Annexin 7aad pe apoptosis detection kit

Twenty-four hours after CLP, tissue samples from lung and intestine were assessed with the transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) staining with a fluorescence detection kit (In Situ Cell Death Detection Kit; Fluorescein, Roche, Switzerland). The sections were observed with a fluorescence microscope, and representative fields were chosen for application. Apoptotic cells were detected by Annexin 7AAD/PE Apoptosis Detection Kit (BD Biosciences) following the manufacturer’s recommendations. The cells were analyzed with a flow cytometry (ACEA Biosciences,Inc.). The sum of early and late apoptotic cell percentage was defined as apoptotic cell percentage.

The apoptotic cells were detected by Annexin 7AAD/PE apoptosis detection kit (BD Biosciences Inc., USA) following the manufacturer's instructions. The collected cells were washed twice with ice-cold PBS and centrifuged at 1500 rpm for 5 min at 4°C. The cells were analyzed by flow cytometry (BD Biosciences Inc., USA). The apoptotic cell percentage was defined as the sum of the early and late apoptotic cell percentage.