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6 protocols using skf 525 a

1

Insecticide Compound Preparation Protocol

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Dicyclanil, imidacloprid, spinosad, ivermectin, piperonyl butoxide (PBO), tranylcypromine, MGK264, aminobenzotriazole, ketoconazole, diethyl maleate and SKF525 A (proadifen) were purchased from Sigma‐Aldrich (St Louis, MO 63103, USA). Cyromazine was purchased from Chem Service (West Chester, PA 19381, USA). Trichlorophenylpropynyl ether (TCPPE) was supplied by Elanco Animal Health (Greenfield, IN 46140, USA). All chemicals were prepared at 10 mg mL−1 in ethanol and stored at −20 °C. Various dilutions subsequently were prepared by serial dilution in ethanol.
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2

Pharmacological Reagents for Research

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D-Methamphetamine (D-meth), L-methamphetamine (L-meth), L-cycloserine, myriocin, thalidomide, cimetidine, quinidine, SKF-525A and clotrimazole were purchased from Sigma Aldrich (St. Louis, Missouri). Fumonisin B1 (FB1), C6 and C8 ceramide, and HET-0016 were from Cayman Chemicals (Ann Arbor, Michigan). 5′-aminosalicylic acid and JSH-23 were from Santa Cruz Biotechnology (Santa Crux, CA).
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3

Quantitative Analysis of Morphine and 6-MAM

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All the standards molecules (morphine 1 mg/mL in methanol and 6-MAM 1 mg/mL in acetonitrile) involved in this study were purchased from Sigma-Aldrich and stored at -20 °C. The Internal Standard (IS) SKF 525-A (Proadifen hydrochloride, analytical standard, > 95%, 100 mg) was purchased from Sigma-Aldrich as well.
Working solutions of each molecule and IS were prepared with methanol or acetonitrile at 100 µg/mL and 1 µg/mL, starting from standard solutions, and stored at -20 °C until use.
Solvents used in the extraction processes were purchased from Sigma-Aldrich (methanol) and from PanReac AppliChem ITW Reagents (phosphate buffer solution pH 4, phosphate buffer solution pH 9 and water for UV, HPLC, ACS).
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4

Quantification of Bioactive Molecules

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All the standards molecules involved in this study and the Internal Standard (IS) SKF 525-A (Proadifen hydrochloride, analytical standard, > 95%, 100 mg) as well, were purchased from Sigma-Aldrich and stored at − 20 °C. Working solutions of each molecule and IS were prepared, starting from standard solutions, and stored at − 20 °C until use. Solvents used in the extraction processes were purchased by Sigma-Aldrich (methanol) and by PanReac AppliChem ITW Reagents (buffer solution pH 9 and water for UV, HPLC, ACS).
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5

Quantitative Analysis of Cocaine and Lidocaine

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Analytical standards involved in this study as Cocaine Multi-component Mixture solution (250 µg/mL in acetonitrile, certified reference material Cerilliant®), and Lidocaine solution (1 mg/mL in methanol, certified reference material Cerilliant®), were purchased from Sigma-Aldrich and stored at -20 °C. Internal Standard (IS) SKF 525-A (Proadifen hydrochloride, analytical standard, > 95%, 100 mg) was purchased from Sigma-Aldrich as well. Working solutions of the analytical standards and IS were prepared in acetonitrile or methanol and stored at -20 °C until use. Solvents used in the extraction processes were purchased by PanReac AppliChem ITW Reagents (methanol, phosphate buffer solution pH 9 and phosphate buffer pH 6, 2-propanol, n-hexane, n-heptane, and water).
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6

MDMA Quantification Protocol

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MDMA as hydrochloride (Reference Standards, Lot No: 171242-201163) was obtained from Chinese Drugs & Biological Product Standardization Inst and the Internal Standard (I.S.); SKF525A (propyl-adiphenine, Lot No: 028K1661) and trifluoroacetic acid (TFA, Lot No: 0001403330) were purchased from Sigma Co.; sodium chloride, sodium hydroxide, ethanol, methanol and I.S. Isopropanol (all analytical grade) were purchased from Merck (Darmstadt, Germany). Water was purified by a Milli-Q water system (Millipore, Billerica, MA).
To prepare stock solutions, MDMA was dissolved in methanol at 2.0 mg/mL and SKF525A at 1.0 mg/mL; and Isopropanol was prepared at 1.25 mol/L in deionized water. All the solutions were re-prepared every 2 weeks for stable quality controls.
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