Human jurkat t cells clone e6 1

To simulate space conditions, the rotary cell culture system (RCCS), developed by the National Aeronautics and Space Administration (Washington, DC, USA) and manufactured by Synthecon (Houston, TX, USA), was used. Human Jurkat T cells (Clone E6-1) (ATCC, Manassas, VA, USA) were grown in RPMI 1640 medium supplemented with 2 mM glutamine, 2.5 mM sodium pyruvate, 100 U/mL penicillin, 100 μg/mL streptomycin, and 10% heat-inactivated foetal bovine serum. Cells were placed in completely filled 50 mL vessels, to avoid the presence of air bubbles that could lead to shear force damage of cells on the RCCS. Vessels were rotated at a speed of 7.2 rpm (simulated microgravity and referred to as sim-μg), as reported [30 (link), 37 (link)], or cultured at ground gravity (1 g), as controls. Incubation of 1 g and sim-μg cells with different compounds was performed at 37°C in an atmosphere of 5% CO₂, at the indicated concentrations and for the indicated periods of time.

GL261 cells were obtained from NCI Division of Cancer Treatment and Diagnosis Tumor Repository. Human Jurkat T cells (clone E6–1) were obtained from ATCC. Cells were maintained in liquid nitrogen in Bambanker cryopreservation media until further use. Cells were tested for Mycoplasma using Lonza MycoAlert Mycoplasma Detection Assay Kit prior to any use. Cells were used within 3–5 passages.