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Salicylic acid

Manufactured by Tokyo Chemical Industry
Sourced in Japan

Salicylic acid is a white crystalline compound with the chemical formula C6H4(OH)COOH. It is a commonly used laboratory reagent and is known for its role as a precursor in the synthesis of various organic compounds.

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2 protocols using salicylic acid

1

Synthesis and Characterization of Nitrite Salts

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DpS, salicylic acid (SA), sodium nitrite (NaNO2), sulfamic acid, and 2,4-dinitrobenzene sulfonic acid (DNBS) were purchased from the Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Sulfasalazine (SSZ) was purchased from Sigma-Aldrich Chemical Co. Inc. (St. Louis, MO, USA). Reaction solvents and high-performance liquid chromatography (HPLC) grade solvents were obtained from Junsei Chemical Co. (Tokyo, Japan) and Daejung Chemicals & Metals Co. Ltd. (Gyeonggi-do, Siheung, Korea), respectively. Cytokine-induced neutrophil chemoattractant-3 (CINC-3) enzyme-linked immunosorbent assay (ELISA) kit was purchased from R&D Systems Inc. (Minneapolis, MN, USA). Phosphate buffer saline (pH 7.4) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals used were commercially available reagent-grade reagents. Spots on thin-layer chromatography (TLC) plates (silica gel F254s, Merck Millipore, Burlington, MA, USA) were detected using an ultraviolet lamp (254 nm). Infrared (IR) and 1H-NMR spectra were taken by a Varian FT-IR spectrophotometer (Varian Medical Systems, Palo Alto, CA, USA) and a Varian AS 500 NMR spectrophotometer (Varian Medical Systems, Palo Alto, CA, USA), respectively. The chemical shift in the NMR spectra is presented in ppm, downfield from that of tetramethylsilane.
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2

Quantification of Salicylate Metabolites

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ASA, Salicylic acid (SA), Gentisic acid (GA), 2,3-dihydroxybenzoic acid (2,3-DHBA), Salicyluric acid (SUA) and o-Toluic acid (o-TA) were purchased from Tokyo Chemical Industry Co., Ltd. (Chuo-ku, Tokyo, Japan). AcetylSalicylic acid-d4 (ASA-d4) and Gentisic acid-d3 (GA-d3) were purchased from Toronto Research Chemicals (North York, ON, Canada). Salicylic acid-d4 (SA-d4) was purchased from Sigma-Aldrich Co. LLC (St. Louis, MO, USA). Acetonitrile (LC-MS grade) was purchased from Kanto chemical (Chuo-ku, Tokyo, Japan), Ammonium Acetate Solution (HPLC grade) was purchased from FUJIFILM Wako Pure Chemical Corporation (Chuo-ku, Osaka, Japan) and Formic acid (LC-MS grade) was purchased from Honeywell Fluka™ (Morris Plains, NJ, USA).
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