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Anti il 17a apc vio770

Manufactured by Miltenyi Biotec
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Anti-IL-17A-APC-Vio770 is a fluorescent-labeled antibody specific for interleukin-17A (IL-17A). It can be used for the detection and quantification of IL-17A-expressing cells in flow cytometry applications.

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Lab products found in correlation

Foxp3 transcription factor staining buffer kit, by Thermo Fisher Scientific (2 mentions) Life dead fixable aqua dead cell kit, by Thermo Fisher Scientific (2 mentions) Cd3 percp vio700 antibody, by Miltenyi Biotec (2 mentions) Anti tnf α eflour 450 clone mab11, by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Merck Group (2 mentions) Penicillin, by Merck Group (2 mentions) Streptomycin, by Merck Group (2 mentions) Ionomycin, by Merck Group (2 mentions) Phorbol myristate acetate (pma), by Merck Group (2 mentions) Facsdiva software, by BD (2 mentions) Facscanto 2 flow cytometer, by BD (2 mentions) Golgistop, by BD (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Biocoll, by Merck Group (2 mentions)

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IL-17A (3 citations)

2 protocols using anti il 17a apc vio770

1

Isolation and Cytokine Analysis of CD4+ T Cells

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Peripheral venous blood was obtained from study participants. Peripheral blood mononuclear cells (PBMCs) were immediately isolated by density centrifugation using Biocoll (Merck, Darmstadt, Germany). 106 CD4+ enriched cell fractions isolated by CD4+ T Cell Kit (Miltenyi) were plated onto U-bottom plates and were restimulated for 4 hours at 37°C, 5% CO2 in a humidified incubator. Restimulation was conducted in a final volume of 200 μl RPMI 1640 (Sigma) supplemented with 10% FBS (Merck), 100 U/ml penicillin (Sigma), 100 mg/ml streptomycin (Sigma), 50 ng/ml PMA (Sigma), 250 ng/ml ionomycin (Sigma) and 1.3 μl/ml Golgistop (BD). After restimulation cells were stained with Life/Dead Fixable Aqua Dead cell kit (Thermo Fisher) and monoclonal CD3-PerCP-Vio700 antibody (clone BW264/56, Miltenyi). Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer kit (eBioscience) and labeled using anti-IL-17A-APC-Vio770 (clone CZ8-2361, Miltenyi) and anti-TNF-α-eFlour 450 (clone Mab11, eBioscience) monoclonal antibodies. Cells were analyzed using a FACSCanto II flow cytometer and FACSDiva software (BD). Data analysis was performed with FlowJo (LLC).
Wilck N., Matus M.G., Kearney S.M., Olesen S.W., Forslund K., Bartolomaeus H., Haase S., Mähler A., Balogh A., Markó L., Vvedenskaya O., Kleiner F.H., Tsvetkov D., Klug L., Costea P.I., Sunagawa S., Maier L., Rakova N., Schatz V., Neubert P., Frätzer C., Krannich A., Gollasch M., Grohme D.A., Côrte-Real B.F., Gerlach R.G., Basic M., Typas A., Wu C., Titze J.M., Jantsch J., Boschmann M., Dechend R., Kleinewietfeld M., Kempa S., Bork P., Linker R.A., Alm E.J, & Müller D.N. (2017). Salt-responsive gut commensal modulates TH17 axis and disease. Nature, 551(7682), 585-589.
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2

Isolation and Cytokine Analysis of CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral venous blood was obtained from study participants. Peripheral blood mononuclear cells (PBMCs) were immediately isolated by density centrifugation using Biocoll (Merck, Darmstadt, Germany). 106 CD4+ enriched cell fractions isolated by CD4+ T Cell Kit (Miltenyi) were plated onto U-bottom plates and were restimulated for 4 hours at 37°C, 5% CO2 in a humidified incubator. Restimulation was conducted in a final volume of 200 μl RPMI 1640 (Sigma) supplemented with 10% FBS (Merck), 100 U/ml penicillin (Sigma), 100 mg/ml streptomycin (Sigma), 50 ng/ml PMA (Sigma), 250 ng/ml ionomycin (Sigma) and 1.3 μl/ml Golgistop (BD). After restimulation cells were stained with Life/Dead Fixable Aqua Dead cell kit (Thermo Fisher) and monoclonal CD3-PerCP-Vio700 antibody (clone BW264/56, Miltenyi). Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer kit (eBioscience) and labeled using anti-IL-17A-APC-Vio770 (clone CZ8-2361, Miltenyi) and anti-TNF-α-eFlour 450 (clone Mab11, eBioscience) monoclonal antibodies. Cells were analyzed using a FACSCanto II flow cytometer and FACSDiva software (BD). Data analysis was performed with FlowJo (LLC).
Wilck N., Matus M.G., Kearney S.M., Olesen S.W., Forslund K., Bartolomaeus H., Haase S., Mähler A., Balogh A., Markó L., Vvedenskaya O., Kleiner F.H., Tsvetkov D., Klug L., Costea P.I., Sunagawa S., Maier L., Rakova N., Schatz V., Neubert P., Frätzer C., Krannich A., Gollasch M., Grohme D.A., Côrte-Real B.F., Gerlach R.G., Basic M., Typas A., Wu C., Titze J.M., Jantsch J., Boschmann M., Dechend R., Kleinewietfeld M., Kempa S., Bork P., Linker R.A., Alm E.J, & Müller D.N. (2017). Salt-responsive gut commensal modulates TH17 axis and disease. Nature, 551(7682), 585-589.
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