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5α cholestanol

Manufactured by Merck Group
Sourced in China

5α-cholestanol is a chemical compound used as a laboratory standard and reference material. It is a saturated steroid alcohol with the molecular formula C27H48O. 5α-cholestanol serves as a reference standard for analytical techniques such as chromatography and spectroscopy, allowing for the identification and quantification of similar compounds in research and quality control applications.

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2 protocols using 5α cholestanol

1

Lipophilic Compound Extraction Protocol

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Acetone, acetonitrile, isopropanol, ethanol, methanol, and n-hexane, (all HPLC grade) were purchased from Sigma–Aldrich (Milano, Italia). Water was purified with a Milli-Q system (Millipore, Bedford, MA, USA). All other reagents were of analytical grade. 5α-cholestanol, tocopherol (α, γ and δ-tocopherols), and phenolic acid (tyrosol and hydroxytyrosol) standards were purchased from Sigma–Aldrich (Milano, Italia).
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2

Flaxseed Extraction and Characterization

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Flaxseed was obtained from the Gansu Academy of Agricultural Sciences, China. The raw material was cleaned to remove dust, stone, other seeds, etc. The cleaned seed was dried at 50℃ until reducing to the moisture of approximately 6%. The dried sample was ground in a blender and then pass through a 30-mesh sieve. The ground seed was sealed and stored in the dark at 4℃ until use.
n-Butane solvent (99.5%) was obtained from Longyu Chemical Co., Ltd. (Puyang, China) . Fatty acids and phytosterols standards ( Campesterol, Stigmasterol, β-Sitosterol, Sitostanol and 5α-cholestanol) were purchased from Sigma-Aldrich Chemical Co., Ltd. (Shanghai, China) . All other chemicals used in study were at least of analytical grade.
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