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Pe conjugated anti mouse pd 1

Manufactured by Thermo Fisher Scientific
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The PE-conjugated anti-mouse PD-1 is a fluorescence-labeled antibody that binds to the programmed cell death-1 (PD-1) receptor on mouse cells. It can be used to detect and analyze PD-1 expression in mouse samples through flow cytometry or other immunoassay techniques.

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Lab products found in correlation

V450 conjugated anti human cd8, by BD (1 mentions) Apc h7 conjugated anti human cd4, by BD (1 mentions) Apc conjugated anti human cd69, by BD (1 mentions) Apc conjugated anti pd l1, by BD (1 mentions) Pe cy7 conjugated anti mouse ly6g, by BD (1 mentions) Fitc conjugated anti mouse cd4, by BD (1 mentions) Cytofix cytoperm kit, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions)

2 protocols using pe conjugated anti mouse pd 1

1

Multicolor Flow Cytometry Analysis

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Multicolor flow cytometry was performed using the following commercially available antibodies: phycoerythrin (PE)-conjugated anti-human PD-1, allophycocyanin (APC)-cyanine 7 (Cy7)-conjugated anti-mouse F4/80 and CD4 (BioLegend, San Diego, CA, USA), PE-conjugated anti-mouse PD-1, PerCP-Cy5.5-conjugated anti-mouse CD8a (eBioscience, San Diego, CA, USA), V500-conjugated anti-human CD3, V450-conjugated anti-human CD8, APC-H7-conjugated anti-human CD4, APC-conjugated anti-human CD69, V450-conjugated anti-mouse CD3 and CD11b, APC-conjugated anti-mouse CD69, V500-conjugated anti-mouse CD45, APC-conjugated anti PD-L1, and PE-Cy7-conjugated anti-mouse Ly6G (BD Biosciences, San Jose, CA, USA). Dead cells were excluded using the aqua fluorescent LIVE/DEAD dye (Invitrogen). Multicolor flow cytometry was performed using the LSR Fortessa, Canto II instrument (BD Biosciences). Data were analyzed using FlowJo software (TreeStar, Ashland, OR, USA).
Park D.J., Sung P.S., Lee G.W., Cho S.W., Kim S.M., Kang B.Y., Hur W.H., Yang H., Lee S.K., Lee S.H., Jung E.S., Seo C.H., Ahn J., Choi H.J., You Y.K., Jang J.W., Bae S.H., Choi J.Y, & Yoon S.K. (2021). Preferential Expression of Programmed Death Ligand 1 Protein in Tumor-Associated Macrophages and Its Potential Role in Immunotherapy for Hepatocellular Carcinoma. International Journal of Molecular Sciences, 22(9), 4710.
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2

Multi-parametric flow cytometry analysis

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Single cell suspensions were labeled with Fc block (purified anti-mouse CD16) (BD Pharmingen, San Diego, CA) and antibodies for 20 min and washed twice with a staining buffer consisting of 5 % FBS (Gemini Bio-products, Sacramento, CA) in DPBS (Mediatech, Herndon, VA). Samples were analyzed using a custom-configured Fortessa using FACSDiva® software (Becton–Dickinson, San Jose, CA). The BD Cytofix/Cytoperm™ kit (Becton–Dickinson) was used for granzyme staining per manufacturer’s instructions. Data were analyzed using FlowJo software (TreeStar, Ashland, OR). Antibodies included: PE-Cy5-conjugated anti-mouse CD62L, APC-Cy7-conjugated anti-mouse CD25 (clone PC61), PE-Cy5- conjugated anti-mouse CD44, PE and PE-Cy7-conjugated anti-mouse NKG2D, PE-conjugated anti-mouse TCR Vα2, PE-conjugated anti-mouse PD-1 (eBioscience, San Diego, CA); FITC-conjugated anti-mouse TCR Vβ5.1/5.2, FITC-conjugated anti-mouse CD4, and APC-Cy7-conjugated CD25 (clone PC61) (BD Pharmingen, San Diego, CA); Alexa Fluor 700-conjugated anti-mouse CD8, Pacific Blue-conjugated anti-mouse CD44 and anti-mouse CD45.2 (BioLegend, San Diego, CA).
Sckisel G.D., Mirsoian A., Bouchlaka M.N., Tietze J.K., Chen M., Blazar B.R, & Murphy W.J. (2015). Late administration of murine CTLA-4 blockade prolongs CD8-mediated anti-tumor effects following stimulatory cancer immunotherapy. Cancer immunology, immunotherapy : CII, 64(12), 1541-1552.
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