MRI data for infarcted hearts were acquired using the Bruker 7T Biospec 70/30 USR System (Bruker, Billerica, MA, USA) and a small animal volume coil (RF RES 300 1H 075/040 quadrature surface transmit receive coil, Bruker). We anesthetized the mouse with 2–3% isoflurane in room air and acquired ECG, respiration, and temperature data using the MR-Compatible Monitoring and Gating System (SA Instruments, Stony Brook, NY, USA). ECG and respiratory-gated cine fast low angle shot (FLASH) pulse sequence (TE = 3 ms; TR = 7 ms; FA = 20°; Matrix Size = 256 × 256; Number of averages = 1) was used to acquire contiguous 1 mm thick short-axis cine loops of a beating left ventricle.
Mr compatible monitoring and gating system
Manufactured by SA Instruments
Sourced in United States
The MR compatible monitoring and gating system is a device designed to provide vital sign monitoring and gating functionality for use in magnetic resonance (MR) imaging environments. The system is engineered to be compatible with MR imaging equipment and facilitates the simultaneous collection of physiological data during MR scans.
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Lab products found in correlation
5 protocols using mr compatible monitoring and gating system
1
Cardiac MRI of Mouse Infarction
2
Magnetic Particle Tracking in Rat Brainstem
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To determine the location and fate of the magnetic particles once they were injected into the brainstem of rats, MRI was performed on the 9.4 T MRI scanner with a 72 mm inner diameter volume coil (RAPID Biomedical) for radiofrequency transmission and a 4‐channel array head coil (RAPID Biomedical) for signal reception. Anesthesia was induced with 4% isoflurane and maintained with 1.5% isoflurane. Body temperature was maintained with a heated waterbed. Respiratory rate and body temperature were monitored with a pneumatic pillow sensor and a rectal thermister probe respectively, both connected to a MR‐compatible monitoring and gating system (SA Instruments). A T2*‐weighted gradient‐echo sequence was used: TE = 6.5 ms, TR = 2230 ms, flip angle = 56°, number of averages = 5, FOV = 28.8 mm × 28.8 mm, matrix = 192 × 192, slice thickness = 0.15 mm, interslice distance = 0 mm. The images have an isotropic resolution of 150 µm.
Affine registration of the MR images was performed using the NifTK software.[71 ]
Affine registration of the MR images was performed using the NifTK software.[
3
Preclinical MR Imaging of Mice
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MR experiments were performed on a preclinical 7T scanner (PharmaScan, Bruker, Ettlingen, Germany). A 72 mm body volume coil (Bruker, Ettlingen, Germany) and a four-channel phased array receive-only MRI CryoProbe (Bruker, Ettlingen, Germany) were used for excitation and reception, respectively. Mice were maintained under anesthesia during MR experiments by spontaneous respiration of a mixture of air and isoflurane (1.5%, constant flow, 300 mL/min; Univentor 400 anesthesia unit, Zejtun, Malta) through a dedicated nose cone. Ear bars were used to limit the mice head movement. Respiration and temperature were controlled throughout the experiments with an MR-compatible monitoring and gating system (SA Instruments, Stony Brook, NY, USA). A heating blanket connected to a water bath system was placed under the mice to carefully maintain a temperature of 37.5 ± 0.5 °C.
4
In vivo MRI Imaging of Mice
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Animal studies were conducted in agreement with the French guidelines for animal care from the French Department of Agriculture (Animal Rights Division), the European Council Directive 86/ 609/EEC of 24 November 1986, and approved by our institutional committee on Ethics in animal research. After isoflurane induction in an anesthetic chamber at 3%, healthy female C57Bl/6J mice (10 weeks, 21 6 2 g) were maintained anaesthetized by spontaneous respiration of a mixture of air and isoflurane (1.5%, constant flow 300 mL/min, Univentor 400 anesthesia unit, Zejtun, Malta), through a dedicated nose cone. Respiration was controlled throughout the experiment with a MR compatible monitoring and gating system (SA Instruments, Stony Brook, NY). Mice were maintained at a physiological and stable temperature during the whole protocol by setting the gradient cooling system temperature to 40 C, as suggested by the MR manufacturer.
5
Anesthetic Protocol for Murine Studies
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Animal studies were conducted in agreement with the European Council Directive 2010/63/EU, the French guidelines for animal care from the French Department of Agriculture (Animal Rights Division), and approved by our institutional committee on Ethics in animal research. After isoflurane induction in an anesthetic chamber at 3%, healthy female C57Bl/6J mice (10-20 weeks, 23±2g) were maintained under anaesthesia by spontaneous respiration of a mixture of air and isoflurane (1.5%, constant flow 300mL/min, Univentor 400 anesthesia unit, Zejtun, Malta), through a dedicated nose cone. Respiration and temperature were controlled throughout the experiment with a MR compatible monitoring and gating system (SA Instruments, Stony Brook, NY). A heating blanket connected to a water bath system was placed on the mice's back to maintain physiological temperature throughout the whole experiments (T=37.0 0.5°C).
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