The following antibodies were obtained from commercial sources as indicated: mouse anti-Myc (Cell Signaling Technology); rabbit and mouse anti-GFP (Invitrogen and Roche); mouse anti-ATP synthase subunit alpha (CV), mouse anti-complex IV subunit 1, mouse anti-complex I subunit NDUFS3 (CI), mouse anti-complex I subunit NDUFA9 (CI) (Mitosciences, Abcam); mouse anti-actin, mouse anti-tubulin (Sigma). Secondary horseradish peroxidase-conjugated anti-mouse and anti-rabbit antibodies were purchased from Pierce.
Rabbit and mouse anti gfp
Manufactured by Thermo Fisher Scientific
Rabbit and mouse anti-GFP are primary antibodies used to detect and visualize Green Fluorescent Protein (GFP) in biological samples. They specifically bind to GFP, enabling the identification and localization of GFP-tagged proteins or cells.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Rabbit anti hrp, by Jackson ImmunoResearch (2 mentions)
Mouse anti h3k4me3, by Thermo Fisher Scientific (2 mentions)
Mouse anti fas2, by Developmental Studies Hybridoma Bank (2 mentions)
Rabbit anti dsred, by Thermo Fisher Scientific (2 mentions)
Mouse anti myc, by Cell Signaling Technology (1 mentions)
4 protocols using rabbit and mouse anti gfp
1
Antibody Validation for Protein Analysis
2
Immunocytochemistry of Drosophila Insulin-Like Peptides
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For immunocytochemistry adult Drosophila heads were dissected in 0.1 M sodium phosphate buffer (PB), pH 7.4 and fixed in ice-cold 4% paraformaldehyde in 0.1 M PB for 2-4 h and dissected adult brains were used for whole mount immunocytochemistry.
Incubation with primary antiserum for whole mount tissues was performed for 48 h at 4°C. The following primary antisera were used: rabbit antiserum to Drosophila insulin-like peptide 2 (anti-DILP2) at 1∶4000 (gift from M. Brown, Athens, GA), rabbit and mouse anti-GFP (Invitrogen) were used at 1∶1000. For detection of primary antisera Cy3-tagged goat anti-rabbit antiserum (Jackson Immuno Research) and Alexa-488 tagged goat anti-mouse (Invitrogen) were used at 1∶1000.
Incubation with primary antiserum for whole mount tissues was performed for 48 h at 4°C. The following primary antisera were used: rabbit antiserum to Drosophila insulin-like peptide 2 (anti-DILP2) at 1∶4000 (gift from M. Brown, Athens, GA), rabbit and mouse anti-GFP (Invitrogen) were used at 1∶1000. For detection of primary antisera Cy3-tagged goat anti-rabbit antiserum (Jackson Immuno Research) and Alexa-488 tagged goat anti-mouse (Invitrogen) were used at 1∶1000.
3
Immunolabeling of Late Embryos
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The following antibodies were used for immunolabelling of late stage embryos and chromosomal preparations: mouse anti-Fas2 (Developmental Studies Hybridoma Bank, DSHB), rabbit anti-HRP (Jackson Immunoresearch), mouse anti-Connectin (DSHB), rabbit anti-dsRED (Invitrogen), rabbit and mouse anti-GFP (Invitrogen), mouse anti-H3K4me3 (Invitrogen). Secondary antibodies were purchased from Invitrogen. DNA was labeled with DAPI (Invitrogen). Embryos were collected and fixed via a formaldehyde/MeOH method10 (link). Polytene chromosome preparations and staining were performed as in Karachentsev et al.27 (link). Images of the ventral nerve cord were obtained using a Leica SP8 using a 40x Objective. Fas2 and HRP labeled embryos were imaged and typically contained 8–10 hemisegments. Hemisegments were examined for midline crossing and in some instances the presence or integrity of the most lateral Fas2+ longitudinal track. Similar imaging and analysis were performed on Connectin/HRP labeled embryos.
4
Immunolabeling and Imaging of Drosophila Embryos
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The following antibodies were used for immunolabelling of late stage embryos and chromosomal preparations: mouse anti-Fas2 (Developmental Studies Hybridoma Bank, DSHB), rabbit anti-HRP (Jackson Immunoresearch), mouse anti-Connectin (DSHB), rabbit anti-dsRED (Invitrogen), rabbit and mouse anti-GFP (Invitrogen), mouse anti-H3K4me3 (Invitrogen). Secondary antibodies were purchased from Invitrogen. DNA was labeled with DAPI (Invitrogen). Embryos were collected and fixed via a formaldehyde/MeOH method [13 (link)]. Polytene chromosome preparations and staining were performed as in Karachentsev et al. [21 (link)]. Images of the ventral nerve cord were obtained using a Leica SP8 using a 40x Objective. Fas2 and HRP labeled embryos were imaged and typically contained 8–10 hemisegments. Hemisegments were examined for midline crossing and in some instances the presence or integrity of the most lateral Fas2+ longitudinal track. Similar imaging and analysis were performed on Connectin/HRP labeled embryos.
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