DpS, salicylic acid (SA), sodium nitrite (NaNO2), sulfamic acid, and 2,4-dinitrobenzene sulfonic acid (DNBS) were purchased from the Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Sulfasalazine (SSZ) was purchased from Sigma-Aldrich Chemical Co. Inc. (St. Louis, MO, USA). Reaction solvents and high-performance liquid chromatography (HPLC) grade solvents were obtained from Junsei Chemical Co. (Tokyo, Japan) and Daejung Chemicals & Metals Co. Ltd. (Gyeonggi-do, Siheung, Korea), respectively. Cytokine-induced neutrophil chemoattractant-3 (CINC-3) enzyme-linked immunosorbent assay (ELISA) kit was purchased from R&D Systems Inc. (Minneapolis, MN, USA). Phosphate buffer saline (pH 7.4) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals used were commercially available reagent-grade reagents. Spots on thin-layer chromatography (TLC) plates (silica gel F254s, Merck Millipore, Burlington, MA, USA) were detected using an ultraviolet lamp (254 nm). Infrared (IR) and 1H-NMR spectra were taken by a Varian FT-IR spectrophotometer (Varian Medical Systems, Palo Alto, CA, USA) and a Varian AS 500 NMR spectrophotometer (Varian Medical Systems, Palo Alto, CA, USA), respectively. The chemical shift in the NMR spectra is presented in ppm, downfield from that of tetramethylsilane.
Salicylic acid
Manufactured by Tokyo Chemical Industry
Sourced in Japan
Salicylic acid is a white crystalline compound with the chemical formula C6H4(OH)COOH. It is a commonly used laboratory reagent and is known for its role as a precursor in the synthesis of various organic compounds.
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2 protocols using salicylic acid
1
Synthesis and Characterization of Nitrite Salts
2
Quantification of Salicylate Metabolites
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ASA, Salicylic acid (SA), Gentisic acid (GA), 2,3-dihydroxybenzoic acid (2,3-DHBA), Salicyluric acid (SUA) and o-Toluic acid (o-TA) were purchased from Tokyo Chemical Industry Co., Ltd. (Chuo-ku, Tokyo, Japan). AcetylSalicylic acid-d4 (ASA-d4) and Gentisic acid-d3 (GA-d3) were purchased from Toronto Research Chemicals (North York, ON, Canada). Salicylic acid-d4 (SA-d4) was purchased from Sigma-Aldrich Co. LLC (St. Louis, MO, USA). Acetonitrile (LC-MS grade) was purchased from Kanto chemical (Chuo-ku, Tokyo, Japan), Ammonium Acetate Solution (HPLC grade) was purchased from FUJIFILM Wako Pure Chemical Corporation (Chuo-ku, Osaka, Japan) and Formic acid (LC-MS grade) was purchased from Honeywell Fluka™ (Morris Plains, NJ, USA).
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