Phase distribution of the cell cycle was determined by flow cytometry. Cell cycle analysis was performed as described by Ndebele et al. [44 ]. HT-29 cells were cultured in 13 × 100 mm plates at a density of 3 × 105/plate. The cells were treated with ATO at various concentrations for 24 h. Control cells (untreated) were cultured in medium alone. The cells were washed twice with cold PBS and harvested. The cell pellets were suspended in 250 μL of propidium iodide (PI) solution and incubated at 4°C in the dark for 1 h at a density of 1 × 106 cells/ml. The cell cycle distribution was measured using FACS Vantage flow cytometry system and Cell-Quest software (Becton-Dickinson, San Jose, CA).
Facs vantage flow cytometry system
Manufactured by BD
The FACS Vantage flow cytometry system is a high-performance instrument designed for advanced cell analysis and sorting. It provides precise measurement and separation of cells or particles based on their physical and fluorescent characteristics.
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2 protocols using facs vantage flow cytometry system
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Cell Cycle Analysis by Flow Cytometry
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Cell Cycle Analysis by Flow Cytometry
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Phase distribution of the cell cycle was determined by flow cytometry. Cell cycle analysis was performed as described by Ndebele et al. [44 ]. HT-29 cells were cultured in 13 × 100 mm plates at a density of 3 × 105/plate. The cells were treated with ATO at various concentrations for 24 h. Control cells (untreated) were cultured in medium alone. The cells were washed twice with cold PBS and harvested. The cell pellets were suspended in 250 μL of propidium iodide (PI) solution and incubated at 4°C in the dark for 1 h at a density of 1 × 106 cells/ml. The cell cycle distribution was measured using FACS Vantage flow cytometry system and Cell-Quest software (Becton-Dickinson, San Jose, CA).
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