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Anti pdcd4

Manufactured by Merck Group
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Anti-PDCD4 is a laboratory reagent that can be used for the detection and quantification of the PDCD4 (Programmed Cell Death 4) protein. PDCD4 is a tumor suppressor protein involved in various cellular processes. The Anti-PDCD4 product can be used in research applications, such as western blotting, immunohistochemistry, and ELISA, to study the expression and localization of PDCD4 in different cell types and experimental models.

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Lab products found in correlation

Goat anti mouse igg hrp, by Santa Cruz Biotechnology (2 mentions) Anti sma clone 1a4, by Merck Group (2 mentions) Anti actinin h 2, by Santa Cruz Biotechnology (2 mentions) Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (2 mentions) Ripa buffer, by Merck Group (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Chemidoc xrs system, by Bio-Rad (2 mentions)

2 protocols using anti pdcd4

1

Immunoblotting of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell extracts were prepared with radioimmunoprecipitation assay (RIPA) buffer (Sigma) supplemented with a protease-inhibitor cocktail (Roche). For immunoblot analysis, protein samples were resolved on a 10% polyacrylamide Bis-Tris gel and transferred onto a nitrocellulose membrane. The following primary antibodies were used: anti-SMA (clone 1A4, Sigma), anti-PDCD4 (SAB1407349 Sigma), anti-SUMO (GeneScript A00640) and anti-actinin (H-2) (sc-17829, 1:2000, Santa Cruz Biotechnology). Goat anti-rabbit IgG-HRP (1:3000, Santa Cruz Biotechnology) and goat anti-mouse IgG-HRP (sc-2005, 1:3000, Santa Cruz Biotechnology) were used as secondary antibodies. All Western blots were analyzed with the ChemiDoc XRS+ System (Bio-Rad).
Chen Y., Yang F., Zubovic L., Pavelitz T., Yang W., Godin K., Walker M., Zheng S., Macchi P, & Varani G. (2016). Targeted inhibition of oncogenic miR-21 maturation with designed RNA-binding proteins. Nature chemical biology, 12(9), 717-723.
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2

Immunoblotting of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell extracts were prepared with radioimmunoprecipitation assay (RIPA) buffer (Sigma) supplemented with a protease-inhibitor cocktail (Roche). For immunoblot analysis, protein samples were resolved on a 10% polyacrylamide Bis-Tris gel and transferred onto a nitrocellulose membrane. The following primary antibodies were used: anti-SMA (clone 1A4, Sigma), anti-PDCD4 (SAB1407349 Sigma), anti-SUMO (GeneScript A00640) and anti-actinin (H-2) (sc-17829, 1:2000, Santa Cruz Biotechnology). Goat anti-rabbit IgG-HRP (1:3000, Santa Cruz Biotechnology) and goat anti-mouse IgG-HRP (sc-2005, 1:3000, Santa Cruz Biotechnology) were used as secondary antibodies. All Western blots were analyzed with the ChemiDoc XRS+ System (Bio-Rad).
Chen Y., Yang F., Zubovic L., Pavelitz T., Yang W., Godin K., Walker M., Zheng S., Macchi P, & Varani G. (2016). Targeted inhibition of oncogenic miR-21 maturation with designed RNA-binding proteins. Nature chemical biology, 12(9), 717-723.
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