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Superscript 3 reagent kit

Manufactured by Thermo Fisher Scientific
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The SuperScript III reagent kit is a reverse transcription system designed for the synthesis of first-strand cDNA from RNA templates. The kit includes reagents necessary for the conversion of RNA into cDNA, which can be used in various downstream applications.

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Lab products found in correlation

Powerup sybr green master mix, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Iq sybr green supermix, by Bio-Rad (1 mentions)

Close spelling variants of this product

Superscript III (4906 citations) SuperScript III kit (807 citations) SuperScript III reagents (33 citations) Superscript reagents (8 citations) SuperScript Reagent Kit (6 citations)

2 protocols using superscript 3 reagent kit

1

Comprehensive Gene Expression Analysis

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Total RNA was isolated using an RNeasy mini kit (Qiagen Hilden, Germany). First-strand cDNA synthesis was performed using a SuperScript III reagent kit (Invitrogen, Carlsbad, CA, USA). Real-time PCR was then carried out with primers specific for ANKDR1, CTGF, CYR61, YAP, TAZ, TPC2, ORAI1, STIM1, PKC-βII, INF2, ZEB1, VIMENTIN, CCND1, CCNB1, and p21 (Table 1), using a Powerup SYBR green master mix (Applied Biosystems, Waltham, Massachusetts, USA). GAPDH and H3 were used as internal controls. Relative mRNA expression levels were calculated using the ΔΔCT method.
D’Amore A., Hanbashi A.A., Di Agostino S., Palombi F., Sacconi A., Voruganti A., Taggi M., Canipari R., Blandino G., Parrington J, & Filippini A. (2020). Loss of Two-Pore Channel 2 (TPC2) Expression Increases the Metastatic Traits of Melanoma Cells by a Mechanism Involving the Hippo Signalling Pathway and Store-Operated Calcium Entry. Cancers, 12(9), 2391.
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2

Total RNA Isolation and Gene Expression Analysis

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Total RNA was isolated with 0.5 mL of TRIZOL reagent (Invitrogen) following the manufacturer’s protocol. Reverse transcription was performed using Superscript III reagent kit from Invitrogen. All samples were checked for quality (A260/A280 ratio of 1.8–2.0). Real Time PCR was performed by using iQ SYBR Green superMix (BioRad). Gene expression levels were determined using a comparative method (DCq) normalizing the target mRNA to bactin as endogenous internal control. Forward and reverse primers sequences are listed in table S1.
Trindade B.C., Ceglia S., Berthelette A., Raso F., Howley K., Muppidi J.R, & Reboldi A. (2021). The cholesterol metabolite 25-hydroxycholesterol restricts activation of the transcriptional regulator SREBP2 and limits intestinal IgA plasma cell differentiation. Immunity, 54(10), 2273-2287.e6.
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