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Mef2 luc

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The MEF2-luc is a reporter gene construct that contains the Firefly luciferase gene under the control of the Myocyte Enhancer Factor 2 (MEF2) promoter. The MEF2 transcription factor is involved in the regulation of genes related to muscle development and function.

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Lab products found in correlation

Nfat luc, by Promega (2 mentions) Creb1 luc, by Promega (2 mentions) Gata4 luc, by Promega (2 mentions) Tk luc, by Promega (2 mentions) Veritas 96 well microplate luminometer, by Promega (2 mentions) Xfect reagent, by Takara Bio (2 mentions) Dual luciferase assay kit, by Promega (2 mentions) Passive lysis buffer (plb), by Promega (2 mentions)

2 protocols using mef2 luc

1

Transcription Factor Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNCMs were transfected with NFAT-luc, CREB1-luc, GATA4-luc, TK-luc (Promega) or MEF2-luc (Addgene), and using X-fect reagent (Clontech) according to manufacturer’s protocol. After 24 hours transfection, cells were stimulated with 25 μM of PE for 6 hours, lysates harvested using passive lysis buffer (Promega) and luciferase activity determined using the Dual Luciferase assay kit (Promega) and Veritas 96 well microplate luminometer (Turner Biosystems) following manufacturer’s protocol.
Lee D.I., Zhu G., Sasaki T., Cho G.S., Hamdani N., Holewinski R., Jo S.H., Danner T., Zhang M., Rainer P.P., Bedja D., Kirk J.A., Ranek M.J., Dostmann W.R., Kwon C., Margulies K.B., Van Eyk J.E., Paulus W.J., Takimoto E, & Kass D.A. (2015). Phosphodiesterase 9A Controls Nitric-oxide Independent cGMP and Hypertrophic Heart Disease. Nature, 519(7544), 472-476.
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2

Transcription Factor Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNCMs were transfected with NFAT-luc, CREB1-luc, GATA4-luc, TK-luc (Promega) or MEF2-luc (Addgene), and using X-fect reagent (Clontech) according to manufacturer’s protocol. After 24 hours transfection, cells were stimulated with 25 μM of PE for 6 hours, lysates harvested using passive lysis buffer (Promega) and luciferase activity determined using the Dual Luciferase assay kit (Promega) and Veritas 96 well microplate luminometer (Turner Biosystems) following manufacturer’s protocol.
Lee D.I., Zhu G., Sasaki T., Cho G.S., Hamdani N., Holewinski R., Jo S.H., Danner T., Zhang M., Rainer P.P., Bedja D., Kirk J.A., Ranek M.J., Dostmann W.R., Kwon C., Margulies K.B., Van Eyk J.E., Paulus W.J., Takimoto E, & Kass D.A. (2015). Phosphodiesterase 9A Controls Nitric-oxide Independent cGMP and Hypertrophic Heart Disease. Nature, 519(7544), 472-476.
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