Penicillin streptomycine glutamine p s g

α-Galactosylceramide (αGC, KRN7000) was purchased from Avanti Polar Lipids. Recombinant human IL-2, IL-3, IL-4, IL-7, IL-15, Flt3-Ligand, Stem Cell Factor (SCF), Thrombopoietin (TPO), and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) were purchased from Peprotech. Ganciclovir (GCV) was purchased from Sigma.
X-VIVO 15 Serum-free Hematopoietic Cell Medium was purchased from Lonza. RPMI 1640 and DMEM cell culture medium were purchased from Corning Cellgro. Fetal bovine serum (FBS) was purchased from Sigma. Medium supplements, including Penicillin-Streptomycine-Glutamine (P/S/G), MEM non-essential amino acids (NEAA), HEPES Buffer Solution, and Sodium Pyruvate, were purchased from GIBCO. Beta-Mercaptoethanol (β-ME) was purchased from Sigma. Normocin was purchased from InvivoGen. Complete lymphocyte culture medium (denoted as C10 medium) was made of RPMI 1640 supplemented with FBS (10% vol/vol), P/S/G (1% vol/vol), MEM NEAA (1% vol/vol), HEPES (10 mM), Sodium Pyruvate (1 mM), β-ME (50 mM), and Normocin (100 mg/ml). Medium for culturing human MM.1S tumor cell line (denoted as R10 medium) was made of RPMI 1640 supplemented with FBS (10% vol/vol) and P/S/G (1% vol/vol). Adherent cell culture medium (denoted as D10 medium) was made of DMEM supplemented with FBS (10% vol/vol) and P/S/G (1% vol/vol).

α-Galactosylceramide (αGC, KRN7000) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Recombinant human IL-2, IL-7, and IL-15 were purchased from PeproTech (Hamburg, Germany).
RPMI1640 and DMEM cell culture media were purchased from Corning Cellgro (Manassas, VA, USA). Fetal bovine serum (FBS) and beta-mercaptoethanol (β-ME) were purchased from Sigma (St. Louis, MO, USA). Medium supplements, including penicillin–streptomycine–glutamine (P/S/G), MEM non-essential amino acids (NEAA), HEPES Buffer Solution, and sodium pyruvate, were purchased from GIBCO (Waltham, MA, USA). Normocin was purchased from InvivoGen (San Diego, CA, USA). The complete lymphocyte culture medium (denoted as the C10 medium) comprised RPMI 1640 supplemented with FBS (10% v/v), P/S/G (1% v/v), MEM NEAA (1% v/v), HEPES (10 mM), sodium pyruvate (1 mM), β-ME (50 μM), and Normocin (100 μg/mL). The adherent cell culture medium (denoted as the D10 medium) comprised DMEM supplemented with FBS (10% v/v), P/S/G (1% v/v), and Normocin (100 μg/mL).