Total RNA was extracted from cultured cells using Trizol reagent (Invitrogen, Carlsbad, CA, USA). RNA quantity and purity were determined by absorbance measurements at 260 nm and 260/280 nm, respectively, with a NanoDrop ND-100 spectrophotometer (NanoDrop Technologies, Rockland, DE, USA). A PrimeScriptTM RT Reagent Kit (Takara, Japan) was used for mRNA reverse transcription. Relative mRNA expression was calculated via the 2−ΔΔCT method after normalization to 18S rRNA. qRT- PCR was carried out on an ABI PRISM 7500 system using SYBR® Premix Ex TaqTM II (Takara, Japan). The following PCR primers were used:
CD36 forward: 5′- TGCAAGTCCTGATGTTTCAGA -3′;
reverse: 5′- TGGCTTGACCAATAGGTTGAC -3′.
DEK forward: 5′-AGGCACTGTGTCCTCATTAA-3′;
reverse: 5′- TCTGACAGAATTTCAGGACA-3′.
c-Myc forward: 5′- TCAAGAGGCGAACACACAAC-3′;
reverse: 5′- GGCCTT- TTCATTGTTTTCCA-3′.
18S forward: 5′-CCCGGGGAGGTAGTGACGAAAAAT-3′;
reverse: 5′-CGCCCGCCCGCTCCCAAGAT-3′.
CD36 forward: 5′- TGCAAGTCCTGATGTTTCAGA -3′;
reverse: 5′- TGGCTTGACCAATAGGTTGAC -3′.
DEK forward: 5′-AGGCACTGTGTCCTCATTAA-3′;
reverse: 5′- TCTGACAGAATTTCAGGACA-3′.
c-Myc forward: 5′- TCAAGAGGCGAACACACAAC-3′;
reverse: 5′- GGCCTT- TTCATTGTTTTCCA-3′.
18S forward: 5′-CCCGGGGAGGTAGTGACGAAAAAT-3′;
reverse: 5′-CGCCCGCCCGCTCCCAAGAT-3′.