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Geranylgeraniol

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Geranylgeraniol is a naturally occurring diterpene alcohol. It is a key intermediate in the biosynthesis of various isoprenoid compounds, including carotenoids, chlorophyll, and certain plant hormones.

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Lab products found in correlation

Forskolin, by Thermo Fisher Scientific (2 mentions) 5977b mass, by Agilent Technologies (2 mentions) Vf 5ht, by Agilent Technologies (2 mentions) Rpmi 1640, by Merck Group (1 mentions) Hepes, by Merck Group (1 mentions) Hepes, by Corning (1 mentions) Fosmidomycin, by Merck Group (1 mentions)

3 protocols using geranylgeraniol

1

Cultivation of Parasites in Optimized Conditions

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Parasites were cultured using standard methods (56 (link)) in flasks gassed with a mixture of 90% N2, 5% CO2, and 5% O2 (Airgas; catalog no. Z03NI9022000033). Normal culture medium consisted of RPMI 1640 with l-glutamine and 25 mM HEPES (Corning; catalog no. 10-041-CV), with 5.0 g/liter Albumax II lipid-rich bovine serum albumin (BSA; Thermo Fisher Scientific; catalog no. 11021029), 3.7 mM hypoxanthine, and 50 μg/ml gentamicin. The isoleucine-deficient medium consisted of 10.3 g/liter RPMI 1640 isoleucine (Ile) Drop-out medium (United States BioLogicals; catalog no. R9014), supplemented with 2.0 g/liter NaHCO3, 6.0 g/liter HEPES, 5.0 g/liter Albumax II, 3.7 mM hypoxanthine, and 50 μg/ml gentamicin. The 8 μM isoleucine medium was the same as the isoleucine medium, except that it was supplemented with 8 μM l-isoleucine. The 20% glucose media consisted of 8.4 g/liter of no-glucose RPMI 1640 (Sigma-Aldrich; catalog no. R1383), with 2.0 g/liter NaHCO3, 6.0 g/liter HEPES, 5.0 g/liter Albumax II, 3.7 mM hypoxanthine, 50 μg/ml gentamicin, and 0.4 g/liter glucose. Fosmidomycin was obtained from Sigma-Aldrich (catalog no. F8682-5MG), and geranylgeraniol was obtained from Santa Cruz Biotechnology (catalog no. sc-200858).
McLean K.J, & Jacobs-Lorena M. (2017). Plasmodium falciparum Maf1 Confers Survival upon Amino Acid Starvation. mBio, 8(2), e02317-16.
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2

GC-MS Analysis of Geranylgeraniol and Forskolin

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Samples were analyzed using an Agilent 7820A gas chromatogram coupled to an Agilent 5977B mass spectrometer. The samples were analyzed on an Agilent VF-5HT column (30 m x 0.25 mm x 0.1 μm) using a constant flow of 1 mL/min of helium. The inlet was set at 280°C in split mode with a 10:1 split ratio. The injection volume was set to 1 μL. Oven conditions were as follows: 130°C for 2 min, ramp to 250°C at 8°C/min, ramp to 310°C at 10°C/min and hold for 5 min. The MSD transfer line was set to 250°C, the MS Source was set to 230°C, and the MS Quad was set to 150°C. MS data was collected using Scan mode from 50 −550 m/z with a 4 min solvent delay. Geranylgeraniol was purchased from Santa Cruz Biotechnology (CAS 24034–73-9). Forskolin was purchased from Fisher Scientific (CAS 66575–29-9).
De La Peña R, & Sattely E.S. (2020). Re-routing plant terpene biosynthesis enables momilactone pathway elucidation. Nature chemical biology, 17(2), 205-212.
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3

GC-MS Analysis of Geranylgeraniol and Forskolin

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Samples were analyzed using an Agilent 7820A gas chromatogram coupled to an Agilent 5977B mass spectrometer. The samples were analyzed on an Agilent VF-5HT column (30 m x 0.25 mm x 0.1 μm) using a constant flow of 1 mL/min of helium. The inlet was set at 280°C in split mode with a 10:1 split ratio. The injection volume was set to 1 μL. Oven conditions were as follows: 130°C for 2 min, ramp to 250°C at 8°C/min, ramp to 310°C at 10°C/min and hold for 5 min. The MSD transfer line was set to 250°C, the MS Source was set to 230°C, and the MS Quad was set to 150°C. MS data was collected using Scan mode from 50 −550 m/z with a 4 min solvent delay. Geranylgeraniol was purchased from Santa Cruz Biotechnology (CAS 24034–73-9). Forskolin was purchased from Fisher Scientific (CAS 66575–29-9).
De La Peña R, & Sattely E.S. (2020). Re-routing plant terpene biosynthesis enables momilactone pathway elucidation. Nature chemical biology, 17(2), 205-212.
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