HepG2, HepG2-miR-122 (36 (link)) (obtained from Dr Matthew Evans, Icahn School of Medicine at Mount Sinai), Huh-7.5 and Huh-7.5-ΔmiR-122 (37 (link)) cells (obtained from Dr Charles Rice, Rockefeller University) were maintained in DMEM medium with 10% fetal bovine serum. Sofosbuvir (2′-deoxy-2′-α-fluoro-β-C-methyluridine-5′-monophosphate) was purchased from ChemScene, LLC (Monmouth Junction, NJ). Final dilutions contained 0.1% DMSO. Cell viability was assessed with WST-1 reagent (MilliporeSigma, Burlington, MA) using the manufacturer’s suggested protocol.
Sofosbuvir 2 deoxy 2 α fluoro β c methyluridine 5 monophosphate
Manufactured by ChemScene
Sofosbuvir (2′-deoxy-2′-α-fluoro-β-C-methyluridine-5′-monophosphate) is a nucleotide analog that inhibits the hepatitis C virus (HCV) NS5B polymerase.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sofosbuvir 2 deoxy 2 α fluoro β c methyluridine 5 monophosphate
1
Evaluating Antiviral Activity in Liver Cell Lines
2
Sofosbuvir Antiviral Assay in Huh-7.5 and MEFs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Huh-7.5, FT3-7 (a clonal derivative of Huh7 cells), wild-type and Ago2 knockout murine embryonic fibroblasts (MEFs) were maintained as described previously (8 (link),19 (link)). Sofosbuvir (2΄-deoxy-2΄-α-fluoro-β-C-methyluridine-5΄-monophosphate) was obtained from CHEMSCENE, LLC. Final dilutions contained 0.1% dimethyl sulfoxide.
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