Human ctgf ccn2 duoset elisa kit

Atractylenolide-I (AT-I, purity > 98%) and paclitaxel (purity > 98%) were purchased from Selleck Chemicals (Houston, TX, USA). A human CTGF/CCN2 DuoSet ELISA kit and recombinant human CTGF protein (rCTGF) were purchased from R&D Systems (Minneapolis, MN, USA). AT-1 was used at a dose of 0-100 μM (17 (link)). rCTGF was used at a dose of 0-20 μg/mL (38 ). TRIzol reagent was bought from Invitrogen (Carlsbad, CA, USA). M-MLV reverse transcriptase was bought from Promega (Madison, WI, USA). SYBR Green Real-time PCR Master Mix was bought from TOYOBO (Osaka, Osaka Prefecture, Japan). Puromycin was bought from Coolaber Technologies (Beijing, China). Anti-CTGF, anti-αSMA and anti-FAP antibodies were obtained from Abcam (Cambridge, UK). Anti-β-tubulin antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HRP-conjugated goat anti-rabbit IgG and goat anti-mouse IgG antibodies were obtained from OriGene Technologies (Rockville, MD, USA). Immobilon Western Chemiluminescent HRP Substrate was bought from Millipore (Billerica, MA, USA).

The Human Pro-Collagen I α1 DuoSet ELISA kit (R&D systems) and the Human CTGF/CCN2 DuoSet ELISA kit (R&D systems) were used according to manufacturer’s protocol to determine pro-collagen I α1 and CTGF concentrations in the culture medium, respectively. The medium samples were centrifuged immediately after harvesting at 2000× g for 10 min to remove debris and the supernatant was stored at −20 °C until further use.