Pen 2

Manufactured by Abcam

Sourced in United States

Pen-2 is a laboratory equipment designed for pipetting and dispensing liquids. It functions as a manual pipette, allowing for precise and controlled liquid handling in various laboratory applications.

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Lab products found in correlation

Aph1a, by Thermo Fisher Scientific (2 mentions) Dc protein assay kit, by Bio-Rad (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) α tubulin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Neuronal nuclei (neun), by Merck Group (1 mentions) Glur2, by Merck Group (1 mentions) Myc 9e10, by Santa Cruz Biotechnology (1 mentions) β actin, by Merck Group (1 mentions) Cleaved notch1 val1744, by Cell Signaling Technology (1 mentions) Goat anti mouse igg, by Santa Cruz Biotechnology (1 mentions) P ser199, by Abcam (1 mentions) P thr205, by Abcam (1 mentions) P thr231, by Abcam (1 mentions) P ser396, by Abcam (1 mentions) Mouse monoclonal anti mmp 2, by Santa Cruz Biotechnology (1 mentions) Psd95, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti il 1β, by Santa Cruz Biotechnology (1 mentions) Rabbit anti mouse β actin, by Santa Cruz Biotechnology (1 mentions)

3 protocols using pen 2

Western Blot Analysis of Gamma-Secretase Complex

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Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris, pH8.0, 150 nM NaCl, 0.1% vol/vol Nonidet P-40, and 0.5% wt/vol deoxycholic acid) containing protease inhibitor mixture. Protein concentration was determined by the DC Protein Assay Kit (Bio-Rad). Antibodies used for Western blot are as follows: PS1-NTF and Nct (from our laboratory), PS1-CTF (MAB5232; Millipore), Aph1a (38-3600; Invitrogen), Pen2 (18189; Abcam), APP (MABN10; Millipore), and HA (18181; Abcam).

Wong E., Liao G.P., Chang J.C., Xu P., Li Y.M, & Greengard P. (2019). GSAP modulates γ-secretase specificity by inducing conformational change in PS1. Proceedings of the National Academy of Sciences of the United States of America, 116(13), 6385-6390.

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Characterization of APP processing in HEK293 cells

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HEK293 cells and HEK293 cells stably expressing human APP Swedish mutations (HEK293Swe) were cultured in DMEM (Hyclone) supplemented with 10% FBS (Gibco) and 1% penicillin/streptomycin (Gibco), in the absence and presence of 400 µg/mL G418 (Sigma), respectively.
Primary cortical neurons from embryonic day 17 (E17) rat pups were maintained in neurobasal medium supplemented with B27 and 0.8mM glutamine.
Antibodies used in these experiments include: NeuN (mouse mAb, Millipore), Nicastrin (mouse mAb, Abcam), Aph-1a (Invitrogen), Pen-2 (Abcam), Cleaved Notch1 (Val1744, Cell signaling), Anti-Notch1 intracellular domain antibody (rabbit pAb, Abcam), FLAG (mAb, clone M2, Sigma), GluR1 (mAb, Chemicon), GluR2 (pAb, Millipore), NR1 (mAb, BD Biosciences), α-tubulin (mAb, Sigma), β-actin (mAb, Sigma), Myc (9E10, Santa Cruz Biotechnology), Aβ (6E10, Covance); The rabbit polyclonal antibodies against PS1 loop (Thinakaran et al., 1996 (link)) and SNX27 (Balana et al., 2011 (link)) were described previously; The mouse monoclonal antibody (clone 3D5) against BACE1 was described previously (Zhao et al., 2007 (link)); The rabbit polyclonal antibody 369 against the APP C-terminus (Xu et al., 1997 (link)), Anti-Nicastrin (Ru716) and Anti-PS1 NTF antibody (Ab14) was developed in our laboratory.

Wang X., Huang T., Zhao Y., Zheng Q., Thompson R.C., Bu G., Zhang Y.W., Hong W, & Xu H. (2014). Sorting Nexin 27 Regulates Aβ Production through Modulating γ-secretase Activity. Cell reports, 9(3), 1023-1033.

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Western Blotting of Alzheimer's Markers

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Western blotting was carried out according to the manufacturer’s instructions and previous studies [30 (link), 31 (link)]. Cerebral cortex and hippocampus extracts were loaded onto 10-16% Tris/tricine SDS gels and transferred to nitrocellulose membranes prior to overnight incubation with one of the following primary antibodies: BACE, BACE1, sAPPβ, PS1, NCT, Aph-1α, Pen-2, p-Ser199, p-Ser202, p-Thr205, p-Thr231, p-Ser396, p-Ser404, HT7, p25, p35, and Cdk5 (purchased from Abcam, Cambridge, MA, USA). MAP1, SYP, and PSD95 were obtained from Cell Signaling Technology, Inc. USA. Mouse monoclonal anti-IL-1β, mouse monoclonal anti-MMP-2, mouse monoclonal anti-MMP-9, and goat anti-mouse IgG labeled with biotin were procured from Santa Cruz Biotechnology, Inc. USA. Rabbit anti-mouse β-actin was also obtained from Santa Cruz Biotechnology, Inc. USA. The optical densities of the specific bands were achieved by image analysis software (HPIAS 2000, Tongji Qianping Company, Wuhan, China).

Zhao Y., Wang C., He W, & Cai Z. (2022). Ameliorating Alzheimer’s-like Pathology by Minocycline via Inhibiting Cdk5/p25 Signaling. Current Neuropharmacology, 20(9), 1783-1792.

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