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Chonblock

Manufactured by Chondrex
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Sourced in United States

ChonBlock is a versatile laboratory equipment designed for sample processing and preparation. It is capable of maintaining consistent temperature conditions for various applications.

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Lab products found in correlation

Maxisorp, by Thermo Fisher Scientific (5 mentions) Synergy ht microplate reader, by Agilent Technologies (5 mentions) 1 step turbo tmb elisa substrate solution, by Thermo Fisher Scientific (4 mentions) Hrp goat anti mouse, by BioLegend (4 mentions) Elx 405 bio tex plate washer, by Chondrex (2 mentions) Anti β tubulin antibody, by Santa Cruz Biotechnology (1 mentions) Chemidoc xrs system, by Bio-Rad (1 mentions)

Close spelling variants of this product

ChonBlock blocking buffer Chonblock blocking/sample dilution ELISA buffer Chonblock buffer ChonBlock detection antibody dilution buffer ChonBlock blocking/sample dilution buffer

7 protocols using chonblock

1

SARS-CoV-2 Antibody Detection by ELISA

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IgG ELISAs against hCoV strains were performed. Streptavidin solution (5 µg ml−1) was plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 50 mM sodium bicarbonate pH 8.75. This was left to incubate for 1 hour at room temperature. These were washed 3× with 300 µl of ddH2O using an ELx 405 Bio-Tex plate washer and blocked with 150 ul of ChonBlock (Chondrex) for at least 1 hour at room temperature. Biotinylated hCoV spike proteins were added to each well at a concentration of 1 µg ml−1 and left to incubate overnight at 4 °C. Plates were washed 3× with 300 µl of 1× PBST, and serial dilutions of monoclonal antibodies (described above) were added, starting at 1 µM and undergoing ten-fold serial dilutions. These were left to incubate for 1 hour at room temperature and then washed 3× with PBST. Goat anti-human HRP (Abcam, ab7153) was added at a 1:5,000 dilution in PBST. This was left to incubate at room temperature for 1 hour and then washed 6× with PBST. Finally, the plate was developed using 50 µl of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well, and the plates were quenched with 50 µl of 2 M H2SO4 to each well. Plates were read at 450 nm and normalized for path length using a BioTek Synergy HT Microplate Reader.
Weidenbacher P.A., Waltari E., de los Rios Kobara I., Bell B.N., Morris M.K., Cheng Y.C., Hanson C., Pak J.E, & Kim P.S. (2022). Converting non-neutralizing SARS-CoV-2 antibodies into broad-spectrum inhibitors. Nature Chemical Biology, 18(11), 1270-1276.
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2

SARS-CoV-2 Spike Protein ELISA

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Recombinant trimeric spike proteins (5 μg/mL) were plated in 50 μl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature. These were washed 3 times with 300 μl of ddH2O using an ELx 405 Bio-Tex plate washer and blocked with 150 μl of ChonBlock (Chondrex) for at least 1 h at room temperature. Plates were washed 3x with 300 μl of 1x PBST. Mouse serum samples, serially diluted in diluent buffer (1x PBS, 0.1% Tween) starting at 1:50 serum dilution was then added to coated plates for 1 h at room temperature. This was then washed 3x with PBST. HRP goat anti-mouse (BioLegend 405306) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. This was left to incubate at room temperature for 1 h and then washed 6x with PBST. Finally, the plate was developed using 50 μl of 1-Step TMB Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well, and the plates were quenched with 50 μl of 2 M H2SO4 to each well. Plates were read at 450 nm and normalized for path length using a BioTek Synergy HT Microplate Reader.
Weidenbacher P.A., Friedland N., Sanyal M., Morris M.K., Do J., Hanson C, & Kim P.S. (2023). Decreased efficacy of a COVID-19 vaccine due to mutations present in early SARS-CoV-2 variants of concern. bioRxiv.
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3

ELISA for Measuring Mouse RBD Antibodies

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RBD (5 µg/mL) was plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature. These were washed 3 times with 300 µl of ddH2O using an ELx 405 Bio-Tex plate washer and blocked with 150 µl of ChonBlock (Chondrex) for at least 1 h at room temperature. Plates were washed 3x with 300 µl of 1x PBST. Mouse serum samples, serially diluted in diluent buffer (1x PBS, 0.1% Tween) starting at 1:50 serum dilution was then added to coated plates for 1 h at room temperature. This was then washed 3x with PBST. HRP goat anti-mouse (BioLegend 405306) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. This was left to incubate at room temperature for 1 h and then washed 6x with PBST. Finally, the plate was developed using 50 µl of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well, and the plates were quenched with 50 µl of 2 M H2SO4 to each well. Plates were read at 450 nm and normalized for path length using a BioTek Synergy HT Microplate Reader.
Weidenbacher P.A., Sanyal M., Friedland N., Tang S., Arunachalam P.S., Hu M., Kumru O.S., Morris M.K., Fontenot J., Shirreff L., Do J., Cheng Y.C., Vasudevan G., Feinberg M.B., Villinger F.J., Hanson C., Joshi S.B., Volkin D.B., Pulendran B, & Kim P.S. (2023). A ferritin-based COVID-19 nanoparticle vaccine that elicits robust, durable, broad-spectrum neutralizing antisera in non-human primates. Nature Communications, 14, 2149.
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4

Immunoblotting Analysis of Kidney Proteins

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15 μg of soluble protein extracted from isolated glomeruli or whole kidney lysates were prepared for electrophoresis on 10% SDS-PAGE. After transfer to nitrocellulose paper and blocking, immunostaining was performed in either 5% milk or BSA in TBST buffer or ChonBlock (Chondrex, USA). Primary and secondary antibodies are listed in Supp. Table1. Secondary antibodies, either goat anti-mouse or donkey anti-rabbit (Santa Cruz Biotechnology) were labeled with peroxidase. Proteins were detected using the Enhanced ChemiLuminescence Plus Blotting Detection system (Amersham Biosciences, UK) and were visualized through the ChemiDoc™ XRS+ System (BioRad, CA, USA). All transblots were reprobed with anti–β-tubulin antibody (Santa Cruz Biotechnology) (1:500), for normalization. Band density was defined with the ImageJ Software (http://imagej.nih.gov/ij).
Odiatis C., Savva I., Pieri M., Ioannou P., Petrou P., Papagregoriou G., Antoniadou K., Makrides N., Stefanou C., Ljubanović D.G., Nikolaou G., Borza D.B., Stylianou K., Gross O, & Deltas C. (2020). A glycine substitution in the collagenous domain of Col4a3 in mice recapitulates late onset Alport syndrome. Matrix Biology Plus, 9, 100053.
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5

Biotinylated Peptide Binding Assay

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For experiments with the biotinylated peptides, Nunc Immobilizer Strepavidin 384 plates (Thermo Fisher) were loaded with 1 μM of biotinylated peptide (table S8) for 1 hour at room temperature. Plates were blocked with 50% Chonblock (Chondrex) in PBS (Chondrex) or 2% BSA in PBS for 1 hour at room temperature. Following blocking, plasma or synovial fluid was diluted at 1:100 concentration or to 1 μg/mL for mAbs, and was incubated shaking at room temperature for 1.5 hours. Dilution series of a positive control sera was included on each plate to ensure consistency between plates. Antibody binding was detected by using an HRP-conjugated goat anti-human IgG Fc antibody and Super AquaBlue ELISA substrate. Five washes with PBST were performed between each step. Blank wells were subtracted for analysis.
Faruque S.M., Rahman M.M., A.s.a.d.u.l.g.h.a.n.i., Islam K.M, & Mekalanos J.J. (1999). Lysogenic Conversion of Environmental Vibrio mimicus Strains by CTXΦ. Infection and Immunity, 67(11), 5723-5729.
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6

ELISA for Measuring Mouse RBD Antibodies

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RBD (5 µg/mL) was plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature. These were washed 3 times with 300 µl of ddH2O using an ELx 405 Bio-Tex plate washer and blocked with 150 µl of ChonBlock (Chondrex) for at least 1 h at room temperature. Plates were washed 3x with 300 µl of 1x PBST. Mouse serum samples, serially diluted in diluent buffer (1x PBS, 0.1% Tween) starting at 1:50 serum dilution was then added to coated plates for 1 h at room temperature. This was then washed 3x with PBST. HRP goat anti-mouse (BioLegend 405306) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. This was left to incubate at room temperature for 1 h and then washed 6x with PBST. Finally, the plate was developed using 50 µl of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well, and the plates were quenched with 50 µl of 2 M H2SO4 to each well. Plates were read at 450 nm and normalized for path length using a BioTek Synergy HT Microplate Reader.
Weidenbacher P.A., Sanyal M., Friedland N., Tang S., Arunachalam P.S., Hu M., Kumru O.S., Morris M.K., Fontenot J., Shirreff L., Do J., Cheng Y.C., Vasudevan G., Feinberg M.B., Villinger F.J., Hanson C., Joshi S.B., Volkin D.B., Pulendran B, & Kim P.S. (2023). A ferritin-based COVID-19 nanoparticle vaccine that elicits robust, durable, broad-spectrum neutralizing antisera in non-human primates. Nature Communications, 14, 2149.
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7

ELISA for Measuring Mouse RBD Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
RBD (5 µg/mL) was plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature. These were washed 3 times with 300 µl of ddH2O using an ELx 405 Bio-Tex plate washer and blocked with 150 µl of ChonBlock (Chondrex) for at least 1 h at room temperature. Plates were washed 3x with 300 µl of 1x PBST. Mouse serum samples, serially diluted in diluent buffer (1x PBS, 0.1% Tween) starting at 1:50 serum dilution was then added to coated plates for 1 h at room temperature. This was then washed 3x with PBST. HRP goat anti-mouse (BioLegend 405306) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. This was left to incubate at room temperature for 1 h and then washed 6x with PBST. Finally, the plate was developed using 50 µl of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well, and the plates were quenched with 50 µl of 2 M H2SO4 to each well. Plates were read at 450 nm and normalized for path length using a BioTek Synergy HT Microplate Reader.
Weidenbacher P.A., Sanyal M., Friedland N., Tang S., Arunachalam P.S., Hu M., Kumru O.S., Morris M.K., Fontenot J., Shirreff L., Do J., Cheng Y.C., Vasudevan G., Feinberg M.B., Villinger F.J., Hanson C., Joshi S.B., Volkin D.B., Pulendran B, & Kim P.S. (2023). A ferritin-based COVID-19 nanoparticle vaccine that elicits robust, durable, broad-spectrum neutralizing antisera in non-human primates. Nature Communications, 14, 2149.
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