Fluorescein isothiocyanate fitc labeled goat anti mouse igg

F81, Hela, and BHK-21 cells, which were purchased from China Center for Type Culture Collection (Wuhan, China), were cultured in minimum essential media containing 10% (v/v) fetal bovine serum and 1% (w/v) penicillin—streptomycin at 37°C and 5% CO₂.
3-MPA was purchased from Sigma-Aldrich. QDs (shell/core ZnS/CdSe) were a production of Wuhan Jiayuan Quantum Dot Co. LTD (China). 1-Ethyl-3-(3-dimethlyaminopropyl) carbodiimide hydrochloride, N-hydroxysuccinimide were purchased from Sigma-Aldrich. Fluorescein Isothiocyanate (FITC)-labeled goat anti-mouse IgG was purchased from Sigma-Aldrich.

Frozen sections of rat eyes from the intracameral injection group were used for immunofluorescence staining of α-smooth muscle actin (α-SMA) to localize ciliary muscle. They were fixed in 4% paraformaldehyde for 10 min and blocked with normal goat serum at room temperature for 1 h. This was followed by incubation with anti-α-SMA mouse monoclonal antibody (1 : 100) (Sigma-Aldrich, St. Louis, USA) at 4°C overnight and subsequent incubation with fluorescein isothiocyanate- (FITC-) labeled goat anti-mouse IgG (1 : 50) (Sigma-Aldrich, St. Louis, USA) at room temperature for 2 h. Finally, the sections were incubated with DAPI nuclear staining solution at room temperature for 5 min, mounted with an antifluorescent quenching agent (Beyotime Institute of Biotechnology, Shanghai, China) and observed under a fluorescence microscope.