All reagents were of analytical grade, and water was obtained with a Milli-Q system from Millipore (Bedford, MA, USA). Acetonitrile (ACN) was from Scharlau (Barcelona, Spain), di-sodium tetraborate decahydrate (borate buffer) from Merck (Darmstadt, Germany), and standard amino acids (AA-18) from Fluka (Buchs, Switzerland). Glutaraldehyde, sodium borohydrate (NaHB4), sodium tetraborate, β-mercaptoethanol, o-phthaldialdehyde (OPA), L-glutathione (GSH), and bovine serum albumin (BSA) were acquired from Sigma-Aldrich (St Louis, MO, USA). Hydrochloric acid (HCl) and calcium chloride (CaCl2) were from Panreac (Darmstadt, Germany). Alcalase was kindly donated by Novozymes Spain S.A. (Madrid, Spain).
Acetonitrile acn
Manufactured by Scharlab
Sourced in Spain
Acetonitrile (ACN) is a colorless, volatile, and flammable organic solvent. It is commonly used in various laboratory applications, serving as a mobile phase component in high-performance liquid chromatography (HPLC) and as a solvent for organic synthesis reactions.
Automatically generated - may contain errors
Lab products found in correlation
Sodium tetraborate, by Merck Group (1 mentions)
Calcium chloride cacl2, by ITW Reagents (1 mentions)
Hydrochloric acid hcl, by ITW Reagents (1 mentions)
L glutathione gsh, by Merck Group (1 mentions)
Di sodium tetraborate decahydrate, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
O phthaldialdehyde opa, by Merck Group (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Alcalase, by Novozymes (1 mentions)
Zinc acetate znac, by Merck Group (1 mentions)
8453 uv vis spectroscopy system, by Agilent Technologies (1 mentions)
Acetic acid, by Avantor (1 mentions)
Phosphoric acid, by Merck Group (1 mentions)
Perchloric acid pca, by Merck Group (1 mentions)
Disodium hydrogen phosphate dihydrate, by Avantor (1 mentions)
1100 hplc system, by Agilent Technologies (1 mentions)
Potassium dihydrogen phosphate, by AppliChem (1 mentions)
2 protocols using acetonitrile acn
1
Amino Acid Analysis Protocol
2
HPLC Analysis of Tryptophan Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The chromatographic analyses were performed using an Agilent 1100 HPLC system (Santa Clara, CA, USA) with Agilent G1314A UVD and G1321A FLD. The spectral analyses of the UV-detected compounds were made with an Agilent 8453 UV-Vis Spectroscopy System (Santa Clara, CA, USA). The reference compounds TRP, 5-HT, KYN, KYNA, 3NLT; perchloric acid (PCA), zinc acetate (ZnAc) and phosphoric acid were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Acetonitrile (ACN) was obtained from Scharlau (Barcelona, Spain) and acetic acid from VWR International (Radnor, PA, USA). The di-sodium-hydrogen phosphate dihydrate was obtained from VWR International (Radnor, PA, USA) and potassium dihydrogen phosphate from Applichem Panreac (Darmstadt, Germany). The IS used for the FLD (HCA) was synthesized at the Department of Pharmaceutical Chemistry, University of Szeged, involving the ring closure of anthranilamide with diethyl oxalate, followed by the hydrolysis of the ester function [33] (link).
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