Leukocytes (~1×106 cells/tube) were stained with DAPI (cell viability), anti-CD45-Pacific Blue, anti-F4/80-FITC, anti-CD11b-PercP Cy5.5, anti-Ly6G-APC, anti-CXCR2-PE (all R&D Systems), anti-CD62L-FITC, anti-GRK2-FITC, anti-Cytokeratin (C-11)-FITC (Abcam), anti-IL-33-PE (R&D Systems) and isotype controls (BD Biosciences), following the manufacturer’s protocols. IL-33 production by CD45− Cytokeratin+ epT cells was determined after stimulation with PMA (50 ng/ml) and ionomycin (1 μg/ml) in the presence of GolgiStop™ and permeabilization of the cells (Cytofix/Cytoperm, BD Biosciences). Cells were acquired using a Beckman Coulter CyAn™ ADP (Beckman Coulter, USA). Gating strategy (Supplementary Figure S5 online ) and analysis were performed using the FlowJo® software (treeStar Software, USA).
Anti cd62l fitc
Manufactured by Abcam
Anti-CD62L-FITC is a fluorescently-labeled antibody that binds to the CD62L (L-selectin) protein. CD62L is a cell adhesion molecule expressed on the surface of various immune cells, including lymphocytes, monocytes, and neutrophils. This antibody can be used in flow cytometry and other immunoassays to detect and quantify CD62L-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Isotype control, by BD (2 mentions)
Cytofix cytoperm, by BD (2 mentions)
Cyan adp, by Beckman Coulter (2 mentions)
Anti cd45 pacific blue, by R&D Systems (2 mentions)
Anti f4 80 fitc, by R&D Systems (2 mentions)
Anti cd11b percp cy5, by R&D Systems (2 mentions)
Anti ly6g apc, by R&D Systems (2 mentions)
Anti cxcr2 pe, by R&D Systems (2 mentions)
Anti grk2 fitc, by Abcam (2 mentions)
Anti cytokeratin c 11 fitc, by Abcam (2 mentions)
Anti il 33 pe, by R&D Systems (2 mentions)
2 protocols using anti cd62l fitc
1
IL-33 Production in Epidermal T Cells
2
IL-33 Production in Epidermal T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Leukocytes (~1×106 cells/tube) were stained with DAPI (cell viability), anti-CD45-Pacific Blue, anti-F4/80-FITC, anti-CD11b-PercP Cy5.5, anti-Ly6G-APC, anti-CXCR2-PE (all R&D Systems), anti-CD62L-FITC, anti-GRK2-FITC, anti-Cytokeratin (C-11)-FITC (Abcam), anti-IL-33-PE (R&D Systems) and isotype controls (BD Biosciences), following the manufacturer’s protocols. IL-33 production by CD45− Cytokeratin+ epT cells was determined after stimulation with PMA (50 ng/ml) and ionomycin (1 μg/ml) in the presence of GolgiStop™ and permeabilization of the cells (Cytofix/Cytoperm, BD Biosciences). Cells were acquired using a Beckman Coulter CyAn™ ADP (Beckman Coulter, USA). Gating strategy (Supplementary Figure S5 online ) and analysis were performed using the FlowJo® software (treeStar Software, USA).
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