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Elisas

Manufactured by Cayman Chemical
Sourced in France

ELISAs (Enzyme-Linked Immunosorbent Assays) are a type of analytical biochemistry technique used to detect and quantify specific substances, typically proteins, hormones, or antibodies, in a liquid sample. ELISAs rely on the principle of antigen-antibody interactions and the use of enzymes to produce a measurable signal.

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3 protocols using elisas

1

Quantifying Ovarian Steroid Release

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The progesterone and estradiol concentrations in culture medium from ovarian explants were measured after 48 h of treatment using ELISAs (Cayman Chemicals, Montluçons, France) based on a previous study (Canepa et al., 2008 ). The intra- and inter-assay coefficients of variation were <10% and <8%, respectively. The results are expressed as the concentration of progesterone and estradiol (ng/mL) per 100 μg of protein. The data were obtained from 5 independent cultures.
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2

Quantifying Inflammatory Mediators

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TNF-α, IL-6, CXCL1, MIF, MPO, and AREG were detected using Duoset enzyme-linked immunosorbent assay (ELISA) kits from R&D systems (Minneapolis, MN). Resolvin D1 (RvD1) and Resolvin D2 (RvD2) were detected using ELISAs from Cayman Chemical (Ann Arbor, MI).
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3

Quantifying Inflammatory Mediators

Check if the same lab product or an alternative is used in the 5 most similar protocols
TNF-α, IL-6, CXCL1, MIF, MPO, and AREG were detected using Duoset enzyme-linked immunosorbent assay (ELISA) kits from R&D systems (Minneapolis, MN). Resolvin D1 (RvD1) and Resolvin D2 (RvD2) were detected using ELISAs from Cayman Chemical (Ann Arbor, MI).
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