Qrt pcr kit

Manufactured by GeneCopoeia

Sourced in United States

The QRT-PCR kit is a laboratory equipment product designed for the detection and quantification of specific DNA or RNA sequences through real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) technology. The kit provides the necessary reagents and protocols to perform this analytical method.

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Lab products found in correlation

7500 pcr system, by Thermo Fisher Scientific (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Rnapure total rna fast extraction kit, by BioTeke (1 mentions) β actin, by GeneCopoeia (1 mentions) Magnetic separation, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

All-in-One™ miRNA qRT-PCR Detection Kit (267 citations) MiRNA qRT-PCR Detection Kit (14 citations) All-in-One miRNA qRT-PCR Detection Kit 2.0 (6 citations) QRT-PCR Detection Kit (6 citations) MiRNA qRT-PCR kit (6 citations) MRNA qRT-PCR detection kit (3 citations) All-in-One miRNA quantitative reverse transcription- (qRT-) PCR detection kit (3 citations) All-in-OneTM miRNA qRT-PCR kit (3 citations) Lenti-Pac HIV qRT-PCR Titration Kit (2 citations) All‐in OneTM miRNA qRT‐PCR Detection Kit 2.0 (2 citations)

2 protocols using qrt pcr kit

Quantification of miR-100 Expression

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Total RNA was extracted from cultured cells, MVs, or lung tissues using the RNApure Total RNAFast Extraction Kit (BioTeke, Beijing, China) and reverse-transcribed to cDNA using a PrimeScript RT Reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol. The expression levels of miR-100 were determined using the miRNA quantitative real-time PCR (qRT-PCR) kit (GeneCopoeia, Rockville, MD, USA) in Applied Biosystems 7500 PCR system. The primers for miR-100 were synthesized by Sangon Biotechnology (Shanghai, China). Relative expression of miR-100 was calculated by the 2^−ΔΔCt method and normalized to U6.

Chen W.X., Zhou J., Zhou S.S., Zhang Y.D., Ji T.Y., Zhang X.L., Wang S.M., Du T, & Ding D.G. (2020). Microvesicles derived from human Wharton’s jelly mesenchymal stem cells enhance autophagy and ameliorate acute lung injury via delivery of miR-100. Stem Cell Research & Therapy, 11, 113.

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Vitamin D Receptor Expression Regulation

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1ɑ,25(OH)₂D₃ (Abcam, USA) was reconstituted in 100% ethanol and stored, protected from light, at -20°C. Anti-vitamin D receptor (VDR) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). IMag Cell Separation System was from MiltenyiBiotec, Germany. The miRNeasy kit for RNA isolation was from Qiaqen, USA. 1×SYBR Master Mix was from GeneCopoeia, USA. The qRT-PCR kit was also from GeneCopoeia, USA. Magnetic separation was from MiltenyiBiotec, Germany. The primers of miRNA-10a (ID: hsmq-0760), miRNA-125a (ID: hsmq-0483), miRNA-342 (ID: hsmq-0481), miRNA-374b-5p (ID: hsmq-0585), miRNA-377 (ID: hsmq-0255), miRNA-410 (ID:hsmq-0161), U6 (ID:hsnRNAU6), and VDR (ID:Hs-QRP-40488), CYP27B1 (ID:Hs-QRP-30404), CYP24A1 (ID:Hs-QRP-30402), and β-actin (ID:Hs-QRP-20056) were designed by GeneCopoeia USA.

Chen D.J., Li L.J., Yang X.K., Yu T., Leng R.X., Pan H.F, & Ye D.Q. (2017). Altered microRNAs expression in T cells of patients with SLE involved in the lack of vitamin D. Oncotarget, 8(37), 62099-62110.

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