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3 protocols using amphothericin b solution

1

Retinoic Acid Nanoparticle Formulation

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Yellow zein, sodium deoxycholate monohydrate (SD), all-trans-retinoic acid (ATRA), red oil, rhodamine B, 3-[4,5-dimethylthiazol-2-yl]-3,5-diphenyltetrazolium bromide salt (used for MTT-tests), phosphate buffered saline (PBS) tablets, dimethyl sulfoxide, and amphothericin B solution (250 μg/mL), were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Tween 80 (T80) was provided by Acef S.p.a. (Piacenza, Italy). Poloxamer 188 (PLX188) was purchased from BASF (Ludwigshafen, Germany). Ethanol was obtained from Carlo Erba SpA (Rodano [MI], Italy), while cellulose membrane Spectra/Por MWCO 50 kDA was obtained from Spectrum Laboratories Inc. (Eindhoven, the Netherlands).
For the in vitro studies, Dulbecco’s Modified Eagle’s Medium (DMEM) and Roswell Park Memorial Institute1640 enriched with Glutamax I, tripsin/EDTA, penicillin/streptomycin solution and fetal bovine serum (FBS) were obtained from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). A549 and K562 cells were purchased from the IRCCS Azienda Ospedaliera Universitaria San Martino – IST Istituto Nazionale per la Ricerca sul Cancro.
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2

Cytotoxicity Evaluation of Novel Anticancer Agents

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Zein, sodium deoxycholate monohydrate (SD), paclitaxel (PTX), 3-[4,5-dimethylthiazol-2-yl]-3,5-diphenyltetrazolium bromide salt (used for MTT-tests), phosphate buffered saline (PBS) tablets, dimethyl sulfoxide, and amphothericin B solution (250 μg/mL) were all purchased from Sigma Aldrich (Milan, Italy). The bromo- and extra-terminal domain (BET) inhibitor (S)-tertbutyl2-(4-(4-chlorophenyl)-2,3,9-trimethyl6H-thieno(3,2-f)(1,2,4)triazolo(4,3-a)(1,4)diazepin-6-yl)acetate (JQ1), DMEM (Dulbecco’s Modified Eagle’s Medium), RPMI, tripsin/EDTA, penicillin/streptomycin solution, and fetal bovine serum (FBS) were purchased from Thermo Fischer Scientific Inc. (Waltham, MA, USA). Human breast cancer cells (MDA-MB-157 and MDA-MB-231) were purchased from the American Type Culture Collection (Manassas, VI, USA). All other materials and solvents used in this investigation were of analytical grade (Carlo Erba, Milan, Italy).
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3

Zein-based Carrier Evaluation for Cancer

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Yellow zein, sodium deoxycholate monohydrate (SD), 3-[4,5-dimethylthiazol-2-yl]-3,5-diphenyltetrazolium bromide salt (used for MTT-tests), phosphate buffered saline (PBS) tablets, dimethyl sulfoxide, and amphothericin B solution (250 μg/ml) were all purchased from Sigma Aldrich (Milan, Italy). Paclitaxel (PTX) was kindly provided by Indena S.p.a. (Milan, Italy). [3H]cholesteryl hexadecyl ether ([3H]CHE, 40 Ci/mmoL) was obtained from Perkin Elmer-Italia (Monza, Italy).
For the in vitro studies, DMEM (Dulbecco's Modified Eagle's Medium) and RPMI1640 enriched with Glutamax I, tripsin/EDTA, penicillin/streptomycin solution and fetal bovine serum (FBS) were obtained from GIBCO (Life Technology, Milan, Italy). Human breast cancer cells (MCF-7) and chronic myelogenous leukemia (K562) were provided by the IRCCS Azienda Ospedaliera Universitaria San Martino – IST Istituto Nazionale per la Ricerca sul Cancro. All other materials and solvents used in this investigation were of analytical grade (Carlo Erba, Milan, Italy).
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