Annexin 5 fitc apoptosis kit

MDA-MB-231 (derived from Caucasian American TNBC) and MDA-MB-468 (derived from African American TNBC) were purchased from American Type Culture Collection (ATCC). Dulbecco’s modified Eagle’s medium (DMEM) high glucose; fetal bovine serum heat inactivated (FBS-HI), penicillin/streptomycin and Hank’s Balanced salt solution (HBSS) were obtained from Genesee Scientific (San Diego, CA, USA). Dimethyl sulfoxide (DMSO), butein, and Alamar Blue^® were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Human cytokine antibody arrays (Cat# AAH-CYT-6-4), ELISA assays for MCP-1 (Cat# ELH-MCP1–1), Annexin V-FITC apoptosis Kit (Cat# 68FT-AnnV-S100), and tumor necrosis factor alpha (TNF-α) were purchased from RayBiotech (Norcross, Ga, USA). PCR primers, iScript advanced reverse transcriptase kit, and Bradford reagent were purchased from Bio-Rad (Hercules, CA, USA). DNA-free^™ Kit (Cat # AM1907) from Life Technologies Inc. (Grand Island, NY, USA).). All reagents and plates for Western assays were purchased from ProteinSimple (San Jose, CA, USA). Primary antibodies were purchased from Cell Signaling (Danvers, MA, USA). The list of primary antibodies and their characteristics are described as follows (Table 1):