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Pe conjugated anti mouse pd 1

Manufactured by Thermo Fisher Scientific
Sourced in United States

The PE-conjugated anti-mouse PD-1 is a fluorescence-labeled antibody that binds to the programmed cell death-1 (PD-1) receptor on mouse cells. It can be used to detect and analyze PD-1 expression in mouse samples through flow cytometry or other immunoassay techniques.

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2 protocols using pe conjugated anti mouse pd 1

1

Multicolor Flow Cytometry Analysis

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Multicolor flow cytometry was performed using the following commercially available antibodies: phycoerythrin (PE)-conjugated anti-human PD-1, allophycocyanin (APC)-cyanine 7 (Cy7)-conjugated anti-mouse F4/80 and CD4 (BioLegend, San Diego, CA, USA), PE-conjugated anti-mouse PD-1, PerCP-Cy5.5-conjugated anti-mouse CD8a (eBioscience, San Diego, CA, USA), V500-conjugated anti-human CD3, V450-conjugated anti-human CD8, APC-H7-conjugated anti-human CD4, APC-conjugated anti-human CD69, V450-conjugated anti-mouse CD3 and CD11b, APC-conjugated anti-mouse CD69, V500-conjugated anti-mouse CD45, APC-conjugated anti PD-L1, and PE-Cy7-conjugated anti-mouse Ly6G (BD Biosciences, San Jose, CA, USA). Dead cells were excluded using the aqua fluorescent LIVE/DEAD dye (Invitrogen). Multicolor flow cytometry was performed using the LSR Fortessa, Canto II instrument (BD Biosciences). Data were analyzed using FlowJo software (TreeStar, Ashland, OR, USA).
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2

Multi-parametric flow cytometry analysis

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Single cell suspensions were labeled with Fc block (purified anti-mouse CD16) (BD Pharmingen, San Diego, CA) and antibodies for 20 min and washed twice with a staining buffer consisting of 5 % FBS (Gemini Bio-products, Sacramento, CA) in DPBS (Mediatech, Herndon, VA). Samples were analyzed using a custom-configured Fortessa using FACSDiva® software (Becton–Dickinson, San Jose, CA). The BD Cytofix/Cytoperm™ kit (Becton–Dickinson) was used for granzyme staining per manufacturer’s instructions. Data were analyzed using FlowJo software (TreeStar, Ashland, OR). Antibodies included: PE-Cy5-conjugated anti-mouse CD62L, APC-Cy7-conjugated anti-mouse CD25 (clone PC61), PE-Cy5- conjugated anti-mouse CD44, PE and PE-Cy7-conjugated anti-mouse NKG2D, PE-conjugated anti-mouse TCR Vα2, PE-conjugated anti-mouse PD-1 (eBioscience, San Diego, CA); FITC-conjugated anti-mouse TCR Vβ5.1/5.2, FITC-conjugated anti-mouse CD4, and APC-Cy7-conjugated CD25 (clone PC61) (BD Pharmingen, San Diego, CA); Alexa Fluor 700-conjugated anti-mouse CD8, Pacific Blue-conjugated anti-mouse CD44 and anti-mouse CD45.2 (BioLegend, San Diego, CA).
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