Log In Sign up
The largest database of trusted experimental protocols

Legendplex cloud based data analysis software

Manufactured by BioLegend
Visit Supplier

LEGENDplex Cloud-based Data Analysis Software is a data analysis tool developed by BioLegend. It is designed to streamline the analysis of data generated from flow cytometry experiments.

Automatically generated - may contain errors

Lab products found in correlation

Legendplex multiplex bead assay, by BioLegend (2 mentions) Attune nxt flow cytometer, by BioLegend (2 mentions) Legendplex, by BioLegend (2 mentions) Legendplex assay, by BioLegend (1 mentions) Human proinflammatory chemokine panel 1, by BioLegend (1 mentions) Dp400, by R&D Systems (1 mentions) Human inflammation panel 1, by BioLegend (1 mentions) Mini elisa plate reader, by BioLegend (1 mentions) Sh800, by Sony (1 mentions) Legendplex mouse anti virus response panel 13 plex, by BioLegend (1 mentions) Facs canto 2, by BioLegend (1 mentions) Legendplex human inflammation panel 1 13 plex, by BioLegend (1 mentions)

7 protocols using legendplex cloud based data analysis software

1

Cytokine and Chemokine Profiling in Blood Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Supernatants from whole-blood stimulation, monocytes and AMLs, and plasma, serum and BAL samples were assessed by ELISA to determine their IL-12p40 (#DP400, R&D Systems), IFN-γ (#DIF50, R&D Systems), CCL-2 (#438804, BioLegend), CCL-8 (#442204, BioLegend) or CCL-13 (#442004, BioLegend) content, in accordance with the kit manufacturer’s protocol. Plasma and BAL samples were also analyzed for the presence of inflammatory cytokines and chemokines with the following LEGENDplex™ assays (all from BioLegend): Human Inflammation Panel 1 (#740809), Human Inflammation Panel 2 (#740883), Human Proinflammatory Chemokine Panel 1 (#740985) and Human Proinflammatory Chemokine Panel 2 (#741183) according to the manufacturer’s instructions. Samples were analyzed by flow cytometry on a Gallios flow cytometer. Data analysis was performed with LEGENDplex Cloud-based Data Analysis Software (BioLegend). All plasma, serum and BAL samples were obtained from patients and controls at infection-free time points (culture and PCR negative).
Neehus A.L., Carey B., Landekic M., Panikulam P., Deutsch G., Ogishi M., Arango-Franco C.A., Philippot Q., Modaresi M., Mohammadzadeh I., Berndt M.C., Rinchai D., Le Voyer T., Rosain J., Momenilandi M., Martin-Fernandez M., Khan T., Bohlen J., Han J.E., Deslys A., Bernard M., Gajardo-Carrasco T., Soudée C., Le Floc’h C., Migaud M., Seeleuthner Y., Jang M.S., Nikolouli E., Seyedpour S., Begueret H., Emile J.F., Le Guen P., Tavazzi G., Julia Colombo C.N., Marzani F.C., Angelini M., Trespidi F., Ghirardello S., Alipour N., Molitor A., Carapito R., Mazloomrezaei M., Rokni-Zadeh H., Changi-Ashtiani M., Brouzes C., Vargas P., Borghesi A., Lachmann N., Bahram S., Crestani B., Pahari S., Schlesinger L.S., Marr N., Bugonovic D., Boisson-Dupuis S., Béziat V., Abel L., Borie R., Young L.R., Deterding R., Shahrooei M., Rezaei N., Parvaneh N., Craven D., Gros P., Malo D., Sepulveda F.E., Nogee L.M., Aladjidi N., Trapnell B.C., Casanova J.L, & Bustamante J. (2023). Human inherited CCR2 deficiency underlies progressive polycystic lung disease. Cell, 187(2), 390-408.e23.
+ Open protocol
+ Expand
2

Cytokine Profiling in Vaccinated Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokine levels in bronchoalveolar lavage (BAL) fluid obtained from vaccinated mice were analyzed by bead-based multiplex assay (LEGENDplexTM; BioLegend), according to manufacturer’s instruction. Samples were acquired on BD LSRFortessa Cell Analyzer and data were analyzed by LEGENDplex™ Cloud-based Data Analysis Software (BioLegend).
Yuen C.K., Wong W.M., Mak L.F., Lam J.Y., Cheung L.Y., Cheung D.T., Ng Y.Y., Lee A.C., Zhong N., Yuen K.Y, & Kok K.H. (2023). An interferon-integrated mucosal vaccine provides pan-sarbecovirus protection in small animal models. Nature Communications, 14, 6762.
+ Open protocol
+ Expand
3

Multiplex Cytokine Profiling of TLR3/MDA5 Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used a synthetic analog of dsRNA, poly(I:C), as a nonspecific agonist of TLR3 and MDA5/RIG-I. SV40-fibroblast cells were activated in 24-well plates, at a density of 100,000 cells/well, by incubation for 24 h with poly(I:C) at concentrations of 1, 5 and 25 μg/mL. Cells were stimulated with 25 μg/ml poly(I:C) in the presence of Lipofectamine 2000 to activate MDA5/RIG-I signaling. After 24 h, cell supernatants were used for the LEGENDplex multiplex bead assay (BioLegend, #740003 and #740984), then analyzed by flow cytometry on an Attune NxT Flow Cytometer, according to the manufacturer’s instructions. Data were analyzed with LEGENDplex Cloud-based Data Analysis Software (BioLegend), and presented in raw values and heatmaps. The heatmaps are generated using relative values for each sample as normalized to the maximum range of production levels of each cytokine among all samples (X-Min)/(Max-Min). Min: minimum production level; Max: maximum production level; X: the production level of a given cytokine in a given sample (96 ).
Liu Z., Garcia Reino E.J., Harschnitz O., Guo H., Chan Y.H., Khobrekar N., Hasek M.L., Dobbs K., Rinchai D., Materna M., Matuozzo D., Lee D., Bastard P., Chen J., Lee Y.S., Kim S.K., Zhao S., Amin P., Lorenzo L., Seeleuthner Y., Chevalier R., Mazzola L., Gay C., Stephan J.L., Milisavljevic B., Boucherit S., Rozenberg F., Perez de Diego R., Dix R.D., Marr N., Béziat V., Cobat A., Aubart M., Abel L., Chabrier S., Smith G.A., Notarangelo L.D., Mocarski E.S., Studer L., Casanova J.L, & Zhang S.Y. (2023). Encephalitis and poor neuronal death-mediated control of herpes simplex virus in human inherited RIPK3 deficiency. Science immunology, 8(82), eade2860.
+ Open protocol
+ Expand
4

Neutrophil Mobilization and Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokine and chemokines involved in neutrophil mobilization and activation were measured using a custom multiplex bead array kit (LEGENDPlex, Biolegend). Twelve cytokines were measured including, CXCL1, CXCL2, G-CSF, IL-1b, IL-6, IL-4, TGFb, TNFa, GM-CSF, INFg, IL-17A, and IL-10. The assay was performed according to manufacturer’s instructions in duplicate, collected on an SH800 (Sony), and analyzed using the LEGENDPlex Cloud-Based Data Analysis Software (Biolegend and Qognit). Neutrophil elastase was measured via ELISA using a pre-validated kit following manufacturer’s instructions (Abcam) and read using a Mini ELISA Plate Reader (Biolegend).
Hensley-McBain T., Aries M, & Cook M. (2023). Peripheral Low Level Chronic LPS Injection as a Model of Neutrophil Activation in the Periphery and Brain in Mice. Research Square.
+ Open protocol
+ Expand
5

Multiplex Cytokine Profiling in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma cytokines and chemokines were analyzed using LEGENDplexTM Mouse Anti-Virus Response Panel (13-plex) (BioLegend, San Diego, California, US), according to manufacturer’s instructions. Data were analyzed using LEGENDplex™ Cloud-based Data Analysis Software (BioLegend).
Das K., Belnoue E., Rossi M., Hofer T., Danklmaier S., Nolden T., Schreiber L.M., Angerer K., Kimpel J., Hoegler S., Spiesschaert B., Kenner L., von Laer D., Elbers K., Derouazi M, & Wollmann G. (2021). A modular self-adjuvanting cancer vaccine combined with an oncolytic vaccine induces potent antitumor immunity. Nature Communications, 12, 5195.
+ Open protocol
+ Expand
6

Multiplex Inflammatory Cytokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma- or serum samples were frozen and thawed for the analysis in a Legendplex (13-plex) Human Inflammation Panel 1 (Biolegend). Samples were measured in duplicates and incubated with beads at 4°C overnight and for 1 h at RT on the next day. The further work steps were performed according to the manufacturer instructions. Samples were measured on a FACS Canto II and evaluated with the LEGENDplex™ Cloud-based Data Analysis Software (Biolegend collaboration with Qognit). If the value was underneath the detection limit than the value was set equal to the lowest calculated standard value.
Wittmann N., Mishra N., Gramenz J., Kuthning D., Behrendt A.K., Bossaller L, & Meyer-Bahlburg A. (2023). Inflammasome activation and formation of ASC specks in patients with juvenile idiopathic arthritis. Frontiers in Medicine, 10, 1063772.
+ Open protocol
+ Expand
7

Multiplex Cytokine Profiling of TLR3/MDA5 Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used a synthetic analog of dsRNA, poly(I:C), as a nonspecific agonist of TLR3 and MDA5/RIG-I. SV40-fibroblast cells were activated in 24-well plates, at a density of 100,000 cells/well, by incubation for 24 h with poly(I:C) at concentrations of 1, 5 and 25 μg/mL. Cells were stimulated with 25 μg/ml poly(I:C) in the presence of Lipofectamine 2000 to activate MDA5/RIG-I signaling. After 24 h, cell supernatants were used for the LEGENDplex multiplex bead assay (BioLegend, #740003 and #740984), then analyzed by flow cytometry on an Attune NxT Flow Cytometer, according to the manufacturer’s instructions. Data were analyzed with LEGENDplex Cloud-based Data Analysis Software (BioLegend), and presented in raw values and heatmaps. The heatmaps are generated using relative values for each sample as normalized to the maximum range of production levels of each cytokine among all samples (X-Min)/(Max-Min). Min: minimum production level; Max: maximum production level; X: the production level of a given cytokine in a given sample (96 ).
Liu Z., Garcia Reino E.J., Harschnitz O., Guo H., Chan Y.H., Khobrekar N., Hasek M.L., Dobbs K., Rinchai D., Materna M., Matuozzo D., Lee D., Bastard P., Chen J., Lee Y.S., Kim S.K., Zhao S., Amin P., Lorenzo L., Seeleuthner Y., Chevalier R., Mazzola L., Gay C., Stephan J.L., Milisavljevic B., Boucherit S., Rozenberg F., Perez de Diego R., Dix R.D., Marr N., Béziat V., Cobat A., Aubart M., Abel L., Chabrier S., Smith G.A., Notarangelo L.D., Mocarski E.S., Studer L., Casanova J.L, & Zhang S.Y. (2023). Encephalitis and poor neuronal death-mediated control of herpes simplex virus in human inherited RIPK3 deficiency. Science immunology, 8(82), eade2860.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!