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Recombinant human il 2 rhil 2

Manufactured by Novartis
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Recombinant human IL-2 (rhIL-2) is a type of lab equipment used in research and development. It is a cytokine protein that is produced through recombinant DNA technology. The core function of rhIL-2 is to stimulate the growth and differentiation of T cells.

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Lab products found in correlation

Ficoll hypaque, by Merck Group (2 mentions) Anti cd3 monoclonal antibody, by Miltenyi Biotec (1 mentions) Anti cd3 mab, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

Recombinant human IL-2 Recombinant IL-2 Human IL-2 RhIL-2

2 protocols using recombinant human il 2 rhil 2

1

Lymphocyte Isolation and T-cell Activation

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Peripheral blood lymphocytes (PBLs) were isolated from apheresis products (Key Biologics, Memphis, TN) using Ficoll-Hypaque (Sigma-Aldrich, St Louis, MO), as previously described.24 T cells were activated by stimulating PBLs with 50 ng/mL anti-CD3 monoclonal antibody (Miltenyi Biotec, Bergisch Gladbach, Germany), 300 IU/mL recombinant human IL-2 (rhIL-2; Novartis Pharmaceuticals, East Hanover, NJ), and 100 ng/mL rhIL-15 (Biological Resources Branch, National Cancer Institute, Bethesda, MD) for 3 days before transduction. Alternatively, PBLs were cultured with 20 ng/mL rhIL-7 (Biological Resources Branch, National Cancer Institute) for 7 days before transduction.24
Wang S.Y., Scurti G.M., Dalheim A.V., Quinn S., Stiff P.J, & Nishimura M.I. (2023). Nonactivated and IL-7 cultured CD19-specific CAR T cells are enriched in stem cell phenotypes and functionally superior. Blood Advances, 8(2), 324-335.
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2

Isolation and Activation of T Cells

Cited 1 time
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Peripheral blood lymphocytes (PBLs) from independent normal human donors were isolated from purchased apheresis products (Key Biologics, Memphis, TN) using Ficoll-Hypaque (Sigma-Aldrich, St. Louis, MO) density gradients40 (link),43 (link). T cells were activated by stimulating PBLs from an individual donor with 50 ng/mL anti-CD3 mAb (Miltenyi Biotec, Bergisch Gladbach, Germany), 300 IU/mL recombinant human IL-2 (rhIL-2; Novartis Pharmaceuticals, East Hanover, NJ), and 100 ng/mL recombinant human IL-15 (rhIL-15; Biological Resources Branch, National Cancer Institute, Bethesda, MD) for three days prior to transduction43 (link). Alternatively, PBLs from the same donor were cultured with 20 ng/mL recombinant human IL-7 (rhIL-7; Biological Resources Branch, National Cancer Institute) for seven days prior to transduction.
Wang S.Y., Moore T.V., Dalheim A.V., Scurti G.M, & Nishimura M.I. (2021). Melanoma reactive TCR-modified T cells generated without activation retain a less differentiated phenotype and mediate a superior in vivo response. Scientific Reports, 11, 13327.
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