Alkaline phosphatase or horseradish peroxidase hrp conjugated anti mouse rabbit secondary antibodies

Manufactured by Jackson ImmunoResearch

Alkaline phosphatase (AP) or horseradish peroxidase (HRP) conjugated anti‐mouse/rabbit secondary antibodies are laboratory reagents used to detect and quantify target proteins in various immunoassays. These secondary antibodies are specifically designed to bind to the primary antibodies raised against mouse or rabbit antigens, thus amplifying the signal for improved sensitivity and visualization.

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Lab products found in correlation

Triton x 100, by Merck Group (2 mentions) Trypan blue, by Merck Group (2 mentions) Histopaque 1077, by Merck Group (2 mentions) Venetoclax, by Selleck Chemicals (2 mentions) Ibrutinib, by Selleck Chemicals (2 mentions) A 1331852, by Selleck Chemicals (2 mentions) Cleaved parp 1, by Cell Signaling Technology (2 mentions) Cell titer glo luminescent viability assay reagent, by Promega (2 mentions) Bcl xl, by Cell Signaling Technology (2 mentions) Bcl 2, by Cell Signaling Technology (2 mentions) Selinexor, by Selleck Chemicals (2 mentions) Mcl 1, by Santa Cruz Biotechnology (2 mentions) Primary mouse anti human antibodies raised, by Merck Group (2 mentions) Parp1, by Cell Signaling Technology (2 mentions) Sodium 51chromate 51cr, by PerkinElmer (1 mentions)

Spelling variants of this product

HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (16 citations) Horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (9 citations) Secondary horseradish peroxidase (HRP)-conjugated antibodies (8 citations) Anti-rabbit or anti-mouse secondary antibodies (7 citations) HRP-conjugated anti-rabbit or anti-mouse antibodies (6 citations) Peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (5 citations) Anti-rabbit or anti-mouse HRP antibodies (5 citations) Secondary alkaline phosphatase conjugated antibodies (4 citations) Anti-mouse or anti-rabbit horseradish peroxidase (HRP)–conjugated secondary antibodies (2 citations) Anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibodies (2 citations)

2 protocols using alkaline phosphatase or horseradish peroxidase hrp conjugated anti mouse rabbit secondary antibodies

Comprehensive Antibody Source Protocol

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Pevonedistat was provided by Millennium Pharmaceuticals, Inc. (Cambridge, MA), a wholly owned subsidiary of Takeda Pharmaceutical Company Limited. Venetoclax, Selinexor, A‐1331852, and Ibrutinib were obtained by Selleck Chemicals (Houston, TX). All other drugs were provided by the RPCCC Pharmacy.
Primary mouse anti‐human antibodies raised against Bak and Bax were obtained from Sigma Chemicals (St. Louis, MO), Mcl‐1 from Santa Cruz Biotechnology (Santa Barbara, CA). Primary mouse/rabbit anti‐human antibodies raised against Bid, Bim, Noxa, Beclin‐1, p65, Bcl‐2, Bcl‐XL, Puma, PARP‐1, cleaved PARP‐1 and actin were obtained from Cell Signaling (Danvers, MA). Alkaline phosphatase (AP) or horseradish peroxidase (HRP) conjugated anti‐mouse/rabbit secondary antibodies were purchased from Jackson ImmunoResearch (West Grove, PA).
Sodium ⁵¹chromate (⁵¹Cr) (Perkin‐Elmer Life Inc., Boston MA) was used in functional assays assessing antibody‐mediated complement cytotoxicity (CMC) or antibody dependent cellular cytotoxicity (ADCC). Triton X‐100, trypan blue and histopaque‐1077 were obtained from Sigma‐Aldrich Inc. (St Louis, MO). Cell Titer‐Glo Luminescent Viability Assay reagent was purchased from Promega (Madison, WI).

Torka P., Mavis C., Kothari S., Belliotti S., Gu J., Sundaram S., Barth M, & Hernandez‐Ilizaliturri F.J. (2020). Pevonedistat, a NEDD8‐activating enzyme inhibitor, induces apoptosis and augments efficacy of chemotherapy and small molecule inhibitors in pre‐clinical models of diffuse large B‐cell lymphoma. EJHaem, 1(1), 122-132.

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Cellular Cytotoxicity Assays Reagents

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Pevonedistat was provided by Millennium Pharmaceuticals, Inc., Cambridge, MA, a wholly owned subsidiary of Takeda Pharmaceutical Company Limited. Venetoclax, Selinexor, A-1331852, and Ibrutinib were obtained by Selleck Chemicals (Houston, TX). All other drugs were provided by the RPCCC Pharmacy.
Primary mouse anti-human antibodies raised against Bak and Bax were obtained from Sigma Chemicals (St. Louis, MO), Mcl-1 from Santa Cruz Biotechnology (Santa Barbara, CA). Primary mouse/rabbit anti-human antibodies raised against Bid, Bim, Noxa, Beclin-1, p65, Bcl-2, Bcl-XL, Puma, PARP-1, cleaved PARP-1 and actin were obtained from Cell Signaling (Danvers, MA). Alkaline phosphatase (AP) or horseradish peroxidase (HRP) conjugated anti-mouse/rabbit secondary antibodies were purchased from Jackson ImmunoResearch (West Grove, PA).
Sodium ⁵¹chromate (⁵¹Cr) (Perkin-Elmer Life Inc., Boston MA) was used in functional assays assessing antibody-mediated complement cytotoxicity (CMC) or antibody dependent cellular cytotoxicity (ADCC). Triton X-100, trypan blue and histopaque-1077 were obtained from Sigma-Aldrich Inc. (St Louis, MO). Cell Titer-Glo Luminescent Viability Assay reagent was purchased from Promega (Madison, WI).

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