Monocytes were isolated from buffy coats using Ficoll centrifugation (Lymphoprep; Axis Shield) after negative selection using the RosetteSep Monocyte Enrichment Kit (StemCell Technologies). Monocyte-derived DC (MoDC) were obtained after 6 days of differentiation in complete RPMI medium (RPMI 1,640, 1 mM sodium pyruvate, 10 mM HEPES, 2 mM L-glutamine, 1% Penicillin/Streptomycin, Hyclone GE Healthcare, and 10% FBS, Sigma) complemented with GM-CSF (250 ng/mL; PeproTech) and rIL-4 (6.5 ng/mL; R&D Systems). Cells were seeded at a density of 5 × 105 cells/mL and after 3 days of differentiation, 50% of the medium was replaced and new cytokines added. Staining for flow cytometry was done before and at indicated time points post viral infection. MoDC were incubated with LIVE/DEAD® Fixable near-IR Dead Cell Stain Kit (Life Technologies) followed by staining with CD14-PE-Cy7 (MφP9), CD1a-BV510 (HI149), CD80-PE (L307.4), and CD86-APC (2331 FUN-1) from BD Biosciences. The mouse anti-IAV NP mAb (H16-L10-4R5; Merck Millipore) was detected with a secondary Ab coupled to Alexa Fluor 488 fluorochrome with the Zenon® Kit (Invitrogen). Acquisition was done on a Fortessa flow cytometer (BD Biosciences) and analysis was performed with FlowJo software (Tree Star, version 10.2).
Cd80 pe l307.4
Manufactured by BD
CD80-PE (L307.4) is a lab equipment product. It is a fluorescently labeled antibody that binds to the CD80 cell surface protein.
Automatically generated - may contain errors
Lab products found in correlation
Live dead fixable near ir dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
Gm csf, by Thermo Fisher Scientific (1 mentions)
Fortessa flow cytometer, by BD (1 mentions)
Ril 4, by R&D Systems (1 mentions)
Zenon kit, by Thermo Fisher Scientific (1 mentions)
Rosettesep monocyte enrichment kit, by STEMCELL (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Lymphoprep, by Axis-Shield (1 mentions)
Penicillin streptomycin, by GE Healthcare (1 mentions)
Fab1298p, by R&D Systems (1 mentions)
Cd69 fitc fn50, by BD (1 mentions)
Cd3 percp sk7, by BD (1 mentions)
Cd8 apc rpa t8, by BD (1 mentions)
Cd56 pe, by BD (1 mentions)
2 protocols using cd80 pe l307.4
1
Monocyte-derived Dendritic Cell Differentiation
2
Multiparametric Phenotyping of Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following flow cytometry antibodies were used: CD11c APC-Vio770 (MJ4-27G12, Miltenyi Biotec), CD14-PerCP (TUK4, Miltenyi Biotec), CD86-phycoerythrin (PE)-Cy7 (2331, BD Biosciences), CD80-PE (L307.4, BD Biosciences), HLA-ABC-VioBlue (REA230, Miltenyi Biotec), HLA-DR/DP/DQ-fluorescein isothiocyanate (FITC) (Tu39, BD Biosciences), CD3-PerCP (SK7, BD Biosciences), CD56-PE (B159, BD Biosciences), CD8-APC (RPA-T8, BD Biosciences) CD107a-FITC (H4A3, BD Biosciences), CD107b-FITC (H4B4, BD Biosciences), CD69-FITC (FN50, BD Biosciences), MICA/B-PE (6D4, BD Biosciences), ULBP2/5/6 (FAB1298p, R&D Systems), IFNγ-BV421 (4S.B3, BD Biosciences), mouse immunoglobulin G1 (IgG1) κ isotype control (PE/FITC/PerCP/PE-Cy7/APC; MOPC-21, BD Biosciences), mouse IgG2a κ isotype control (FITC/PE; G155-178, BD Biosciences), and REA Control-VioBlue (REA293, Miltenyi Biotec).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!