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Ab84571

Manufactured by Abcam
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Sourced in United Kingdom

Ab84571 is a laboratory equipment product. It is designed for use in scientific research applications. The core function of this product is to facilitate specific tasks or measurements within a laboratory setting. No further details on the intended use or interpretation of this product can be provided in an unbiased and factual manner.

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Lab products found in correlation

Ab23905, by Abcam (1 mentions)

2 protocols using ab84571

1

Immunohistochemical Scoring of GPSM2 and DYNC1I1

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IHC staining was performed as described previously (19 (link)). Anti-human GPSM2 rabbit antibody was used at a dilution of 1:100 (ab84571; Abcam, Cambridge, UK); Anti-human DYNC1I1 mouse antibody was used at a dilution of 1:100 (ab23905; Abcam, Cambridge, UK); phosphate-buffered saline was used as a negative control. Each section was evaluated and scored independently by two pathologists. A semi-quantitative scoring system was used in this assay. Intensity was scored as “0” (negative), “1” (weak), “2” (moderate), and “3” (strong), and the percentages of tumor cells within each category were calculated. The percentage score was multiplied by the staining intensity score to generate the IHC score. The histological score range was from zero (minimum) to 300 (maximum). Positive expression was defined as detectable immunoreactions with an IHC score > 10.
Deng M., Zhang Z., Liu B., Hou K., Che X., Qu X., Liu Y., Hu X., Zhang Y, & Lv Q. (2020). Localization of GPSM2 in the Nucleus of Invasive Breast Cancer Cells Indicates a Poor Prognosis. Frontiers in Oncology, 10, 227.
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2

Immunohistochemical Evaluation of GPSM2

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Immunohistochemical staining was carried out as described previously.17 Staining was carried out with an anti‐human GPSM2 rabbit Ab that was used at a dilution of 1:100 (ab84571; Abcam); PBS was used as a negative control. Each section was evaluated and scored independently by 2 pathologists. A semiquantitative scoring system was used in this assay. Intensity was scored as: 0, negative; 1, weak; 2, moderate; and 3, strong. We also calculated the proportion of tumor cells within each category. The proportional score was then multiplied by the staining intensity score to generate a final immunohistochemistry (IHC) score. The IHC scores ranged from 0 (minimum) to 300 (maximum). Positive expression of GPSM2 was defined as detectable immunoreactions with an IHC score greater than 10.
Deng M., Liu B., Zhang Z., Chen Y., Wang Y., Wang X., Lv Q., Yang X., Hou K., Che X., Qu X., Liu Y., Zhang Y, & Hu X. (2020). Knockdown of G‐protein‐signaling modulator 2 promotes metastasis of non‐small‐cell lung cancer by inducing the expression of Snail. Cancer Science, 111(9), 3210-3221.
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