Cysteine, sodium di-hydrogen phosphate, sodium sulphide, resazurin, salivary α-amylase, and pepsin from porcine bile acids (porcine bile extract) were provided by Sigma-Aldrich (Darmstadt, Germany). Pancreatin from porcine pancreas was provided by Alpha Aesar (Lancaster, UK).
Salivary α amylase
Manufactured by Merck Group
Sourced in United States, Germany
Salivary α-amylase is an enzyme found in human saliva that catalyzes the hydrolysis of starch into maltose and dextrin. It is a key component in the initial stages of the digestion of dietary carbohydrates.
Automatically generated - may contain errors
Lab products found in correlation
Porcine pepsin, by Merck Group (4 mentions)
Pancreatin, by Merck Group (3 mentions)
Porcine pancreatic lipase, by Merck Group (3 mentions)
Porcine bile extract, by Merck Group (3 mentions)
Porcine pancreatic α amylase, by Merck Group (3 mentions)
Sodium sulphide, by Merck Group (2 mentions)
Resazurin, by Merck Group (2 mentions)
Cysteine, by Merck Group (2 mentions)
Chymotrypsin, by Merck Group (2 mentions)
Trypsin, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Sodium dihydrogen phosphate, by Merck Group (1 mentions)
Pancreatin from porcine pancreas, by Thermo Fisher Scientific (1 mentions)
Bile salt, by HiMedia (1 mentions)
Hemin, by HiMedia (1 mentions)
Vitamin k1, by HiMedia (1 mentions)
Acetic acid, by RCI Labscan (1 mentions)
Cytidine, by Merck Group (1 mentions)
Aldolase, by Merck Group (1 mentions)
7 protocols using salivary α amylase
1
Enzyme-Mediated Biomolecule Extraction
2
Simulated Biofluids Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals used for the analysis of simulated salivary fluids, simulated gastric fluids and simulated intestinal fluids were of analytical grade and were made available from RCI Labscan (Bangkok, Thailand). Salivary α-amylase and pancreatin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bile salts, hemin, vitamin K1, cysteine HCl and the appropriate media were purchased from HiMedia (Nashik, India). Standard short chain fatty acids (SCFA), including propionic acid, butyric acid, acetic acid and lactic acid, were of HPLC grade and were obtained from RCI Labscan.
3
Analysis of Compounds in Gastrointestinal Digestion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chlorpyrifos (CPF), diazinon (DZN), and trifluoroacetic acid were purchased from Sigma-Aldrich, Co (St. Louis, MO, USA). Methanol, ethanol, and phosphoric acid were purchased from Scharlab, S.L (Barcelona, Spain). Sodium hydroxide, Tris, urea, thiourea, calcium chloride anhydrous, and chloride acid were from Panreac Qumica S.A (Barcelona, Spain) and iron sulphate was from BDH laboratory (Kuwait, Kuwait). Myosin, aldolase, bovine serum albumin, ovoalbumin, myoglobin, cytidine, salivary α-amylase, porcine pepsin, porcine pancreatic α-amylase, porcine pancreatic lipase, and porcine bile extract used in the simulation of GI digestion were purchased from Sigma-Aldrich, Co. (St. Louis, MO, USA).
trypsin and chymotrypsin enzymes were from Fluka (Sigma-Aldrich, Co., St. Louis, MO, USA). The trypsin used in mass spectrometry assays was obtained from Promega, Co (Madrid, Spain).
trypsin and chymotrypsin enzymes were from Fluka (Sigma-Aldrich, Co., St. Louis, MO, USA). The trypsin used in mass spectrometry assays was obtained from Promega, Co (Madrid, Spain).
4
In Vitro Digestion and Antioxidant Evaluation of Chicken Breast
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chicken breast was obtained from a local market 24 h after slathering (Valencia, Spain) . Enzymes for in vitro digestion: salivary α-amylase, porcine pepsin, porcine pancreatic α-amylase, porcine pancreatic lipase, and porcine bile extract were obtained from Sigma Aldrich, Co. Ltd (St. Louis, MO, USA), whereas trypsin and chymotrypsin were from Fluka (Sigma-Aldrich, Co., St. Louis, MO, USA).
2,2′-Diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide, ferric chloride, trichloroacetic acid (TCA), 2,6-di-tertbutyl-4-methylphenol (BHT, B1378), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), fluorescein,
2,2′-Diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide, ferric chloride, trichloroacetic acid (TCA), 2,6-di-tertbutyl-4-methylphenol (BHT, B1378), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), fluorescein,
5
Biochemical Analysis of Antioxidant Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Trolox ((±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), gallic acid, acetic acid, propionic acid, butyric acid, Folin-Ciocalteu reagent, sodium hydroxide, hydrochloric acid, ferric chloride hexahydrate, sodium acetate, potassium chloride, potassium dihydrogen phosphate, sodium monohydrogen carbonate, sodium chloride, magnesium chloride hexahydrate, ammonium carbonate, calcium chloride dihydrate, sodium dihydrogen phosphate, tryptone, cysteine, sodium sulphide, resazurin, salivary α-amylase, porcine pepsin, bile acids ( porcine bile extract), pancreatin and ethanol were from Sigma-Aldrich (Darmstadt, Germany).
6
Probiotic Encapsulation and Gut Simulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The L. rhamnosus LGG strain was kindly provided by Chr. Hansen, Bayswater, VIC, Australia. Na-alginate, Fructooligosaccharides-FOS, hi-maize resistant starch, skim milk powder and enzymes (salivary α-amylase, porcine pepsin, pancreatin), acetone, HCl, acetic acid were bought from Sigma Aldrich (NSW, Australia). Whey protein concentrate was gifted by Murray Goulburn Co-operative Co. Limited (Victoria, Australia). The nutrient agar & broth, selective media DeMan, Rogosa and Sharpe (MRS), AnaeroGen sachets, beef extract, yeast extract, protease peptone, bile salts, and trichloroacetic acid were purchased from Thermo Fisher (Thermo Fisher Scientific Pty Ltd, VIC. Australia). NaOH, phosphate-buffered saline, CaCl 2 , dextrose, K 2 HPO 4 , (NH 4 ) 2 SO 4 , MgSO 4 •7H 2 O, NaCl, KCl, NaHCO 3 , MgCl 2 (H 2 O) 6 , (NH 4 ) 2 CO 3 , potassium acetate, aluminium chloride, potassium persulfate were procured from the Chem-Supply Pty Ltd, (Melbourne, Australia)
7
In vitro gastrointestinal digestion protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Enzymes used for the in vitro gastrointestinal digestion: salivary α-amylase, porcine pepsine, porcine pancreatic α-amylase, porcine pancreatic lipase, and porcine bile extract were purchased from Sigma-Aldrich, Co. (St. Louis, MO, USA). Trypsin and chymoTrypsin enzymes were from Fluka (Sigma-Aldrich, Co., St. Louis, MO, USA).
Regarding chemicals used in the antioxidant assays, 2,2-diphenyl-1-picrylhydrazyl
Regarding chemicals used in the antioxidant assays, 2,2-diphenyl-1-picrylhydrazyl
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!