The antibiotic susceptibility test was determined as described previously [29 (link),30 (link)]. Antibiotic resistance to 13 antibiotics (KisanBio, Seoul, Korea): Ampicillin (10 µg), chloramphenicol (30 µg), clindamycin (10 µg), ciprofloxacin (5 µg), gentamicin (10 µg), doxycycline (30 µg), erythromycin (15 µg), kanamycin (30 µg), penicillin G (10 IU), streptomycin (10 µg), trimethoprim-sulfamethoxazole (25 µg), tetracycline (30 µg), and vancomycin (30 µg) were evaluated by the disc diffusion method. A total of 100 µL of LPLB5 (107 CFU/mL) was spread onto MRS agar and each antibiotic disc was placed on the plate. After incubation (at 37 °C, 24 h), the diameter of the growth inhibition zones around each disk was measured. The results were evaluated according to the Clinical and Laboratory Standards Institute (CLSI) 2012 standards [31 ].
Ampicillin
Manufactured by KisanBio
Ampicillin is a broad-spectrum antibiotic commonly used in laboratory settings. It is a beta-lactam antibiotic that inhibits the synthesis of the bacterial cell wall, effectively preventing the growth and replication of various bacteria.
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Lab products found in correlation
3 protocols using ampicillin
1
Antibiotic Susceptibility of LPLB5 Strain
2
Antibiotic Susceptibility of Lactobacillus plantarum
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Antibiotic susceptibility of the Lpb. plantarum strains was investigated as described previously [19 (link)]. A total of 13 antibiotics were used to evaluate antibiotic resistance: ampicillin (AMP; 10 μg), chloramphenicol (C; 30 μg), clindamycin (CD; 10 μg), ciprofloxacin (CIP; 5 μg), gentamicin (CN; 10 μg), doxycycline (DXT; 30 μg), erythromycin (E; 15 μg), kanamycin (K; 30 μg), penicillin G (P; 10 IU), streptomycin (S; 10 μg), trimethoprim-sulfamethoxazole (SXT; 25 μg), tetracycline (TE; 30 μg), and vancomycin (VA; 30 μg) (KisanBio, Seoul, Korea). Each Lpb. plantarum strain (1 × 107 CFU/mL; 100 μL) was spread onto MRS agar plates, and antibiotic discs were placed on the plates. After incubation at 37 °C for 24 h, the diameter of the inhibition zone was measured. The results were presented according to the Clinical and Laboratory Standards Institute 2012 standards [20 ].
3
Antibacterial Efficacy of Essential Oils
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EOs were serially diluted in NB to two final concentrations: 0.5% (v/v) and 1% (v/v).
Bacterial suspensions were dispensed into 96-well plates (100 µL/well), and 100 µL of an EO 0.5% (v/v) or 1% (v/v) EO was also dispensed in each well. The control group had 8 µg/mL ampicillin (Kisan Bio, Korea) added (instead of any EO) according to a sensitivity criterion. The cells were incubated for 18 h at 37℃.
Bacterial suspensions were dispensed into 96-well plates (100 µL/well), and 100 µL of an EO 0.5% (v/v) or 1% (v/v) EO was also dispensed in each well. The control group had 8 µg/mL ampicillin (Kisan Bio, Korea) added (instead of any EO) according to a sensitivity criterion. The cells were incubated for 18 h at 37℃.
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