Cells were stained with the following antibodies obtained from BD Biosciences (Franklin Lakes, NJ, USA), eBioscience, or BioLegend (San Diego, CA, USA): anti-CD4 (BioLegend, 100451), anti-CXCR3 (eBioscience, 12-1831-82), anti-CCR7 (eBioscience, 12-1971-82), anti-CD44 (eBioscience, 12-0441-83), anti-CD62L (BioLegend, 104406), anti-CD11c (BD Bioscience, 553801), anti-MHCII (BioLegend, 107631), anti-CD80 (BD Biosciences, 553769), anti-CD86 (BD Biosciences, 553692), anti-B220 (eBioscience, 12-0452-83), anti-CD8 (BD Bioscience, 553032), anti-CD103 (BD Bioscience, 557495), anti-CD45 (BioLegend, 103132), and anti-CD11b (BioLegend, 101263). For Th1 and Treg analyses, cells were stained for surface markers, permeabilized with the Intracellular Fixation and Permeabilization Buffer Set (eBioscience, 88-8824-00), and then stained with anti-IFN-γ (BioLegend, 505825) and anti-FOXP3 (eBioscience, 17-5773-82) antibodies. The following antibodies were obtained from Cell Signaling Technology (Danvers, Massachusetts, USA): anti-Ezh2 (#5246), anti-Runx1 (#4336), and anti-H3K27me3 (#9733). The Alexa Fluor™ 488 Goat Anti-Rabbit SFX Kit from Invitrogen (Carlsbad, CA, USA) was used as a secondary antibody. Multicolor flow cytometric analysis was performed using a CytoFLEX LX (Beckman Coulter, Indianapolis, IN, USA).
Anti cxcr3
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-CXCR3 is a reagent used in flow cytometry applications. It binds to the CXCR3 cell surface receptor, which is expressed on certain immune cells. This reagent can be used to identify and quantify CXCR3-expressing cells in research samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti ccr7, by Thermo Fisher Scientific (2 mentions)
Intracellular fixation and permeabilization buffer set, by Thermo Fisher Scientific (1 mentions)
Anti b220, by Thermo Fisher Scientific (1 mentions)
Anti cd45, by BioLegend (1 mentions)
Anti cd44, by Thermo Fisher Scientific (1 mentions)
Anti foxp3, by Thermo Fisher Scientific (1 mentions)
Anti runx1, by Cell Signaling Technology (1 mentions)
Anti mhcii, by BioLegend (1 mentions)
Anti cd103, by BD (1 mentions)
Alexa fluor 488 goat anti rabbit sfx kit, by Thermo Fisher Scientific (1 mentions)
Anti cd11c, by BD (1 mentions)
Anti cd8, by BD (1 mentions)
Cytoflex lx, by Beckman Coulter (1 mentions)
Anti h3k27me3, by Cell Signaling Technology (1 mentions)
Anti cd86, by BD (1 mentions)
Anti cd80, by BD (1 mentions)
Anti ezh2, by Cell Signaling Technology (1 mentions)
Anti cd4, by BioLegend (1 mentions)
Anti cd11b, by BioLegend (1 mentions)
Anti ifn γ, by BioLegend (1 mentions)
2 protocols using anti cxcr3
1
Multicolor Flow Cytometry Panel for Immune Profiling
2
Phenotypic Analysis of Senescent and Exhausted T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Peripheral blood mononuclear cells (PBMCs) were isolated using coll-paque (GE Healthcare, USA) for cellular phenotypic analysis of senescent and exhausted T cells by ow cytometry. For analysis of surface markers, cells were stained in PBS containing 2% fetal bovine serum (FBS, Thermo sher, USA)
with antibodies as indicated. Then ow cytometric analysis was carried out in BD LSRFortessa X20. The gating strategy to identify T cell subsets was applied as described previously 28 . Brie y, markers related to T cell senescence and exhaustion were also monitored using PD-1 TIM3 CD28 and CD57 antibodies.
Antibodies used in this study were from BD Biosciences and eBioscience, including anti-CD4 (GK1.5, 1:100), anti-CD8 (53-6.7, 1:100), anti-CD25 (PC61, 1:100), anti-CD45RA (HI100, 1:100), anti-CXCR3 (G025H7, 1:100), anti-CCR4 (L291H4, 1:100), anti-CCR6 (G034E3, 1:100), anti-CCR7 (G043H7, 1:100), anti-CD127 (A019D5, 1:100), anti-CXCR5 (RF8B2, 1:100), anti-CD28 (CD28.2, 1:100), anti-CD57 (NK-1, 1:100), anti-KLRG1 (2F1, 1:100), anti-PD-1 (EH12.2H7, 1:100), anti-TIM3 (F38-2E2, 1:100).
with antibodies as indicated. Then ow cytometric analysis was carried out in BD LSRFortessa X20. The gating strategy to identify T cell subsets was applied as described previously 28 . Brie y, markers related to T cell senescence and exhaustion were also monitored using PD-1 TIM3 CD28 and CD57 antibodies.
Antibodies used in this study were from BD Biosciences and eBioscience, including anti-CD4 (GK1.5, 1:100), anti-CD8 (53-6.7, 1:100), anti-CD25 (PC61, 1:100), anti-CD45RA (HI100, 1:100), anti-CXCR3 (G025H7, 1:100), anti-CCR4 (L291H4, 1:100), anti-CCR6 (G034E3, 1:100), anti-CCR7 (G043H7, 1:100), anti-CD127 (A019D5, 1:100), anti-CXCR5 (RF8B2, 1:100), anti-CD28 (CD28.2, 1:100), anti-CD57 (NK-1, 1:100), anti-KLRG1 (2F1, 1:100), anti-PD-1 (EH12.2H7, 1:100), anti-TIM3 (F38-2E2, 1:100).
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