 BMDCs in 15 µl of media containing Stemline II media (Sigma, catalog no. S0192-6X500ML), 10% FBS (ThermoFisher, catalog no. 26140095) penicillin-streptomycin (Fisher, catalog no. MT30002CI), and cytokines (50 ng/ml SCF, RnD Systems, catalog no. 455-MC-050; 20 ng/ml G-CSF, RnD Systems, catalog no. 414-CS-025; 20 ng/ml GM-CSF, RnD Systems, catalog no. 415-ML-020; 50 ng/ml FLT3, RnD Systems, catalog no. 768-F3-050; 20 ng/ml IL-6, RnD Systems, catalog no. 406-ML-200; 10 ng/ml IL-3, RnD Systems, catalog no. 403-ML-050; 30 ng/ml TPO, RnD Systems, catalog no. 488-TO-200; 1.5 ng/ml EPO, RnD Systems, catalog no. 959-ME-010) were placed into a black-walled 384-well plate (Corning, catalog no. 3764) and left overnight. Next day, 15 µl of 2× drug-containing solution was added to each well. For HiBit assays, 6 h later the HiBit Lytic reagent was added following the manufacturer’s protocols (Promega, catalog no. N3030) and plates were read using a Biotek H1 Synergy H1 Hybrid plate reader. For viability assays, 4 days after compound addition, the CellTiter-Glo reagent was added following the manufacturer’s protocols (Promega, catalog no. G7573) and plates were read using a Biotek H1 Synergy H1 Hybrid plate reader.

Yenerall P., Sung T., Palyada K., Qian J., Arat S., Kumpf S.W., Wang S.W., Biddle K., Esparza C., Chang S., Scott W., Collette W., Winrow T.S., Affolter T., Shirai N., Thibault S., Wang J., Liu L., Bauchmann M., Frey J., Steyn S., Sacaan A., Vitsky A., Ahn Y., Paul T., Lum L., Oyer J., Yang A, & Hu W. (2023). Use of the dTAG system in vivo to degrade CDK2 and CDK5 in adult mice and explore potential safety liabilities. Toxicological Sciences, 194(1), 53-69.

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Inflammasome Marker Quantification by ELISA

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Changes in cellular or excreted protein levels were measured by enzyme-linked immunosorbent assay (ELISA), to determine the expression of key inflammasome related markers including: IL-18 (MBL International, Woburn, USA), IL-1β, IL-6 and pro-IL-1β (RnD systems, Minneapolis, USA) and caspase-1 (Cusabio, Wuhan, China). Importantly, the antibodies used in IL-1β and pro-IL-1β ELISA are specific to each antigen. Results from cell lysates were normalised to total protein concentration as determined by a BCA assay.

Robertson S., Martínez G.J., Payet C.A., Barraclough J.Y., Celermajer D.S., Bursill C, & Patel S. (2016). Colchicine therapy in acute coronary syndrome patients acts on caspase-1 to suppress NLRP3 inflammasome monocyte activation. Clinical science (London, England : 1979), 130(14).

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