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Cxcr2 clone 48311

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CXCR2 (clone 48311) is a laboratory reagent used for the detection and quantification of the CXCR2 protein. CXCR2 is a G protein-coupled receptor that plays a role in the inflammatory response. This reagent can be used in various immunoassay techniques to measure CXCR2 levels in biological samples.

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Lab products found in correlation

Fitc conjugated goat anti mouse antibody, by Agilent Technologies (2 mentions) Qifikit, by Agilent Technologies (2 mentions) C5ar clone s5 1, by Bio-Rad (2 mentions) C5l2 clone 1d9 m12, by BioLegend (2 mentions) Cxcr1 clone 42705, by R&D Systems (2 mentions)

Spelling variants of this product

CXCR2 (10 citations) Clone 48311 (2 citations)

2 protocols using cxcr2 clone 48311

1

Quantifying Chemokine Receptor Expression

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Freshly isolated human leukocytes were incubated in a total volume of 50 µL at 5×106 cell mL−1 on ice with 10 µg mL−1 mouse anti-human chemokine receptor monoclonal antibodies for C5aR (clone S5/1, AbD SeroTec), C5L2 (clone 1D9-M12, Bio Legend), CXCR1 (clone 42705, R&D), CXCR2 (clone 48311, R&D), and CCR2 (clone 48607, R&D), followed by FITC-conjugated goat-anti-mouse antibody (1:50, Dako). Antibody binding was quantified by calibration to defined antibody binding capacity units using QIFIKIT (Dako) and corrected for isotype controls.
Spaan A.N., Vrieling M., Wallet P., Badiou C., Reyes-Robles T., Ohneck E.A., Benito Y., de Haas C.J., Day C.J., Jennings M.P., Lina G., Vandenesch F., van Kessel K.P., Torres V.J., van Strijp J.A, & Henry T. (2014). The Staphylococcal Toxins γ-Hemolysin AB and CB Differentially Target Phagocytes by Employing Specific Chemokine Receptors. Nature communications, 5, 5438.
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2

Quantifying Chemokine Receptor Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freshly isolated human leukocytes were incubated in a total volume of 50 µL at 5×106 cell mL−1 on ice with 10 µg mL−1 mouse anti-human chemokine receptor monoclonal antibodies for C5aR (clone S5/1, AbD SeroTec), C5L2 (clone 1D9-M12, Bio Legend), CXCR1 (clone 42705, R&D), CXCR2 (clone 48311, R&D), and CCR2 (clone 48607, R&D), followed by FITC-conjugated goat-anti-mouse antibody (1:50, Dako). Antibody binding was quantified by calibration to defined antibody binding capacity units using QIFIKIT (Dako) and corrected for isotype controls.
Spaan A.N., Vrieling M., Wallet P., Badiou C., Reyes-Robles T., Ohneck E.A., Benito Y., de Haas C.J., Day C.J., Jennings M.P., Lina G., Vandenesch F., van Kessel K.P., Torres V.J., van Strijp J.A, & Henry T. (2014). The Staphylococcal Toxins γ-Hemolysin AB and CB Differentially Target Phagocytes by Employing Specific Chemokine Receptors. Nature communications, 5, 5438.
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