We purchased 20(S)-ginsenoside Rg3 from the Ambo Institute (Daejon, Korea) and thrombin and all materials for platelet aggregation from Chrono-Log Corporation (Havertown, PA, USA). We purchased all materials for buffer solution and Rp-8-Br-cAMPS, Rp-8-Br-cGMP, p-chlorophenylthio (CPT)-cAMP, 8-Br-cGMP from Sigma (St. Louis, MO, USA) and Fura 2-acetoxymethyl (AM) from Invitrogen (Eugene, OR, USA). Thapsigargin and cAMP/cGMP enzyme immunoassay kit were obtained from Cayman Chemical (Ann Arbor, MI, USA). Anti-IP3-receptor type I, anti-phosphor-IP3-receptor type I (Ser1756), anti-extracellular signal-regulated kinase (ERK) (1/2), anti-phosphor-ERK (1/2), anti-rabbit IgG-horseradish peroxidase, and cell lysis buffer were obtained from Cell Signaling (Beverly, MA, USA). Anti-β-actin was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse monoclonal to CD62P (p-selectin) antibody was purchased from Biolegend (San Diego, CA, USA). Polyvinylidene difluoride (PVDF) membrane and enhanced chemiluminesence solution (ECL) were purchased from General Electric Healthcare (Chalfont St. Giles, Buckinghamshire, UK).
Rp 8 br camps
Manufactured by Merck Group
Sourced in United States, Japan
Rp-8-Br-cAMPS is a synthetic compound that functions as a cyclic adenosine monophosphate (cAMP) analog. It is commonly used as a research tool in biochemical and cell-based studies to investigate the role of cAMP signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
8 br cgmp, by Merck Group (1 mentions)
Cell lysis buffer, by Cell Signaling Technology (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti rabbit igg horseradish peroxidase, by Cell Signaling Technology (1 mentions)
Thapsigargin, by Cayman Chemical (1 mentions)
Fura 2 acetoxymethyl am, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal to cd62p p selectin antibody, by BioLegend (1 mentions)
Thrombin, by Chrono-Log (1 mentions)
Phytohemagglutinin (pha), by Thermo Fisher Scientific (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
Glomax 96 well luminometer, by Promega (1 mentions)
L lactate, by Merck Group (1 mentions)
Forskolin, by Merck Group (1 mentions)
Pacap 38 amino acid form, by Peptide Institute (1 mentions)
Ar c155858, by Merck Group (1 mentions)
Gf109203x, by Fujifilm (1 mentions)
3 3 diaminobenzidine (dab), by Fujifilm (1 mentions)
Phorbol 12 myristate 13 acetate, by Fujifilm (1 mentions)
3 protocols using rp 8 br camps
1
Ginsenoside Rg3 Regulation of Platelet Function
2
Modulating IL-2 Expression in Jurkat Cells
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Jurkat E6 cells expressing an IL-2::Luciferase reporter construct (44 (link)) were either pre-treated for 30 min with the PKA inhibitors Rp-8-Br-cAMPS (200 nM) or H89 (1μM; both Sigma Aldrich) or transduced with an empty control-vector or the human PDE4A cDNA. 2 × 106 cells were activated with PMA (1 × 10−7 M, Sigma Aldrich) and PHA (12.5 μg/mL; Thermo Scientific, Waltham, MA). After 6 h, cells were lysed and luciferase activity was on a GloMax 96 well luminometer (Promega, Madison, WI).
3
PACAP and VIP Signaling Mechanisms
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PACAP (38 amino acid form) and VIP were purchased from Peptide Institute Inc.
(Osaka, Japan). Max was kindly donated by Dr. M Tajima (Shiseido, Japan). DAB, GF109203X and phorbol 12-myristate 13-acetate (PMA) were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Forskolin and AR-C155858 were from Merck Millipore (Darmstadt, Germany). H89 was from Seikagaku Co. (Tokyo, Japan).
L-lactate and Rp-8-Br-cAMPS were from Sigma-Aldrich Co. LLC (St. Louis, MO). this study were determined according to our previous reports 17, 27, 28 .
(Osaka, Japan). Max was kindly donated by Dr. M Tajima (Shiseido, Japan). DAB, GF109203X and phorbol 12-myristate 13-acetate (PMA) were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Forskolin and AR-C155858 were from Merck Millipore (Darmstadt, Germany). H89 was from Seikagaku Co. (Tokyo, Japan).
L-lactate and Rp-8-Br-cAMPS were from Sigma-Aldrich Co. LLC (St. Louis, MO). this study were determined according to our previous reports 17, 27, 28 .
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